查询词典 cell complex

Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection. A signal significantly greater than background binding was observed from the third to the fifth round of selection on CHOEGFRGFP1 and CHOK1 cell, but a part of polyclonal scFv bound EGFR specially. About 45% of the selected clones contained a fullsized insert of 1 kb. One unique human antiEGFR scFv (F4scFv) was isolated by analyzing with cell ELISA and DNA sequencing.

结果 经过5轮筛选，细胞裂解液中洗脱出噬菌体效价有500倍以上增长；细胞ELISA结果显示多克隆pscFv与CHOEGFRGFP1细胞和CHOK1细胞均有明显结合，但有部分特异性结合EGFR；菌落PCR显示约45％克隆中含有完整的1kb scFv片断；经细胞ELISA、 DNA测序检测共获得1株EGFR特异性单链抗体，命名为F4scFv。

Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

It was expressed on trophoblastic cell, interstitial cell and endothelium cell of interstitial blood vessels of villi.

结果 ①所有的标本绒毛滋养层细胞、绒毛间质细胞、毛细血管内皮细胞都有HAV的存在。

Measure normal early pregnancy choria tissue and JAR cell by RT-PCR, LH/hCG receptor mRNA expression on both pregnancy choria tissue and JAR cell which is consistent with that be measured in ovarian granulosa cells, indicate that JAR cell and normal choria trophocyte presence LH/hCG receptor, hCG is one of the autocrine hormone.

二、用RT-PCR法可测到正常妊娠早孕绒毛组织、JAR细胞的LH／hCG受体mRNA表达，与正常卵巢颗粒细胞所表达的LH／hCG受体mRNA一致，提示JAR细胞与正常绒毛滋养细胞存在LH／hCG受体，hCG为其自分泌激素之一。

Trypan blue exclusion was used to measure the effect of TCS on cell growth and flow cytometry was used to detect the effects of TCS on cell apoptosis and cell cycle.

用台盼篮染色法检测TCS对细胞生长的影响，应用流式细胞术检测TCS诱导细胞凋亡的情况及对细胞生长周期的影响。

The second, many coccus in kidney, heart and pancreas were found besides liver cell degeneration, congestion of liver, epithelium cell degeneration, necrosis of tubuli tenales, kidney hemorrhage, cardiac muscle cell degeneration, heart hemorrhage.

病例2，肝细胞变性，肺淤血，肾小管上皮细胞变性、坏死，间质出血，心肌纤维变性，间质出血，肾、心、胰脏均可见到散在分布的球菌。

To get more information of the function of human Era on cell cycle, the complete control inducible mammalian expression system was used to express human Era mutant and analyzed cell cycle of uninduced (add 1% ethanol) and induced (10μM Pon A) cell lines by flowcytometer, the primary results demonstrated human Era mutant could inhibit apoptosis induced by ethanol, and Era S36N may stimulate the expression of Bcl-2 as detected by Western Blot.

在构建稳定高表达蜕皮激素受体基因的细胞株3V3的基础上，转染突变型人Era的表达载体进行压力筛选，对所得到的单克隆细胞株进行诱导与未诱导条件下细胞周期的分析，结果表明未诱导加酒精的对照细胞可以引起细胞凋亡，而Era诱导表达后可以显著抑制凋亡细胞的数量，Western表明Era S36N可以诱导细胞Bcl-2表达，提示人Era可能与细胞凋亡相关。

By means of the results of ordinal regression analysis, this study discovers phenomena as follows: in a single automobile which runs off the road, the main factors are such as the use of cell phone and seat belt, drinking alcohol, violative behavior, trip purpose, timing, visibility, and road quality; in a single automobile which hits islands, the key factors are consist of driver's license and qualification, the use of cell phone and seat belt, trip purpose, visible distance, road visibility, road quality, and limited speed; in a single automobile which hits the roadside objects, the primary factors include the use of cell phone and seat belt, driver's license and qualification, drinking alcohol, violative behavior, age, timing, trip purpose, and region.

由顺序回归分析结果可知影响事故严重度的关键因素。在冲出路外的单一小客车事故中，关键因素为行动电话使用情形、安全带使用情形、饮酒情形、违规行为、旅次目的、时段、道路能见度和铺面品质；在撞路中物体的单一小客车事故中，关键因素为驾驶执照、驾驶资格、行动电话使用情形、安全带使用情形、旅次目的、道路视距、道路能见度、铺面品质和速限；在撞路旁物体的单一小客车事故中，关键因素为行动电话使用情形、安全带使用情形、驾驶执照、驾驶资格、饮酒情形、违规行为、年龄、时段、旅次目的和地区。

The reacting tite with NDPK-A was 1:800 and theantisera was specific.Conclusions: The purified rhNDPK-A protein exists mainly in the form of hexamer;rhNDPK-A protein can promote the survival of spleen cell and may take part in theadjustment of cell growth by the way of changing cell cycle.

ELISA法检测所制备的抗体的反应效价为：1：800，western blotting试验结果证实，该抗血清具备较好的特异性，能与rhNDPK-A蛋白发生反应，结论：rhNDPK-A蛋白可能主要以六聚体形式存在，直接观察的蛋白结构与文献报道的X衍射结果相似；rhNDPK-A蛋白对正常小鼠的脾细胞的存活有促进作用，推测很可能以信号转导的方式调控细胞周期从而达到对细胞生长的调节作用。

Solar cell of silicon -membrane type is therefore regarded as the development direction for the future. Solar cell of noncrystal silicon -membrane type will play it s important role, with continual betterment. And solar cell of nanosilicon membrane type will also take an main part because of it s advantage.

非晶硅薄膜太阳能电池虽在性能上具有不少的缺点，但是随着薄膜沉淀技术的提高以及工艺上的不断改进会使它在太阳能领域在有一席之地，后起之秀纳米硅薄膜太阳能电池依靠其本身的优越性以及纳米技术的进展将会成为一个重要的竞争者。

The studies on the developing of new media are very important to the increasing of the area density of HDD and to the implementation of hybrid recoding technology.

光磁混合记录方法，是一种可以突破超顺磁极限的限制，并进一步提高硬盘记录密度和读写速率的一种新型超高密度信息存储方式。

In io, mTOR inhibition promotes T cell anergy under conditions that would normally induce priming.

体内研究中，在正常可激发免疫反应的条件下，抑制mTOR可促使T细胞无能。