查询词典 calli

The results were showed that embryogenic calli were induced from young leaves, which were cultured on MS medium supplemented with 2,4-D 2mg/L and KT 0.2mg/L. For the proliferation of embryogenic calli, the suitable culture medium was MS+BA 8mg/L +NAA 1mg/L. The development and maturation of somatic embryo could be much improved by using the medium of MS+ZT 2mg/L or BA 5mg/L +IAA 0.2mg/L. For the induction of secondary somatic embryo from integral somatic embryo, the suitable culture medium was MS+KT 0.1mg/L+NAA 0.01 mg/L or MS+ZT 0.1mg/L+NAA 0.01 mg/L, the proliferation frequency is 214%, 256% respectively. The cotyledonary generated from somatic embryos of Aesculus hippocastanum L.

本文就欧洲七叶树的组织培养和体细胞胚发生以及植株再生进行了系统研究，主要结果如下：以植物细胞具有全能性的理论为依据，以欧洲七叶树幼嫩叶片为外植体，进行体细胞胚胎发生研究，研究结果表明，诱导愈伤组织的适宜培养基是MS+2，4-D 2mg／L+KT0.2mg／L，MS+BA 8mg／L+NAA 1mg／L有利于胚性愈伤组织的诱导和增殖，添加ZT 2mg／L或BA 5mg／L和IAA 0.2mg／L的MS培养基有利于体细胞胚发育和成熟，体细胞胚可直接诱导次生胚发生，MS+KT 0.1mg／L+NAA 0.01 mg／L或MS+ZT 0.1mg／L+NAA 0.01 mg／L培养基诱导效果最好，增殖频率分别为214％、256％。

1 The assay result of bergenin in calli of nine species of Ardisia A successful system for analyzing content of bergenin in calli of Ardisia spp. was established.

4.1 9个种的愈伤组织中岩白菜素的检测结果通过对文献中实验条件的修改和实验，证明TLC和HPLC法也适用于紫金牛属植物的愈伤组织中岩白菜素的定性、定量分析。

Calli of an indica rice cultivar Qiuguiai 11 were used as material for a further study on the effects of copper in calli cultures.

以籼稻栽培品种秋桂矮11的愈伤组织为材料，对铜元素的作用作了进一步的研究。

Many embryogenic calli were produced through subculture by calli induced from mature embryo of rice.

从水稻成熟胚诱导出的愈伤组织经继代培养后可以产生大量的胚性愈伤组织。

No callus could be induced from leaf or petiole explants of varieties F30 and Ⅱ cultured on MS medium if supplemented alone with 6-bezyladenine （6-BA） or thidiazuron, or kinetin. However, the calli were induced on MS medium containing 2.0 mg L-1 6-BA and 0.5 mg L-1 NAA （α-naphthaleneacetic acid）, and the calli derived from petioles of variety Ⅱ could produce redifferentiated shoots, but of variety F30 could not. The later could only produce shoots directly from the base of the petioles.

将品种F30和Ⅱ的叶片和带叶叶柄接种到含不同浓度单一细胞分裂素（6-BA、TDZ、KT）的MS培养基上培养，均未能诱导出愈伤组织，而在含2.0mgL-1 6-BA＋0.5mgL-1 NAA的培养基上可以诱导出愈伤组织，且由品种Ⅱ叶柄诱导产生的愈伤组织可再分化出芽，品种F30诱导的愈伤组织却不能分化，但在其叶柄基部可直接出芽。

Pale yellow, brown and white translucent calli were produced from zygotic embryos which were stored at 4 to 6℃ for 1 to 3 months and then cultured on medium LP+2mg/L 2, 4-D+1mg/L 6-BA in the dark for one month. Only white trenslucent calli were embryogenic.

合子胚在4～6℃低温条件下保存1～3个月后，接种于LP+2mg/L 2，4-D+1mg/L 6-BA的培养基上，黑暗条件下培养1个月后，产生浅黄色、褐色和白色半透明三种愈伤组织，其中白色半透明愈伤组织是胚性愈伤组织。

In the study 101 local varieties, 83 modern cultivars, 72 new families of wheat were analysed for the days of callusing at 50%, the number of callusing in 4 weeks in callus induction; and rate of Calli of root formation and the rate of calli of shoot formation in differentiation culture.

为了选育商业应用价值好的新品种，作为受体的植物还需要农艺性状好，育种价值高。一个关键问题是，遗传转化技术是建立在植物的细胞和组织通过组织培养过程再生植株的技术体系上。

There is different influence on calli and etiolated seedling under the same red light fluence. This result indicated that rml1 RNA levels in calli and etiolated seedling were regulated by different phytochrome under various light-responsive mechanisms.

在相同的红光流率照射下，愈伤组织和黄花苗表现出不同的红光效应，暗示着不同的光敏色素通过不同的光效应途径调控这两种组织中rml1基因的表达。

The research on the tissue culture and cell suspension culture showed that thesuitable culture medium for inducing bud was MS supplemented with 1.5mg/L 6-BAand 0.1mg/L NAA, and for the generation-continuing multiplication was MSsupplemented with 1.5mg/L 6-BA and 0.2mg/L NAA. The rooting medium was1/2MS supplemented with 0.5mg/L IBA and the rooting rate was 45.0%. Plantletsurvival after transfer to sand was 52.5%.The induction rate of calli was66.7%～86.6% and the optimum medium was MS medium with 0.5mg/L 6-BA and2.0mg/L 2,4-D. The calli became smallest partical size, friable and had gooddispersion ability after 3 times successive transfer culture, the optimum medium wasMS medium with 0.2mg/L 6-BA and 2.0mg/L 2,4-D. Culturing these particles on sixkinds of MS liquid media with different hormone contents, the optimum medium wasselected basing on he change of the density of single-cell, the density of cellaggrefate and the mass of cell.

对蒜头果进行的组织培养与细胞悬浮培养研究结果表明：MS+6-BA1.5mg／L+NAA0.1mg／L+蔗糖3％激素组合能够较好地诱导芽的初始分化和增殖，适宜的芽苗继代增殖培养基为MS+6-BA1.5mg／L+NAA0.2mg／L+蔗糖3％；采用1／2MS+IBA0.5mg／L+蔗糖3％为生根培养基，生根率为45.0％；移栽到河沙的生根苗成活率为52.5％；愈伤组织诱导率为66.7％～86.6％，其中以MS+6-BA0.5mg／L+2，4-D2.0mg／L+蔗糖3％的培养基最佳，其诱导出的愈伤组织具有较强的增殖能力和较好的脆散结构，最佳继代培养基为MS+6-BA0.2mg／L+2，4-D2.0mg／L+蔗糖3％，且培养基中的6-BA与2，4-D浓度的比值越小，愈伤组织生长越快，结构越脆散，增殖率越高；将继代后的愈伤组织转入6种含不同激素浓度组合的MS液体培养基中进行振荡培养，在综合分析各培养基的单细胞密度，细胞团块密度，细胞生物量增长率等指标后，初步筛选出MS+6-BA0.2mg／L+2，4-D2.0mg／L培养基为较好的液体培养基。

When cultured on the medium with different auxin , root segments could elongate and branch, and the addition of 1mg/l IBA into the medium was the most suitable for long-term root culture. Calli could be induced from root explants in the presence of 2, 4-D and when transferred onto E medium, they transformed further into embryonic calli which could develop into normal plants through the stages of globular-embryo, heart-shaped embryo, torpedo-shaped embryo, cotyledonary embryo.

培养基中添加生长激素（IAA，IBA，NAA）能够诱导无菌苗根切段的生长和分枝形成；而1mg／l IBA的存在，最适合于根的长期离体培养。2，4-D的存在能够诱导根外植体脱分化形成愈伤组织，愈伤组织转移至E型分化培养基能够转变成胚性愈伤组织，并进一步经球形胚、心形胚、鱼雷形胚、子叶形胚发育成正常植株。

A historical region of southwest France between the Pyrenees and the Garonne River. The duchy of Aquitaine was joined with France after the marriage of Eleanor of Aquitaine to King Louis VII in1137, but its possession was disputed after her subsequent marriage to Henry II of England.

阿基坦法国西南部一个历史地区，位于比利牛斯山和加隆河之间，在1137年阿基坦的埃莉诺与国王路易斯七世结婚之后阿基坦公国加入法国，但当她再嫁英国亨利二世后，其归属权便受到争议

There are moments when I look at the smiling , happy faces of my expectant staff and wonder how they will cope when confronted by an outraged housemaster along with an unrepentant and recalcitrant bleached blond teenager in 18 years ' time .

有时候，我看着那些满怀期待的员工微笑，幸福的脸，想着当他们遇到一位愤怒的宿舍管理员和一位不知悔改，叛逆，而且将头发染成白色的18岁少年时，他们会如何处理。