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Detection the early stage apoptosis cells by flow cytornetry Incubating SCL-1 cells in 6-well culture medium with 1×10~6/well, after 12～48hculture with the concentration of imiquimod 0μg/mL and 150μg/mL, gathering cellsgrowing along the wall and making monocellular suspension, washing in PBS for 2times, and then add 5μL Annexin V/FITC and 10μL PI solutions, reaction for 15min avoiding light, detection the early stage apoptosis cells by flow cytometry.

流式细胞仪测定细胞早期凋亡 SCL-1细胞以1×10~6个／孔接种于6孔培养板中，使用含0μg／mL、150μg／mL咪喹莫特的培养液培养12～48h，收集贴壁生长的细胞制成单细胞悬液，PBS洗两次，分别加入5μL Annexin V／FITC和10μL浓度20μg／mL的PI溶液，混匀后室温避光孵育15min，流式细胞仪检测，结果用Cell Quest软件分析。

These restrictive con d itions can be taken as practical design criteria for avoiding reactor instabilit y and temperature sensitivity in multitubular packed-bed reactors.

这些条件也可以作为避免列管式固定床反应器不稳定性和温度灵敏性的实用设计判据。

In addition to avoiding nephrotoxic agents in the setting of acute renal failure, medications that are eliminated by the renal route require dosing adjustments.

除了避免在急性肾功能衰竭，这是由肾脏清除药物的路线设置肾毒性药物需要剂量调整。

Medication Dosing in Renal Failure In addition to avoiding nephrotoxic agents in the setting of acute renal failure, medications that are eliminated by the renal route require dosing adjustments.

登录后回答可以获得经验值奖励，并可以查看和管理所有的回答。登录|在药物治疗肾功能衰竭的配料除了避免在急性肾功能衰竭，这是由肾脏清除药物的路线设置肾毒性药物需要剂量调整。

Avoiding cardiopulmonary bypass and nephrotoxic medications may be advisable for LL patients whenever feasible.

LL病患当可行时，应该被建议不要接受心肺绕道、或是使用具肾脏毒性的药物。

The December issue reports that stage 3 to 5 CKD is found in 4% to 5% of the adult population in the United Kingdom, most often in elderly individuals who have comorbidities for which they are prescribed several drugs. The authors remind practitioners that patients with impaired renal function have decreased drug clearance by the kidney and may be more susceptible to certain nephrotoxic drugs. They advise:"Whenever possible, should take steps to minimize these risks, by avoiding the drugs, using reduced doses or administrative frequency, or increasing the monitoring of renal function" by measuring estimated glomerular filtration rate.

12月号的报导刊载了英国人口中，有4%至5%是CKD第3至5级，大部分是老年人，且这些病患经常使用多种药物；作者提醒执业医疗人员，肾脏功能不全病患其肾脏排除药物的清除率下降，而且更可能受到某些具有肾毒性药物的伤害；他们建议，当情况允许时，执业人员应该将这些风险降到最低，透过避免使用部分药物，或是降低剂量、或频次，或是增加监测肾丝球廓清率的频率来评估肾脏功能。

By referencing to the nomograph, it is easy to find tension value at any load position within the span of suspended rope, avoiding complex calculation procedure with third degree tension equation.

使用该图表，可以方便地求出悬索跨内任一载荷位置的张力值，避免复杂的三次方张力方程的计算过程。

He invoked methodological considerations of the sort mentioned above (avoiding 'nomological danglers', he said).

他引用上面提到的（他说要&避免打着'法则学『nomological，法则、定律』'的幌子&）方法论上的考虑。

Aimed at 9-degree of freedom hyper-redundant module robot in intra-vehicular robot system, attention is given to the avoiding joint limits as an optimal mark. Two different iterative algorithms considering fault-tolerance for trajectory planning of redundant robot in Cartesian space are then proposed by using the method of differential locomotion. The first algorithm is for linear-path in which the pose of manipulator can be changed, the second is for circular-path in the plane decided by three noncollinear arbitrary points in the space. Experiments on the specimen robot platform are performed to validate the above algorithms.

以太空舱内机器人系统9-自由度超冗余模块机器人为研究对象，以避关节极限作为优化指标，考虑容错性能，利用微分运动的方法，分别提出了适于计算机迭代计算的冗余机器人在笛卡儿空间进行手爪姿态可变时的直线轨迹优化算法和手爪经过空间中任意不共线3点决定的平面内的圆弧轨迹优化算法。

Furthermore, we can discover that rational model have some nonnegligible positive functions, such as caring for policy outcomes, producing the systematic knowledge centering in problems, avoiding irrational trial and error method, promoting policy innovation to the hilt, being most likely to prevent individual judgment and experiential judgment from getting the run upon.

我们还可以发现，理性决策模式具有不可忽视的正功能，譬如关注政策结果、避免非理性试错、生产以问题为中心的系统知识、最大限度地支持和促进政策创新、最有可能防止个人判断和经验判断占上风。

Chimborazo and Cotopaxi, took me by the hand.

越过琴博腊索山和科托帕克西山。

This car is in a good condition.

这辆车的状况很好。