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The invention provides a method for producing transgene sugarcane quickly with pmi genes. The method primarily comprises the steps of establishing a mannitose screening system; transformation of a gene gun; acquisition of resistant callus; differentiation and screening of the resistant callus; acquisition and rootage of resistant plantlets; molecule detection of resistant plantlets regenerated and detection of pmi gene expression by the chlorophenol red detection method.
一种利用pmi基因快速获得转基因甘蔗的方法,主要内容包括:甘露糖筛选体系的建立,基因枪转化和抗性愈伤组织的获得,抗性愈伤组织的分化筛选,抗性苗的获得和生根,抗性再生苗的分子检测和氯酚红法检测法检测pmi基因表达。
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Results: The proportion of Gram-negative bacilli rose obviously, so did the opportunistic pathogen's and Yeasts detected rate. Most drug sensitive tests showed the drug-fast rate turned to be high. Escherichia coli, Salmonella and Citrobacter resistant and so on to fluoroquinolones was 57%~97%. These bacteria to imipenem showed excellent activity. Meticillin-resistant-staphylococcus resistant to ciprofloxacin, norfloxacin, clindamycin, tetracycline, erythromycin, cefazolin and ampicillin were 40.9%, 42.4%, 63.6%, 72.2%, 85.9%, 100.0% and 100.0%, respectively Streptococcus and Enterococcus resistant to major β-lactams-penicillin, aminoglycosides, macrolides, tetracyclines and sulfonamides were between 36.0% to 100.0%.
结果:革兰阴性杆菌比例显著升高,条件致病菌和酵母样真菌检出机会显著增多;药敏结果显示多数临床细菌对常用抗生素的耐药趋势在不断上升;大肠埃希菌、沙门菌和枸橼酸杆菌等革兰阴性杆菌对喹诺酮类药物的耐药率达57%~97%,亚胺培南则对阴性杆菌有较高的敏感覆盖率;耐甲氧西林葡萄球菌对环丙沙星、诺氟沙星、氯洁霉素、四环素、红霉素、头孢唑啉和氨苄西林的耐药率分别为40.9%、42.4%、63.6%、70.2%、85.9%、100.0%和100.0%;链球菌和肠球菌对多数β内酰胺类青霉素、氨基糖苷类、大环内酯类、四环素类以及磺胺类药物均呈高度耐药,耐药率在36%~100%。
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The key products of the company are high alumina brick ,fireclay brick ,phohate-bonded wearable brick,exfoliation-resistant high alumina brick,High refractorine under load brick,corundum brick,magnesite brick,magnesite chrome brick,series of corundum,SIC furnace chamber and pipe , ceramisite brick, corundum-mullite brick,corundum-chrome brick,sillimanite brick,magnesite alumina inel brick,acid-resistant brick,alkali-resistant brick , zirconia-corundum brick , alumina bule products, series thermal iulating refractory products ,high alumina castable,corundum castable ,corundum ramming ,brown-corundunm castable ,SiC castable ,low-cement castable ,alkali resistant castable ,Light silica alumina products, stainle steel fibre reinforced castable ,etc.
主要产品有:高铝质砖、粘土质砖、磷酸盐耐磨砖、抗剥落砖、高荷软砖、刚玉砖、碳化硅砖、镁砖、镁铬砖、各种刚玉、SIC炉膛、炉管制品、陶粒砖、刚玉莫来石砖、铬刚玉砖、硅线石砖、镁铬尖晶石砖、耐酸砖、耐碱砖、锆刚玉砖、氧化铝空心球制品及各种轻质保温材料、高铝质浇注料和刚玉浇注料、捣打料、棕刚玉浇注料、碳化硅浇注料,低水泥浇注料、抗碱性浇注料、轻质硅酸铝制品、钢纤维增强浇注料等80余种系列耐火材料。
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The antibiotic resistance of staphylococcus aureus was detected by K-B method. Of 33 staphylococcus aureus stains isolated from food, 28 were resistant to penicillium and ampicillin, the resistance rate was as high as 84.8%.21 stains were resistant to carbenicillin, the rate was 63.6%.5 stains were detected resistant to methicillin, which took up to 15.2%. The stains also were resistant to cephalosporin antibiotics, aminoglycoside, microcline antibiotic and tetracyclines in different levels, all strains were sensitive to vancomycin.
结果 33株食品中分离的金黄色葡萄球菌,有28株对青霉素和氨苄西林耐药,耐药率高达84.8%;21株对卡比西林耐药,耐药率达63.6%;检出了5株耐甲氧西林的菌株,耐药率为15.2%;对其他头孢类抗生素、氨基糖苷类、大环内脂类、四环素类等都出现出不同程度的耐药;所有菌株均对万古霉素敏感。
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For customers inside and outside the province for transport; by special quality, I plant dealers and users throughout the country; to telephone to announce the minimum price; 2, ultra low-cost quality products types: professional production : thousands of color paint, silver paint, blending paint, alkyd paints, Nitro-paints, silicone paints, acrylic paints, Antirust Paint, crossed the road paint, heat-resistant high temperature paint, works with paint, fast Do paint, whiteá, lead oil, perchlorethylene paint, epoxy paint, polyurethane paint, alkyd enamel, bitumen paint, perchlorethylene paint, epoxy paint, epoxy silicone high-temperature paint, epoxy coal tar pitch paint, epoxy zinc-rich primer, silicone high temperature anti-corrosion paint, matt paint, matt paint, steel with paint, colored perchlorethylene anticorrosive paint, Quick-drying silver paint, quick-drying silver paint, rust-proof enamel silver, silver Pulp Antirust Enamel, Silver Enamel, Silver paint, silver aluminum paint, aluminum paste paint, aluminum paint, aluminum paste, silver, acrylic paint works, acrylic paint machinery, acrylic anti-corrosion paint, acrylic road is zoned line of paint, quick-drying Antirust Paint, Rusted Antirust Paint, anti-rust primer, black Antirust Paint, white Antirust Paint, Iron Oxide Red Antirust Paint, Medium Gray Antirust Paint, gray Antirust Paint, Anti-Hongdan rust paint, primer, paint, enamel, works with paint, iron and steel works with paint, floor paint, road sign paint calculus, community signs paint, paint Cell lines, highways crossed paint, enamel nitro, nitro topcoat, primer nitro, nitro external enamel, Enamel with a nitro, nitro-iron paint, nitro Wood, nitro varnish, high temperature silver paint, high temperature Antirust Paint, high temperature resistant silicone paint, organic silicon heat-resistant paint, heat-resistant high temperature paint, steel paint, varnish Lek, Lek coal epoxy varnish, epoxy coal pitch antiseptic paint, alkyd paints to reconcile, Blending alkyd paint, fast drying alkyd paint, alkyd Enamel, mechanical equipment paint, paintê,·ásteel, steel anti-corrosion paint, perchlorethylene paint, epoxy rusted Antirust Paint, Epoxy Floor paint, acrylic paint, acrylic paint crossed the road, crossed the road was drying paints, insulating paint, since the insulation do paint, paint the chimney, chimney Antirust Paint chimney anticorrosive paint, paint thinner, special paint thinner, the factory price is lower than the other plant 1 / 2; hundreds of paint quality, durable, high quality and low price, by the authority of certification, the quality of comprehensive standards, and provide product specification; bucket can also be the lowest shipping bucket can also be customized!
可为省内外客户办理运输;靠优质特价,我厂经销商和用户遍布全国各地;来电话即可告之最低价格;,超低价的优质产品种类:★专业生产:数千种色油漆,银粉漆,调合漆,醇酸油漆,硝基油漆,有机硅漆,丙烯酸漆,防锈漆,马路划线漆,耐热耐高温漆,工程用漆,快干油漆,白厚漆,铅油,过氯乙烯漆,环氧漆,聚氨酯漆,醇酸磁漆,沥青漆,过氯乙烯漆,环氧树脂漆,环氧有机硅高温漆,环氧煤沥青漆,环氧富锌底漆,有机硅高温防腐漆,亚光漆,无光漆,钢铁用漆,各色过氯乙烯防腐漆,速干银粉漆,快干银粉漆,银粉防锈磁漆,银浆防锈磁漆,银粉磁漆,银浆漆,铝银粉漆,铝银浆漆,铝粉漆,铝银浆,银粉,丙烯酸工程漆,丙烯酸机械漆,丙烯酸防腐漆,丙烯酸马路划线漆,快干防锈漆,带锈防锈漆,防锈底漆,黑防锈漆,白防锈漆,铁红防锈漆,中灰防锈漆,灰防锈漆,红丹防锈漆,底漆,面漆,磁漆,建筑工程用漆,钢铁工程用漆,地坪漆,路牙石标志漆,小区标志漆,小区划线漆,公路划线漆,硝基磁漆,硝基面漆,硝基底漆,硝基外用磁漆,硝基内用磁漆,硝基铁器漆,硝基木器漆,硝基清漆,耐高温银粉漆,耐高温防锈漆,有机硅耐高温漆,有机硅耐热漆,耐热耐高温油漆,钢结构漆,沥清漆,环氧煤沥清漆,环氧煤沥青防腐漆,醇酸调和油漆,醇酸调合油漆,醇酸快干油漆,醇酸磁漆,机械设备漆,玛钢漆,钢结构防火漆,钢结构防腐漆,过氯乙烯漆,环氧带锈防锈漆,,环氧地坪漆,丙烯酸漆,丙烯酸马路划线漆,快干马路划线漆,绝缘漆,自干绝缘漆,烟囱漆,烟囱防锈漆,烟囱防腐漆,油漆稀释剂,特种油漆稀释剂,本厂家价格低于其它厂1/2 ;数百种油漆质量第一,经久耐用,质优价廉,通过权威认证,质量全面达标,并提供产品说明书;一桶也可以最低价发货,一桶也可订做!
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We selected mecA gene and femA factor asβ-lactam antibiotic-resistant genes, ermA, ermC, msrAgene as macrolides-lincosamids antibiotic-resistant genes, norA, grlA, gyrA, gene as Quinolonesantibiotic-resistant genes, and tetM gene as Tetracycline antibiotic-resistant genes on the basis of the latestresearch. We designed primers with GenBank enunciable gene sequence, cloned the drug resistantgenes, and sequenced these genes.
根据国内外最新文献报道,筛选出对β-内酰胺类抗生素耐药的mecA基因及辅助因子femA;对大环内酯类及林可霉素类抗生素耐药的ermA、ermC、msrA基因,对喹诺酮类抗菌药物耐药的norA、grlA和gyrA基因及对四环素类抗生素耐药的tetM基因作为拟选的主要耐药基因,分别设计PCR扩增的特异性引物,进行克隆。
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The results showed that resistant gene Ht in F1 heterozygote was the most effective on controlling spots number and size; all monogene in heterozygote could produce spore by moisture culture and resistant gene Ht1 was the most effective; resistant gene Ht1 in medium level, Ht3 in high level, and Ht in low level of vertical resistance of heterozygote, were the most effective on controlling spots spread; while Ht2 in high level of vertical resistance of heterozygote was the most ineffective on controlling spots spread; and all resistant monogene in heterozygote showed superior to their parents on controlling incubation period and gene Ht was more significant than other genes.
结果表明,抗性单基因Ht处于F1代杂合状态时,控制病斑数和病斑面积的效应最强;单基因处于杂合状态时,湿培48h病斑全部能产生分生孢子,且处于同一水平抗性杂合状态时,Ht1控制产孢量的效应最强;Ht1处于中水平、Ht3处于高水平和Ht、处于低水平垂直抗性杂合状态下,控制病斑扩展效应最强;而Ht2处于高水平垂直抗性杂合状态下,控制病斑扩展效应最弱;抗性单基因处于杂合状态下,控制潜伏期多表现超亲现象,Ht控制病害潜伏期比其它单基因显著。
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UCB01S is of oil resistant,acid resistant,alkaliresistant and seawater resistant.more over,there is no lead in the PTFE surface layer and so is particularly fit for bushings in food stuff machines,alkali flow meters,pumps motion elements in pharmaceutical machines,printing machines chemical engineering machines and other ocean industry.this is a triple layers composites bush,the base material being a bronze plate and a film of heat resistant power filled PTFE being calendered onto the sintered spherical bronze interlayer.
UCB01S不锈钢耐腐蚀轴承,是以不锈钢材料为基体,中间烧结耐腐蚀合金粉末,表面轧制以聚四氟乙烯为主的低摩擦材料,经过卷制成型的一种十分有效的耐腐蚀材料。它具有耐油、耐酸、耐碱、耐海水和耐磨损的特点,表面的PTEE材料不含铅成份。在食品饮料机械、印染机械、化工机械、海洋工业耐腐蚀滑动部位最适合使用。
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In this study, the author used GISH technology to analyze the material that was resistant to take-all in test, and got three chromosome arm addition lines that carried the resistance genes derived from the amphiploid of Triticum-Haynaldia. The above arm addition lines and their resistant and sensitive parents were analyzed by RAPD method and two specific molecular markers that were tightly linked to resistant gene were obtained.
本研究先用基因组原位杂交对经鉴定抗全蚀病的材料进行分析,检测出三个抗性源于簇毛麦的端体异附加系材料,再利用RAPD方法,对这三个材料和抗感病亲本进行分析,得到了两个与全蚀病抗性基因紧密相关的特异性RAPD标记。
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Material has low carbon steel A3, 45 # steel, ICY13 heat-resistant steel, ICY18N19Ti steel, oocr18Ni14MO2Cu2 acid-resistant heat-resistant steel and so on. 2, food cookies Industry: Width 180mm-1200mm cotton, nylon line canvas belt, mesh belt ovens, cooling injector machine with Net, Net-Ping turning machine chain, B-shaped chocolate coating machine belt Net.
材质有A3低碳钢、45#钢、ICY13耐热钢、ICY18N19Ti钢、oocr18Ni14MO2Cu2耐酸耐热钢等。2、食品饼干行业:幅宽180mm-1200mm棉线,尼龙线帆布带,烤炉网带,冷却喷油机网带,平转弯机网链,巧克力涂层机乙形网带。
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- 推荐网络例句
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It is like a mustard seed, which a man took and planted in his garden.
好像一粒芥菜种,有人拿去种在园子里,长大成树,天上的飞鸟宿在他的枝上。
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The increase in narcissism was stronger for women than for men in both datasets.
在自我陶醉的增加是更有力的比这两个数据集男性。
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This human is in bardo with earth ' s counsels at this time.
这个人目前正处于中阴身中,和地球的法律顾问会呆在一起。