查询词典 buffer beam

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

This invention discloses a multi-queue sequence buffer management circuit and a method based on a pipeline applying a pipeline structure including: an arbitration circuit selecting one for process from read, write and distribution buffer requests, a buffer slot state module designing state of the slot requiring operation and queue numbers and assigning idle slots, a buffer slot filter module filtering the slot, a buffer slot filter module filtering the slot states not belonging to the current operation queues nor idle aligned in terms of the head pointer, a queue slot selection module computing continuous idle slot numbers from the slot pointed by the head pointer and refreshing the head pointer and selecting preparing slots, a queue slot prior queuing module refreshing the read pointer and result numbers of the current operation queues with the pointer of the first prepare slots and their numbers which can support multi-queue to share one buffer space, queues can access the buffer in overlap.

本发明公开一种基于流水线的多队列顺序化缓冲管理电路及方法，本发明电路采用流水线结构，包括：仲裁电路，从读、写、分配缓冲请求中选取一路进行处理；缓冲槽口状态设置模块，设置请求操作的槽口状态和队列号，分配空闲槽口；缓冲槽口滤除模块，滤除不属于当前操作队列且非空闲态的槽口状态，按头指针对齐；队列槽口选择模块，计算头指针指向槽口起的连续空闲槽口数并更新头指针，选出预备态的槽口；队列槽口优先排队模块，用第一个预备态槽口的指针和预备态槽口数分别更新当前操作队列的读指针和结果数；本发明可以支持多个队列共享一个缓冲空间，各类指令队列能对缓冲进行交叉访问，并对指令结果的写入读出进行顺序化管理。

Through a combination of T7 RNA polymerase and T7 Promoter for testing proper buffer was selected.The experimental results showed 5×binding-Buffer 1 was more suitable for the test;TBE running-buffer was better than TAE and TGE buffer;0.2×TBE buffer was the best concentration amang there electrophoresis buffer.

通过用T7RNA聚合酶与T7启动子DNA的结合反应来选择合适的缓冲液，结果表明，不同的缓冲液会影响凝胶迁移试验的结果，5×Buffer1结合缓冲液的效果较好；TBE电泳缓冲液比TAE、TGE的电泳效果好，同时在3种不同浓度的TBE缓冲液中，0.2×的TBE效果最好。

Trans-Blot Cells and Systems 170-3825 Trans-Blot Cell With Wire Electrodes and PowerPac HC Power Supply, 100120/220240 V, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm), power supply, power cord, instructions 170-3850 Trans-Blot System With Plate Electrodes and PowerPac HC Power Supply, 100120/220240 V 170-3853 Trans-Blot System With Plate Electrodes, Super Cooling Coil, and PowerPac HC Power Supply, 100120/220240 V, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm), power supply, power cord, instructions 170-3910 Trans-Blot Cell With Wire Electrodes, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) 170-3939 Trans-Blot Cell With Plate Electrodes and Super Cooling Coil, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) 170-3946 Trans-Blot Cell With Plate Electrodes, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) Trans-Blot Cell Accessories 170-3912 Super Cooling Coil, required for all high-intensity transfers 170-3913 Gel Holder Cassette, includes 2 fiber pads 170-3914 Fiber Pads, 15.5 x 20.5 cm, 6 170-3920 Trans-Blot Standard Wire Electrode Card, cathode 170-3921 Trans-Blot Standard Wire Electrode Card, anode 170-3922 Trans-Blot Cell Buffer Tank 170-3923 Trans-Blot Cell Lid With Power Cables 170-3943 Trans-Blot Platinum Anode Plate Electrode 170-3944 Trans-Blot Stainless-Steel Cathode Plate Electrode 170-3945 Trans-Blot Plate Electrode Pair, platinum anode and stainless-steel cathode 16 规格:说明: Trans-Blot Plus

电泳转印槽组件 1。缓冲液槽及带有电缆的盖 2。凝胶支架转印夹 3。纤维衬垫 4。电极丝 5。电极板 6。特级冷却芯 Trans-Blot 转印槽是功能灵活的转印设备，可理想地用于多种转印应用。Trans-Blot 转印槽特点包括：*能进行多胶转印，可容纳3 块PROTEAN I xi 凝胶、6块Criterion 凝胶、12块Mini-PROTEAN 3 或Ready Gel 预制胶*多组参数灵活可设，可调节的电压设置（从30 V 的过夜转印到200 V 的1 小时快速实验）*电极间距设置为8 cm 用于标准印迹杂交，或设置为4cm 用于高强度印迹杂交*可选择板式电极：涂有铂金的钛作为正极，不锈钢为负极，能提供高强度电场和比其它电极更高的电流密度。或选择较经济的铂金电极丝*通过特级冷却芯和水循环仪来调节温度―是天然酶（4°C）或高强度转印的理想选择，随着转印时间增加（多达24 小时），不会引起缓冲液耗竭（在高强度转印中必须使用冷却芯，也推荐用于所有板式电极的应用）*带铰链的凝胶支架转印夹能避免滑动，确保凝胶与印迹膜间的紧密接触；每个转印夹都有颜色标记以保证在转印槽中的正确定位 Trans-Blot 转印槽的锁闭凝胶支架转印夹系统。转印夹（1）支撑凝胶（2）印迹膜（3）两侧有纤维衬垫和滤纸（4）确保凝胶三明治内的完全接触。凝胶夹垂直插入缓冲液槽中（5）。

The arch bending on the top of the original main arch ring corresponds to every arch rib and the bottom beam on its landscape orientation. As a result it becomes a down arc frame similar to slab-and-beam floor system. On the arch bending and bottom beam just established, standing pillar, main beam, horizontal rib beam and bridge front-panel would be built subsequently. Both ends of the main beam would then be put off the new hat beam made of reinforced concrete. Now, it becomes an upper slab-and-beam floor system. Two decks will be joined together on the middle of the bridge, which forms the ability of spanning and loading.

在原主拱圈拱肋相应位置处增设拱伏，横向增设底梁，形成一个下层弧形的类似肋梁楼盖的结构；在已浇筑好的拱伏与底梁上，继续现浇立柱、主梁和横向肋梁、桥面板，主梁的两端搁置在桥台处新增加的钢筋混凝土台帽梁上，形成一个上层肋梁楼盖结构，并且上下两层楼盖在桥梁跨中互为渗透结合成一个牢固的整体，共同完成了跨越和承载的能力。

This paper proceeded the flexural experiment respectively of two flexural strengthen beam by CFS, one compared reinforced concrete beam, and proceed sheared experiment research of two U style stirrups of CFS combined compacted sheared strengthen beam, one compared reinforced concrete beam. analyze and compare some working characters such as width of cracks, the deflection and ultimate moment of an unstrengthen compared beam, an initial strengthen beam and a beam strengthened after crack by compacting .

本文分别进行了2根粘贴碳纤维布受弯加固梁和1根钢筋混凝土对照梁的受弯试验研究及2根碳纤维布U形箍+压条受剪加固梁和1根钢筋混凝土对照梁的受剪试验研究，分析对比了对照梁、直接加固梁、压裂卸荷后再加固梁三者的挠度、裂缝、极限荷载等工作性能。

Secondly, a theoretical dual rigid-plastic modal under lateral impact of blunt indenters is firstly developed in order to predict damage pipeline which is fully clamped across a span based on rigid-plastic beam on a perfectly plastic foundation, which is made up of a rigid-plastic sub-beam and a rigid-plastic basic-beam, the rigid-plastic sub-beam is used to predict denting deformation, the rigid-plastic basic-beam is used to predict global deformation, the sub-beam and the basic-beam interact by a perfectly plastic foundation.

为了更好地估计钝头弹体冲击对管道造成损伤，在刚塑性地基梁模型的基础上，首先提出了一种新的理论计算模型一双重刚塑性梁模型，由一子梁和一基梁构成，子梁用来估计弹体撞击造成的局部损伤，基梁用来估计弹体撞击造成的整体弯曲，子梁和基梁通过基础反力相互作用。

This invention relates to a method and device for generating cold atomic beam. Said method includes heating hot atom source in vacuum chamber to form atomic saturated vapor pressure atmosphere, cooling hot atom by three-D MOT to below 200uk and trapped to form cold atomic cloud, by the quarter wave plate reflection mirror in MOT the laser radiation pressure being unbalanced to make cold atom emitting along said direction, four tape arranged straight line set with contrary current direction is set in atom beam emission direction, which makes the atom emitted to forward to obtain cold atom beam with low speed, large flux and small transverse speed, laser beam vertical with atom beam set in the direction of cold atom emitting for atom beam state preparation to realize state concordant emitted cold atom beam.

本发明涉及一种冷原子束产生方法和装置，该方法包括在抽真空的真空室内，加热热原子源，在真空室中形成相应的原子饱和蒸汽压气氛；采用三维MOT对热原子进行冷却，冷却到200μk以下，并被捕获形成冷原子云团；通过三维MOT中开小孔的四分之一波片反射镜，而使得在该方向上激光辐射压力不平衡，使冷原子沿此方向出射；在冷原子束出射方向上，还置有载有彼此电流方向相反的四根成锥形排列的直导线，从而原子出射时将在磁场的作用下，不会沿横向膨胀而向前射出；便得到了出射速度低的、通量较大的、横向速度非常小的连续冷原子束；同时在冷原子束的出射方向上，设置有与原子束垂直的激光束，用于原子束的态制备，实现态一致的出射冷原子束。

The invention discloses a LCD panel, colorful filter piece and making method, which comprises the following steps: providing a base; forming a buffer material layer on the base; forming patterned lightproof layer on the buffer material layer; adopting patterned lightproof layer as lid to pattern the buffer material layer to form a patterned buffer layer; forming a separating piece through patterned lightproof layer and patterned buffer layer; making the separating piece form multiple pixel areas on the base; forming a colorful filter layer in each pixel area.

本发明公开了一种液晶显示面板、彩色滤光片及其制作方法，其中彩色滤光片的制作方法包括下列步骤：首先，提供一基板，并在基板上形成一缓冲材料层。接着在缓冲材料层上形成一图案化遮光层。以图案化遮光层为罩幕来图案化缓冲材料层而形成一图案化缓冲层，使得该图案化遮光层与图案化缓冲层共同形成一隔离件，且隔离件在基板上形成多个像素区域。然后在每一像素区域内分别形成一彩色滤光层。

Based on some related concepts of shock disturbed system, weakening buffer operators with variable weights and strengthening ones with variable weights are established respectively. This subject defines buffer operator regulation degree which reflects effect strength to original sequence caused by buffer operator, and also studie the relationship between buffer operator regulation degree and variable weight. Consequently, the monotonicity nonvariable axim of buffer operator is put forward.

基于冲击扰动系统的相关概念，提出了变权缓冲算子的概念，分别构造了变权弱化缓冲算子和变权强化缓冲算子，定义了缓冲算子调节度来反映缓冲算子对原始序列的作用强度，并研究了缓冲算子调节度与可变权重之间的关系，在此基础上提出了缓冲算子的单调性不变公理。

The company mainly produces all kinds of industry screening machines, food packaging machine (solid, liquid, granular, powder), and bottled beer packaging machine assembly line, as well as large-scale wind transport, dedusting equipment design and installation.

公司主要生产各种行业用筛选机、食品包装机（固体、液体、粒状、粉状）、啤酒包装机和罐装流水线以及大型风力输送、除尘设备的设计与安装。

They began to embroider little squares of cloth.

陪同他的是学校的校长，西科尔斯卡小姐。