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Level of CaM of leaves in Ginkgo and Ailanthus is sensitive to drought stress:theCaM level of Ginkgo first decreased and then increased,CaM level of Ailanthus went ondecreasing along with stress strengthen.

银杏和臭椿叶片CaM水平对干旱胁迫敏感:随着胁迫加强,银杏CaM水平出现先下降后上升的趋势,臭椿CaM水平持续下降。

By using GFP-CaM fusion protein, we have observed the detailed dynamic redistribution of CaM in HeLa cells during cytokinesis.

我们采用绿色荧光蛋白标记技术,重点观察了胞质分裂期HeLa细胞中GFP-CaM的动态分布;应用三极细胞模型研究了CaM的分布与分裂沟形成之间的关系;还通过在胞质分裂不同阶段抑制CaM的活性研究了CaM在整个胞质分裂过程中的作用机制。

The results indicated that the content of cytosolic CaM in cells treated with exogenous Ca2+ has increased indistinctively, while fluorescence intensity in cells treated with TFP decreased. So we believed that exogenous Ca2+ has little effect on the expression of CaM. High concentration of TFP can enter yeast cells and combine to CaM to make it inactive, which is the reason that TFP restrain the growth of yeast cells. 5mmol/L EGTA could completely arrest the cell proliferation of MFP7 after 28h, when the fluorescence intensity in cells wasobviously increased with flow cytometry and LSCM.

被较高浓度TFP处理过的MFP7胞内荧光强度则相对较弱,推测是因为TFP进入胞内与CaM结合从而使其失活,这也是高浓度TFP抑制酵母细胞增殖的原因。5mmol/L EGTA处理28h左右后,酵母细胞的生长被抑制在G1晚期,此时通过细胞流式法和在激光扫描共聚焦显微镜下观察到胞内荧光强度显著高于对照,说明CaM的表达水平在G1后期开始上升;回加10mmol/L Ca~(2+)处理24h后,细胞开始恢复生长,细胞流式法测定胞内荧光强度有所下降,表明多数细胞完成G1/S转换,进入生长对数期。

System speaking of the Master CAM, first must establish the components the geometry model, according to the components actual size, draws its processing part the contour line, does not need to draw the entire components the model, guaranteed the computation production the cutting tool way coordinates accuracy, and may use the Master CAM Chinese Library level the function, when determination cutting tool way, transfers or the hideaway, the selective treatment needs contour line; Next, the formulation processing craft and the definite craft parameter, chooses the cutting tool, the processing semi finished materials, the work piece origin of coordinates again and so on, establishes the work piece coordinates system, definite work piece coordinate system and engine bed coordinate system relative size, thus obtains the components processing correct cutting tool way; Finally, the simulation function which system provides using the Master CAM, the realization simulation processing.

对于Master CAM系统而言,首先要建立零件的几何模型,根据零件的实际尺寸,画出其加工部分的轮廓线,无需画出整个零件的模型,以保证计算生成的刀具路径坐标的正确性,并可利用Master CAM中图层的功能,在确定刀具路径时,加以调用或隐藏,来选择加工需要的轮廓线;其次,制定加工工艺并确定工艺参数,再选择刀具、加工毛坯、工件坐标原点等,建立工件坐标系统,确定工件坐标系与机床坐标系的相对尺寸,从而得到零件加工的正确刀具路径;最后,利用Master CAM系统提供的仿真功能,实现模拟加工。

During maturation, ripening and senescence of tomato and strawberry fruits, calmodulin , a receptor protein of Ca〓, had similar changing trend, but the time when CaM peak appeared was different and related to storage life of fruit. CaM peak in strawberry fruit appeared earlier than in tomato fruit.

进一步研究表明,Ca〓受体蛋白钙调素(Calmodulin,CaM)无论在番茄还是草莓成熟衰老过程中,CaM含量变化均有相似的趋势,但不同果实CaM高峰出现的时间不同,衰老快的草莓果实较衰老慢的番茄果实CaM高峰出现时间早,这与果实的贮藏寿命有关。

TFP activated NAD kinase in tomato and strawberry fruit, CPZ activated the enzyme in tomato but in strawberry fruit, which indicated that ethylene-started and promoted maturation, ripening and senescence of fruit were related not only to increase in intracellular Ca〓 concentration and CaM content, but also to NAD kinase, and indirectly showed that CaM could regulate NAD kinase in two fruit.

以上结果表明乙烯启动和加速果实成熟衰老不仅与胞内Ca〓浓度和CaM含量增加有关,而且与CaM的另一目标酶——NAD激酶有关,并间接证明CaM对两种果实NAD激酶具有调控作用。

Conformation of CaM has changed greatly after bound by Ci, with the result that the binding sites and interaction module can be roughly determined after chemical shifts assignment, perturbation and calculation of KD, which supports the supposal that CaM and CNA C-terminal were in symmetrical homodimer complex2. In addition, the possible position of"NewCBD"in the scale of residue 430-450 and classification of functional domains in C-terminal have also been discussed.

结合了Ci的CaM构象较之于其自由状态发生很大改变,经化学位移指认、微扰实验和解离平衡常数计算后可以初步确定两者的结合位点以及作用模式,支持了CaM与CNA C末端片段以对称的同二聚体形式命名为(CaM·Ci2复合的猜想,推断出"NewCBD"可能存在于430-450区间,对现有的C末端功能区划分作出了一些改进。

During the titration of Ca〓CaM by La〓, the hydrid complex La〓Ca〓CaM forms as the intermediate species. La〓 can bind to calmodulin with high affinity (K〓=10nmol/L) and obvious cooperation between the two global domains. The secondary structure of La〓CaM and Ca〓CaM are highly similar, but there are obvious differences in ternary structures. Excessive La〓 changes the conformation of calmodulin greatly.

实验结果表明,La〓在钙调蛋白上的结合位点与钙离子相同;在结合顺序上La〓以较小的优势先结合在钙调蛋白的N末端;La〓Ca〓CaM是La〓与含钙的钙调蛋白作用时的主要产物;La〓强烈地与钙调蛋白结合,其解离常数约10nmol/L,各个结合位点之间存在明显的协同效应;La〓CaM与Ca〓CaM在二级结构上高度相似,但是在高级结构上有明显差异。

This study consists 3 partsPart I: the research on the mechanism of affecting the growth of CAM vesselsBecause neoformative vessels is most related with bFGF and VEGF, this study designed bFGF, VEGF and the primer of its receptor, by the experiment of the generation of CAM vessels, draw the materials from CAM tissues consist account of vessels, by the experiment of RT-PCR, reserve the influence of HSYA on the expression of bFGF, VEGF, VEGF-R(flt-1) in CAM tissues.

本实验分为三部分:第一部分,HSYA影响鸡胚尿囊膜血管生成的机理研究由于新生血管与bFGF、VEGF及其受体的关系最为密切,本实验设计了bFGF、VEGF及其受体的引物,利用CAM血管生成实验,直接从富含血管的CAM组织上取材,通过RT-PCR实验,观察HSYA对CAM组织中上述三个基因表达的影响。

There are some AChR plaques of morphological abnormality, and no contact sites of N-CAM with AChR although the intensity and scope of N-CAM staining is increased.D: Yiqi Huayu Recipe-treated group on the 20th day after L5 nerve root compression. All the AChR sites are reoccupied by N-CAM and the morphology of AChR are restored. E: Untreated group on the 30th day after lumbar nerve root compression. AChR plaques display irregularity in shape, with a few round or spindle shaped cupulae. Meanwhile, some N-CAM stainings are still shown to be around the AChR. F: Yiqi Huayu Recipe-treated group on the 30th day after L5 nerve root compression.

随着时间的推移,AChR斑的形态渐趋规则,N-CAM反应也逐步增强,至造模后20 d达到最高峰,但益气化瘀方组仍然明显优于模型组,其不仅在数值上超过了胚胎期大鼠,且与AChR全部重叠;而模型组仍可见到不少重叠不全且形态异常的AChR斑。L5神经根受压后30 d,N-CAM的表达水平开始下降,基本局限于终板区内,其下降速度和范围在益气化瘀方组明显大于模型组;模型组不仅N-CAM的表达明显高于益气化瘀方组,且仍能见到一些成熟不全的AChR斑;而益气化瘀方组AChR斑形态已接近于正常。

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It goes back to what I told you...

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With a supporter in Mr Charest and an admirer in Mr Dumont, Mr Harper may be encouraged to call an election himself.

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