查询词典 blot up

The expression level of metabolitic enzyme (CYP1A1, CYP1B1) and its correlation with cell differentiation /apoptosis were studied as well. The influence of resveratrol in cell cycle was determined with FCM. Results 1 Resveratrol not only suppresses the growth of medulloblastoma cells in a dose and time related fashion but also induces cell differentiation and apoptosis. Resveratrol promotes differention of UW228-1,-2 cells to forward glia, UW228-3 and Med-3 cells to neuron. A treatment of resveratrol in the concentration of 100μM for 48hrs comit most of cells die of apoptosis. 2 Among the four cell lines Fas and Caspase-3 were constantly expessed, whereas FasL was absent irrespective to the drug treatment. 3 ICC, RT-PCR, Western-blot hybridization and EROD enzymatic activity assay demonstrated that expression of CYP1A1 is different after treatment with resveratrol in four cell lines, up-regulated in three UW228 cell lines in a dose dependent manner but down-regulated in Med-3 cells. Suppressive effect of resveratrol on CYP1B1 expression is the same among four medulloblatoma cell lines. 4 Cell cycle distribution of UW228-3 was greatly changed after treatment.

结果：1、白藜芦醇以时间和剂量依赖性方式抑制髓母细胞瘤细胞生长，同时促进细胞的分化和凋亡：白藜芦醇促使UW228-1、-2靶细胞向神经胶质细胞方向成熟分化，UW228-3、Med-3细胞向神经元细胞方向成熟分化，以100μM作用48小时效果显著；2、四个细胞系均有Fas和Caspase-3表达，但无Fas-L基因表达和蛋白活性产生，因此难以形成Fas相关性死亡通路；3、白藜芦醇处理前后CYP1A1基因的表达在各细胞系略有不同：UW228-1，-2，-3三个细胞系白藜芦醇以剂量相关性方式上调CYP1A1的表达，而Med-3细胞系，白藜芦醇却抑制CYP1A1的表达；四个细胞系在白藜芦醇处理后CYP1B1的表达均受到抑制；4、UW228-3细胞系经白藜芦醇作用后细胞周期有较大改变，表现在G0/G1期在整个细胞周期中所占的比例增加，而其它时期则相应减少。

Calcium sulfate has been used for bone regenation in oral medicine. Osteopontin, a marker molecule, expressed by osteoblast, plays multiple roles in human body. However, the effect of calcium sulfate on osteopontin expressed in osteoblasts is unclear. In this study, U-2 osteosarcoma osteoblastic cells were cultured in 0, 0.5, 1, or 10 μM calcium sulfate hemihydrate. There was no effect in the proliferation of cells, suggesting that calcium sulfate up to 10 μM is biocompatible. The cells and conditioned media were collected for analyzing mRNA and protein levels of osteopontin by reverse-transcription polymerase chain reaction and Western blot, respectively.

硫酸钙用於口腔医学中的骨头再生已经有多年历史，骨桥素是一种被骨母细胞分泌且在人体扮演重要多重角色的标的分子，然而，硫酸钙在骨母细胞分泌硫酸钙上的影响仍未明朗，在本实验之中，培养U-2骨肉瘤类骨母细胞(U-2 osteosarcoma osteoblastic cells)於0、0.5、1、10 μM的硫酸钙之中，在细胞数目的复制上，四种硫酸钙浓度并无影响，显示出U-2骨肉瘤类骨母细胞培养於10 μM的硫酸钙仍是生物相容性的。

Make-work schemes mop up some of the jobless, but may blot a curriculum vitae.

化妆的工作解决了一些人的待业问题，但是这并不需要学术的简历。

Cities and meet and discuss of royal this pair of old enemy are sure the battle that is a life and death, common saying says: Personal enemy meets especially jealous, royal the trouble that this 9 cities bring accord can be not small really, in the move that absorbs wildly the user of 9 cities does not say, its publicized strength to had arrived wildly inconceivable degree, from the Jin Mao of Shanghai the edifice arrives blot out the sky and cover up the earth network advertisement, publicize to the sundry activity in game again.

九城和盛大的这对老冤家碰头必定是一场生死之战，俗话说：仇人见面分外眼红，盛大这次给与九城带来的麻烦可真是不小，在疯狂吸收的着九城的用户不说，其宣传力度已经疯狂到了不可想象的地步，从上海的金茂大厦到铺天盖地网络广告，再到游戏中各式各样的活动宣传。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

Last-minute, support searchs engine and live, the search engine regards as the stationmaster of parents of him back and belly, it is bubble is in free marsh cannot the person of extricate oneself, discharge wants free, the program wants free, the domain name also had better be free, should talk only receive fund, the fall out on the horse, but more acid is, want to search engine to change a bit a little only, or advertisement alliance money gives little, the clapperclaw that blot out the sky and cover up the earth began, as if the whole world is right only he is one.

归根究底，依靠搜索引擎而活，将搜索引擎当作自己衣食父母的站长，都是泡在免费沼泽里不能自拔的人，流量要免费的，程序要免费的，域名最好也是免费的，只要谈收钱，马上翻脸，但是更加讽刺的是，只要搜索引擎稍微有点变化，或者广告联盟钱给少了，铺天盖地的痛骂就开始了，仿佛全世界对的只有他一个。

RESULTS:①Westem blot of the tendons in experiment group demonstrated the up-regulation of TGF-β receptor protein levels, which mainly appeared in the tendon sheath, the epitendineum and the incision along the repair site.

肌腱损伤修复后，肌腱和腱鞘转化生长因子β1受体的表达明显增加，在术后14d达到高峰，56d开始降低，这种受体上调可能为屈指肌腱术后瘢痕形成的生物学调节提供新的途径。

To elucidate the molecular mechanisms underlying the neural plasticity after CNS injury, the gene expression profile in the rat hippocampus following perforant path transections was explored by several methods including custom differential screening, custom cDNA array and cDNA microarray. Of 2300 cDNA clones from low-abundance rat brain cDNA bank, 6 were identified as differentially expressed genes/ESTs in the hippocampus 35 days after lesion, in which none was confirmed by Northern blot. Of 8000 cDNA elements, custom cDNA array identified 47 as differentially expressed genes/ESTs with above 3-fold changes in the hippocampus 35 days after lesion, in which 25 were up-regulated and 22 down-regulated.

中文题名去传入海马的差异表达基因筛选和Cystatin C表达副题名外文题名 Screening of the differentially expressed genes and cystatin C expression in the deafferented hippocampus 论文作者应国新导师周长福研究员学科专业神经生物学研究领域\研究方向学位级别博士学位授予单位中国科学院上海生命科学研究院学位授予日期2002 论文页码总数121页关键词海马星型胶质细胞 Cystatin C 基因表达去神经海马馆藏号BSLW /2003 /Q42 /27 为了阐明中枢神经系统损伤后神经可塑性的分子机制，本实验探索了多种方法，包括传统差异筛库、传统cDNA array和cDNA microarray，对穿通纤维切断后大鼠海马的基因表达图谱进行了研究。

Call its " stunner " not beyond the mark, the propagandist offensive that blot out the sky and cover up the earth, the belle is horizontal old advertisement, be without the game kernel of connotation, the game that wants money indefinitely is substaintial, the game kind that allows this to be valued by industry height originally turned a vase that only appearance is without connotation into the decoration, and because webpage game is in,face test of life and death when, even more fling caution to the winds to make exterior text wantonly, scene of even more low vulgarization, love changes the impression that brings about webpage game to give a person, also become game industry to be regarded as the fuse of an oaf event that the industry does not have culture finally.

称其为&尤物&一点不过分，铺天盖地的宣传攻势，美女横陈的宣传广告，毫无内涵的游戏内核，无限要钱的游戏本质，让这个原本被业界高度看好的游戏类型变成了一个只有外表毫无内涵的花瓶摆设，而由于网页游戏在面临生死考验之时，越发的不顾一切大肆做表面文章，导致网页游戏给人的印象越发低俗化、情色化，也成为游戏产业最终被视为一个有产业无文化的畸形儿事件的导火索。

Fortunately, the prisoners did not wake up tomorrow a strong responsibility to hold possession, but after weighing the pros to thank him and let him have a reason to punish should have, both with each other when they meet again, said with regret, is willing to bear all of the results, This more or less eliminates the tubes out strong negative impact of the unit, Meng-Tsan Chiang had been staying during the observation accompanied by his side, and she said that the experience of his own daughter grow up, easy to deal with the problem is almost evening look, see Meng-Tsan Chiang not to return to En Yu's intention and asked if she could make Li Xing Yang months and come back to take in order to blot Fortunately, her childhood years to save some special scenarios, winter vacation has just come, I heard that Li Xing see his uncle to bring his mother, Yang months are excited, and bags large and small to pack a lot of things, is like home for Spring Festival is not the general, Qi-line anomalies that Meng-Tsan Chiang's trip goes well, hope she will take the opportunity to go out a chance to get a rest, if the Yang on call around, do not worry too much too tired, know that it is easy to Xi's idea, plus there are plum star escort, they did not say much else, then, and chose to send two men to the station early morning, because in a hurry to go to work, no more leave time to say good-bye to the unit.

幸运的是醒来后的犯人并未追究管明强的责任，痛定思痛之后反而感谢他，让他得到了理应该有的惩罚，两人再次见面时都互相表示着抱歉，愿意承担所有的结果，这多少消除了管明强在单位的负面影响，留守观察的期间孟灿一直陪在他身边，和她说自己女儿成长的经历，易夕看问题处理差不多了，见孟灿还没有要回恩宇的打算，便问她可否让李子星带杨晓月过来看看，以好在她童年的印迹里保存一些特别的情景，寒假刚刚来临，听说李子星叔叔要带自己见妈妈，杨晓月异常兴奋，大包小包收拾一大堆东西，好似春节也不回家过的般，杨启行得知孟灿此行异常顺利，希望她趁外出的机会好好休息休息，如果将杨晓月叫身边，也不要太操心太累，得知是易夕的主意，加上还有李子星护航，便没有多说别的话，择日清晨送两人去了车站，因赶时间上班，没多留点时间就道别去了单位。

The big eared hedgehog, the little owl, the sand gecko, the three-toed jerboa, and every show needs a villain - saw-scale - the viper!

大耳刺猬，小猫头鹰，沙壁虎，三趾跳鼠，每场戏都需要反派角色：蝰蛇。

Plasmodiophora brassicae ; resting spores ; density gradient centrifugation of sucrose ; ultrasonic disruption

根肿；休眠孢子；蔗糖密度梯度离心；超声波破碎