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In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2~0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%~80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0~0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法,将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合,建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺:胰酶用pH4.5醋酸缓冲溶液提取后,粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液:0.01mol/LNaoAc-HoAc缓冲液(pH4.5,洗脱缓冲液:0.01mol/LNaoAc-HoAc缓冲液(pH4.5)含0.2~0.35mol/LNaCl分两步洗脱,收集激肽释放酶的洗脱峰;DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液:0.01mol/LTris-HCl缓冲液(pH7.5,洗脱液:0.5mol/LNaCl溶液,一次洗脱,收集胰蛋白酶洗脱峰;最后,亲和层析未吸附液用30%~80%硫酸铵分级盐析处理,沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液:0.01mol/LNaAc-HAc缓冲液(pH4.5含1mol/L(NH_4)_2SO_4,洗脱缓冲液:0.01mol/LNaAc-HAc缓冲液(pH4.5)含0~0.6mol/L(NH_4)_2SO_4,分两步洗脱,收集糜蛋白酶的洗脱峰。

According to the simulated results, it was found that the incident light was not fully absorbed in the solar cell due to a thinner base layer. The un-absorbed incident light could be further reflected by a AuGe/Au mirror layer and absorbed again by the base layer. Thus the disadvantage of the GaAs thin-film cell with a thinner base layer could be solved by the additional reflection from the AuGe/Au mirror with a high reflectivity within 800~1000 nm wavelength. Moreover, the external quantum efficiency in the long wavelength region and short current density were also increased.

从模拟结果中得知,薄膜型结构之太阳电池可以经由AuGe/Au之金属反射层,再次将未被基极层完全吸收之入射光反射回太阳电池,使得薄膜型结构在长波段的外部量子效率获得提升,这结果显示薄膜型结构太阳电池可利用AuGe/Au之金属反射层在长波段的高反射率,改善使用较薄基极层时,无法将入射光完全吸收的缺点。

Photothermic and photochemical action will be triggered by the radiation of IPL. On one hand, IPL will promote collagen reproducing and recombining which makes the skin more flexible and smooth. On the other hand, the light with long wavelength can easily pass through epidermis layer and will be absorbed selectively in priority by pathological pigments which will be destroyed by high heat, and get the effect of remove pigmentations, vascular lesions and unwanted hair.

当强脉冲光照射皮肤时,会产生生物刺激作用和光热解作用,一方面使皮肤中的胶原纤维和弹力纤维再生并重新排列,恢复皮肤弹性,从而减轻皱纹;另一方面,强脉冲光能穿透皮肤,被组织中的病变色素团优先选择性吸收,热能在不破坏正常皮肤组织的前提下,击碎病变色素团,从而达到治疗毛细血管扩张,祛除色斑,和脱除多余毛发的效果。

It is nice to have a cup of cold drink after a meal.. However, the cold water will solidify the oily stuff that you have just consumed. It will slow down the digestion. Once this 'sludge' reacts with the acid, it will break down and be absorbed by the intestine faster than the solid food.

吃过饭之后喝一杯冰冷的饮料的确很舒服,然而,冷开水会使你刚刚吃下肚的油腻腻的食物凝结在一起,而且会使胃肠的消化作用变慢,一旦这一大坨像烂泥的东西碰到胃?

It! is nice to have a cup of cold drink after a meal.. However, the cold water will solidify the oily stuff that you have just consumed. It will slow down the digestion. Once this 'sludge' reacts with the acid, it will break down and be absorbed by the intestine faster than the solid food. It will line the intestine. Very soon, this will turn into fats and lead to cancer .

吃过饭之后喝一杯冰冷的饮料的确很舒服,然而,冷开水会使你刚刚吃下肚的油腻腻的食物凝结在一起,而且会使胃肠的消化作用变慢,一旦这一大坨像烂泥的东西碰到胃酸,就会分解而很快地被肠子吸引,它会附著在肠壁上,没多久它就会变成脂肪,而导致癌症,所!

It is nice to have a cup of cold drink after a meal.. However, the cold water will solidify the oily stuff that you have just consumed. It will slow down the digestion. Once this 'sludge' reacts with the acid, it will break down and be absorbed by the intestine faster than the solid food. It will line the intestine. Very soon, this will turn into fats and lead to cancer It is best to drink hot soup or warm water after a meal.!

吃过饭之后喝一杯冰冷的饮料的确很舒服,然而,冷开水会使你刚刚吃下肚的油腻腻的食物凝结在一起,而且会使胃肠的消化作用变慢,一旦这一大坨像烂泥的东西碰到胃酸,就会分解而很快地被肠子吸引,它会附著在肠壁上,没多久它就会变成脂肪,而导致癌症,所以说,吃过饭之后最好还是喝一碗热汤或一杯温开水。

It is nice to have a cup of cold drink after a meal.. However, the cold water will solidify the oily stuff that you have just consumed. It will slow down the digestion. Once this 'sludge' reacts with the acid, it will break down and be absorbed by the intestine faster than the solid food. It will line the intestine. Very soon, this will turn into fats and lead to cancer .. It is best to drink hot soup or warm water after a meal.!

吃过饭之后喝一杯冰冷的饮料的确很舒服,然而,冷开水会使你刚刚吃下肚的油腻腻的食物凝结在一起,而且会使胃肠的消化作用变慢,一旦这一大坨像烂泥的东西碰到胃酸,就会分解而很快地被肠子吸引,它会附着在肠壁上,没多久它就会变成脂肪,而导致癌症,所以说,吃过饭之后最好还是喝一碗热汤或一杯温开水。

It is nice to have a cup of cold drink after a meal.. However, the cold water will solidify the oily stuff that you have just consumed. It will slow down the digestion. Once this 'sludge' reacts with the acid, it will break down and be absorbed by the intestine faster than the solid food. It will line the intestine. Very soon, this will turn into fats and lead to cancer . It is best to drink hot soup or warm water aft! er! a meal.

吃过饭之后喝一杯冰冷的饮料的确很舒服,然而,冷开水会使你刚刚吃下肚的油腻腻的食物凝结在一起,而且会使胃肠的消化作用变慢,一旦这一大坨像烂泥的东西碰到胃酸,就会分解而很快地被肠子吸引,它会附著在肠壁上,没多久它就会变成脂肪,而导致癌症,所以说,吃过饭之后最好还是喝一碗热汤或一杯温开水。

It is nice to have a cup of cold drink after a meal.. However, the cold water will solidify the oily stuff that you have just consumed. It will slow down the digestion. Once this 'sludge' reacts with the acid, it will break down and be absorbed by the intestine faster than the solid food. It will line the intestine. Very soon, this will turn into fats and lead to can! ce! r. It is best to drink hot soup or warm water after a meal.

吃过饭之后喝一杯冰冷的饮料的确很舒服,然而,冷开水会使你刚刚吃下肚的油腻腻的食物凝结在一起,而且会使胃肠的消化作用变慢,一旦这一大坨像烂泥的东西碰到胃酸,就会分解而很快地被肠子吸引,它会附著在肠壁上,没多久它就会变成脂肪,而导致癌症,所以说,吃过饭之后最好还是喝一碗热汤或一杯温开水。

Efficacy: Unique formula can be absorbed by skin and provide nourishing and simultaneously control oil and skin and reduce spot possibility, making skin smooth and white.

功效: 独特的配方能被肌肤吸收,为肌肤提供滋润的同时亦控制油脂,调理肌肤,减低痘痘的出现,令肌肤光滑,白皙。

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