查询词典 cartilage cell

Objective To explore the influences of different inductive methods on cartilage repair by tissue engineered cartilage with the seed cell of mesenchymal stem cells.

目的：以兔骨髓间质干细胞为种子细胞，探讨不同诱导方法对组织工程移植物修复关节软骨缺损的效果及退化的影响。

OBJECTIVE: To prepare human articular cartilage acellular matrix so as to provide a methodological basis for further study of articular cartilage acellular matrix as cell scaffold materials.

目的：制备人关节软骨脱细胞基质，为进一步研究关节软骨基质作为细胞外支架材料提供方法学资料。

In recent years,masenchymal stem cells have been applied in cartilage tissue engineering and they have shown significant potenital for carriage repair in animal models,and are being used as an ideal cell source in cartilage tissue engineering.

：间充质干细胞具有高度自我新能力，能够分化各类型的细胞近20年间充质细胞在软骨组织工程中得到应用，动物实验显示了良好的软骨修复潜能，成为软骨组织工程的理想的细胞来源。

TCP is an ideal cell scaffold for cartilage tissue engineering. Tissue-engineered cartilage based on MSCs and β-TCP is promising in clinical application and commercialization.

TCP是软骨组织工程较为理想的支架材料，以其为支架，以自体MSCs为种子细胞构建的组织工程化生物性软骨具有极大的临床应用潜力和广阔的产业化开发前景。

Now MSC become the chief seed-cell in cartilage tissue engineer, but the technology to stimulate MSC differentiate into chondroblast is not very successful,,the standard technology is culture the cell-ball in centrifuge tube,the reconstructured cartilage-like tissue is not achieve the mechanics of hyaline cartilage.

软骨组织工程的材料学研究主要体现在两类基质材料方面的进展，一类为天然基质材料，如胶原、壳聚糖、纤维素等；一类为人工合成可降解材料，如PGA、PLA等，但两者在来源、塑形能力及细胞识别信号等方面存在不足。

TyPe II collagen induced arthritisln the rat ank1e joint andoVathumin as antigen induced arthritis WA in the rabbit knee joint wereestab1ish2 Qualitative evaluation of me in skin, muscle, synovium, cedilagearound joint and blood was performed by OMA3 The CIA rats were treated on day 7 after hind paw swelling and erythemaAnimals were injected intravenously with ase at a dose of 10mg/kg,tWenty minuots 1ater, one ankle of the rats random1y assigned was exPosedlaser irradiation at l00J/cm fOr l000 seconds, and another ankle wasM grouP wihout laser The other two groups is unmanipulatedcontrol group and untreated CIA group Bimaleolar ankle widthmeasuremellts were taken in all animals every tWo days using amicrometer The histopathology of the ank1e Joint was assessed at day 21after disease onset4 The pro1iferating cell nuclear antigen WCNA of CIA treated by PDT andthe HMME group without laser was doterdrined by immunohistochemiStry5 The AfA rabbits were treated on day 7 after knee swelling and erythemaThe theraPy invo1ved lntravenous injection of l0mg/kg HMME, fOl1owedby 20 minues period in dim light, and transdermal light treatment with\l00 J/cm2 fOr l000 seconds The inner sides of the treated Anees wereirradiated at first, and then the outer side did 24 hours later, the synovialtissue of the Anees joint were removed and in situ cel1 aPoptosis wasdetCCted With tednal deoxync1eotidyl transferase-mediated dUTP nickend labelingR6suIt8:l The pathologic changes of CIA and AIA include subsynovial inflammation,opovial hyPerplasia, pannus formation, cartilage and bone destructionresemble RA.2 The studies demonstrated that there are different uptake of HMME withinskin, muscle, synovium, cartilage and b1ood, and the synovium cou1draPidly uPtake more ase than skin and cartilage at the firSt 30 minuesaller intravenous injection of HMME3 The bimaleolar anke width had no different among PDT treated group,H group withollt 1aser and untreated CIA group But hlstologicalevaluation showed statiStical1y significallt reductions in synovialhyperplasia, pannus formation and cart1lage reosion, bone destruction andtotal score in PDT treated group4 Image analysis showed that the ratlo bforeen the areas of the coufltedobect to that of the entire area in PDTtreated grOup is lower than that in conirol group, but the integrated oPticaldensity had no different between the two groups5 Imape analysis showed that the ratio between the area of the countedobject to that of the e

治疗组在大鼠出现踝关节红肿后1周，炎症达到高峰时进行PDT治疗。随机治疗大鼠一侧的踝关节，另。2。一一侧作单纯HMME 对照。治疗方法是大鼠麻醉后尾静脉注入 HMME10ngkg，20分钟后踝关节照光，激光波长627.sum，功率密度 100mwcm'，照射时间1000秒，能量密度100）／。治疗后避光喂养72 小时。隔日一次测量大鼠的踝关节左右横径，治疗后两周取关节进行病理d 观察。 4。大鼠CIA模型用上述方法进行PDT治疗后，治疗组和单纯HMME 组用兔疫组化SP法检测石蜡切片的核增殖抗原。 5。兔AIA模型在关节炎出现第七天进行PDT治疗，随机治疗一侧膝关节，另一侧作自身对照。兔耳静脉注入I'arrainrelomg／Kg，20分钟后，膝关节用金蒸气激光照射，激光能量密度100）儿旷。24 ／J'时后取膝关节滑膜作病理检查，并用脱氧核昔酸末端转移酶介导的缺口末端标记法原位检测凋亡细胞。结果： 1。模型观察：CIA大鼠炎症高峰期滑膜下炎细胞浸润明显，滑膜细胞明显增殖，炎症达到高峰后二周，血管缀形成，并侵蚀和破坏软骨和骨， CIA模型病理改变与人类RA相似。兔AIA模型膝关节滑膜病理可见滑膜细胞增生，滑膜下炎细胞浸润，也与人类RA滑膜改变相似。 2。关节周围组织中光敏剂含量的测定结果表明，各组织对HMME 的吸收速度和吸收量不同，荧光值一时间曲线不同，滑膜组织比皮肤和软骨对 HMME的吸收多，在 2 0分钟时即有明显差异。 3.PDT对CIA模型的治疗结果表明：PDT治疗后关节炎组、单纯 HMME组和治疗组踝关节左右横径统计学检验差异没有显著性，但病理评分PDT治疗组滑膜增生、血管资形成及软骨破坏、骨破坏和总分比关节炎对照组和HMME对照组好，统计学检验差异有显著性。。3＿军医进修学院硕士学位论文中文摘要 4.PDT治疗组PCNA阳性细胞较对照组少，图像分析结果表明面密度（阳性染色的面积总和与统计视野面积的比值）治疗组小于对照组，统计学检验差异有显著性。。 5.PDT治疗组凋亡阳性细胞较对照组明显增多，图像分析结果单位视野内阳性细胞数和面密度PDT治疗组高于对照组，统计学检验差异有显著性。凋亡细胞核直径PDT治疗组较小，与对照组相比，统计学检验差异有显著性。结论：二。CIA、AIA的病理改变类似人类RA，可作为研究RA病因、发病机制、检查及治疗方法的模型。 2。各组织对HMME的吸收速度和吸收量不同，滑膜组织比皮。

Both Fas and NO can lead chondrocyte apoptosis respectively and cause articular cartilage destruction. IGF-Ⅰ, TGF-β, bFGF, BMP and other growth factors are polypeptide agents that can influence cell activity by signal convection. They can accelerate chondrocyte proliferation and proteoglycan synthesis, play the local regulation action on formation and plerosis of bone and cartilage tissue by autocrine or paracrine. They have the ability to induce cartilage formation. Some investigations showed that growth factors can influence chondrocyte metabolism, synthesis of specific Ⅱ type collagen and proteoglycan by co-operation and inhibition. 1. 3 Situation of OA therapeutics The therapeutic methods of OA mainly comprised non-drug treatment, drug treatment, operation treatment, tissue and genetic engineering, et al. Drug treatment is the chief method among them.

若其活性发生改变，则将导致关节软骨基质成分的丢失和进行性破坏；软骨细胞凋亡与OA的发病密切相关，Fas与NO可各自独立介导软骨细胞凋亡，造成关节软骨破坏；IGF-Ⅰ、TGF-β、bFGF、BMP等生长因子是一组通过细胞间信号传递影响细胞活动的多肽因子，具有促进细胞生长、增殖与合成等作用，可通过自分泌或旁分泌方式对骨和软骨的形成和修复起局部调节作用，可促进软骨细胞增殖、分化与蛋白多糖的合成，具有较强的诱导软骨形成的能力，研究表明多种生长因子相互促进、相互抑制，以协同或拮抗方式影响软骨细胞代谢，影响软骨细胞特异性Ⅱ型胶原和蛋白多糖的合成分泌。

The etiology and pathogenesis is not very clear,may be due to excessive mechanical load or unbalanced distribution of cartilage wear,and then chondrocyte metabolic changes.Cell release a variety of catabolic enzymes that lead to aggrecan degradation,collagen network useless and cartilage unhomogeneity.

整合素作为细胞表面受体使整合素在细胞跨膜信号传递中起一定的作用，并且参与细胞的多种生理功能，如细胞的生长、发育、分化、凋亡等，同时参与多种病理过程，如淋巴细胞向炎症部位的聚集及肿瘤细胞的浸润等[2]。

The method of centrifuge tube culture can be used as a constructing technique of tissue engineered cartilage without scaffold material.Cartilage,articular; Histology; Biomedical engineering; Cell culture

结论在无支架材料条件下，采用离心管培养技术培养的人胚胎关节表面软骨细胞可以构建组织工程化关节软骨，而且接近正常关节软骨的形态和功能。

The laboratory research and chinical trials to prove.courage lcecontains active material that it can quickly penetrate through body's cell.so to directly provides for the cell,fill the skin intercellular space the crevice which is senile withered because of the cell stays benind supply the active elements necessary for the body cells metabolism,to activate the function of endothelium cells of blood vessels,promote the blood vessels balance, compose and secrete various blood vessels active elements,contains abundantsulfuric cartilage element,sulfuric amido etc.,all of fatty acid in human body.and it can accelerete the re-generation of blood cells,add the quantity of the hemoglobin and red blood corpuscle so to improve the blood parameter,and fundamentally improve the body cells metabolism and nutrition supply for all the organs,as a result,you will thoroughly refresh yourself and bloom vitality of youth.

实验室研究和临床试验证明，LCE所含活性物质能够迅速穿透人体细胞，直接为细胞供养，填补肌肤细胞间因细胞衰老干瘪而留下的空隙全面补充人体细胞新陈代谢所必须的活性物质，激活血管内皮细胞功能，促进血管平衡，合成和分泌多种血管活性物质：富含的硫酸软骨素、硫酸氨基葡萄糖等能改善和调节人体脂代谢，有效清除体内血液中多余的脂质和脂蛋白清除在血管壁内膜沉积的脂类物质，保持人体脂肪酸均衡：能够加快血液细胞更新，提高血液中的血红蛋白和红血球数量，改善血液参量，从根本上改善人体细胞的新陈代谢，改善人体各组织器官的营养供给，使人由内而外脱胎换骨，重新焕发青春活力。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。