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Therefore, the appropriate concentration of Arctium lappa L. extract was 30 mg/L when considering spinach's growth, quality and the cost of Arctium lappa L. extract.

因此,从对菠菜的生长情况、品质情况及经济角度综合考虑,牛蒡提取液应用在菠菜上的适宜浓度为30 mg/L。

Object To explore the method for high quality DNA extract from plant Chinese medicinal materials containing developed xylem Methods The process of DNA includes wash with Tris buffer, extract with CYAB buffer and purification mainly Results Using this method, the total DNA was isolated from Cimicifugae foelida L., Arlia chinensis L., Paeoniae lactiflora Pall.,Aucklandia lappa Decne.,Lindera aggregata Kostern., and Akebia quinata Decne.

目的 为获得高质量的DNA,研究木质部较发达的根,根茎类以及茎木类药材的DNA提取方法。方法总DNA的提取过程包括用Tris缓冲液洗净,CTAB抽提和纯化。

Both 50% boiling water extract and 50% alcohol extract of Zingiber Coral- linum Hance do not inhibtte the five tested bactreial species:Staphy.aureus, Strept.hemolytieus,B.subtilis,E.coli and S.typhus.

本文报告对珊瑚姜抑制细菌和真菌作用的研究。结果表明珊瑚姜50%水煎剂和50%酒精浸液对金黄色葡萄球菌、乙型溶血性链球菌、枯草杆菌、大肠杆菌和伤寒杆菌都无抑制作用。

Results calf liver extract at 100 mg/l could protect the hepatocytes from injury, but the effect was not obvious. at 500 mg/l, calf liver extract could protect the hepatocytes obviously. with increasing concentration, the protective effect weakened gradually.

结果 小牛肝提取物在100 mg/l时即可起到保护肝细胞损伤的作用,但效果不明显,在500 mg/l时可明显保护肝细胞损伤,之后随着浓度的加大保护作用逐渐减弱。

Methods hepatocytes were digested by iv-collagenase and cultured with low-serum medium, induced by h2o2 after 12~16 h. various concentrations of calf liver extract were added to the primary cultured hepatocytes to observe the effect of (100~1 000) mg/l calf liver extract on the injured liver cell.

采用ⅳ型胶原酶消化法制备游离肝细胞悬液,以低血清进行肝细胞原代培养,12~16 h后用0.1 mmol/l的h2o2诱导肝细胞损伤,同时加以不同浓度的肝提取物,观察(100~1 000)mg/l的小牛肝提取物对肝细胞损伤的影响。

Representation of the world, mainly in the placental extract, Switzerland and Japan Placenta with a high purity, high-quality and high security features, extracted from the cells, the completion of injection can be divided into three stages: First, using the most advanced chemical and biological equipment, from the sheep embryo to extract the liver activity of the protein to remove impurities, heat-sensitive protein and can cause allergic reactions of the various types of risk factors, followed by the adoption of advanced technology on the extraction of viable cells were isolated, and thus be the activity of cells with different efficacy, such as the thymus cells, placental cells, liver cells and so on dozens of different types, the final freeze-dried using the most advanced technology to help keep cells alive nature.

胎盘素具有高纯度、高品质和高安全性的特点,从细胞的提取到针剂的完成可分为三个阶段:首先运用最先进的生化仪器,从小羊胚胎的肝脏中提取活性蛋白质去除杂质、热敏感蛋白质及可能导致过敏反应的各类危险因子,其次通过先进的技术对提取的活性细胞进行分离,从而得到具有不同功效的活性细胞,如胸腺细胞、胎盘细胞、肝脏细胞等几十种不同类型,最后采用目前最先进的冻干技术来保持细胞鲜活性。

The crude extract rate was accounted for 2.62%. The antilipid-peroxidation of the extract on liver was assayed by the determination of malondialdehyde with TBA spectrometric method.

用分光光度法测定了芝麻饼粕提取物对DPPH的清除作用和对小鼠离体组织匀浆脂质过氧化及Fe(上标 2+)-VC体系诱导的脂质过氧化的抑制作用。

Calf liver extract could protect hepatocytes from damage induced by H2O2, the effect at a concentration of 500 mg/L was the most obvious.Key words:calf liver extract; H2O2; hepatocytes culture; hepatocytes injured; protective effect

小牛肝提取物对H2O2诱导的肝细胞损伤具有一定的保护作用,其中以浓度为500 mg/L时效果最明显。

Among the carbon sources examined, brown sugar and malt extract were best for the mycelial growth and the polysaccharide production. Besides, yeast extract and skim milk were the effective nitrogen sources.

在所测试的数种碳源中,以brown sugar和malt extract对菌丝生长及多醣生产效果较佳。yeast extract 和 skim milk为较佳之氮源。

METHOD Two in vitro experiments were performed for comparative study to evaluate the effect of extract, namely, adapting the addition of the contained medicine serum to culture medium and adding extract to the medium directly.

采用两种离体方法对比观察,即以家兔灌服该提取物后获取的含药物血清加至细胞培养基中的血清药理学方法和将提取物直接加至培养基中的直接体外给药法。

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马哈拉施特拉邦内政部高级官员Pradeep Indulkar称,有101人在冲突中丧生,287人受伤。

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