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Expression patterns of genes differentially expressed in starch metabolism and cell cycle during endosperm development7 DAP kernals, 15 DAP endosperms and 25 DAP endosperms of B73, ae/wx and sh1 were used to study the expression patterns of 18 differentially expressed genes in sugar metabolism and 9 differentially expressed genes in cell cycle. We found that the expression patterns of Pul, SBEⅡa, UGPase, SUS3, SSⅡa in sh1 and Sh2-1, SUS3, SSⅡa in ae/wx were altered, revealing that these genes may interact with each other.

碳水化合物代谢基因和细胞周期基因随胚乳发育的变化以B73、ae/wx、sh1授粉后7天种子、15天和25天胚乳为材料，分析18个糖代谢差异基因和9个细胞周期调节相关的差异基因的表达模式，研究发现sh1突变体中Pul、SBEⅡa、UGPase、SUS3和SSⅡa基因表达模式发生改变，ae/wx突变体中Sh2-1、SUS3和SSⅡa基因表达模式发生改变，揭示了突变体对这些基因的巨大影响，反映了它们可能存在相互作用。

In a word, we extracted first cotyledon of buckwheat total RNA, by method of RT-PCR and 3RACE, get the C terminal cDNA sequence of PI from Ukraine buckwheat, and do some bioinformatics analyse on this cDNA sequence of PI from buckwheat, such as gene characteristics, expression mode. All of this will be help for the forward research on functional protein and high performance of procaryon expression or eukaryon expression.

综上所述，我们利用RT-PCR和3′RACE技术首次从吉乌一号甜荞第一对子叶中克隆获得荞麦蛋白酶抑制剂基因并对其基因特性、表达模式进行生物信息学分析，同时构建了该基因序列羧基端原核表达载体，为今后进一步研究荞麦功能性蛋白的分子机制及高效原核、真核表达载体的构建奠定了基础。

Cell lines were co-tranfected with bi-plasmids and cultured at 1% O〓 condition, luciferase assay demonstrated that all of the expression systems with different domains could be hypoxia-activated. GAL-CAD achieved more than 100-fold induction in A549 cell and more than 40-fold in HepG2; expression level of the system markedly increased after addition of glycin arm, almost 10-fold enhancement in HepG2 cell. Because basic expression also increased, the induction fold by hypoxia decreased.

双质粒共转染不同细胞株后，在1％O〓环境下培养24h，荧光素酶活性检测结果提示含不同结构域的表达系统均有受缺氧激活的能力，其中GAL-CAD在A549中获得100倍以上的诱导，在HepG2中获得40倍以上的诱导；加入甘氨酸臂后该表达系统的转录活性大为增强，在HepG2细胞中增强了10倍以上，但是由于基础表达也有所增加，受缺氧诱导的倍数下降。

Expression analysis with RT-PCR revealed that PcLGBP gene was exclusively expressed in hepatopancreas and the expression could be up-regulated by heat-killed Listonella anguillarum. The binding specificity of PcLGBP to ligand were subsequently examined by recombinant expression of the cDNA fragment encoding its mature peptide in Escherichia coli BL21 (DE3) and ELISA approach.

为分析PcLGBP基因的功能，通过半定量RT-PCR方法研究了其在组织中的分布，并用荧光定量PCR技术分析了其对细菌刺激的响应，结果表明PcLGBP基因是肝胰腺特异表达的基因，能响应热致死鳗弧菌刺激而增强表达，说明PcLGBP基因可能参与螯虾的细菌防御反应。

Inhibition of hepatic NF?κBp65 expression,and Kupffer cell p38MAPK protein expression and phosphorylation p38MAPK protein expression may be one of their therapeutic mechanisms of the above therapies for fatty liver.

抑制NF？κBp65和p38MAPK蛋白及磷酸化p38MAPK蛋白表达可能是上述不同治法方药抗脂肪肝作用的机制之一。

From the medle ages sculpture,we can see that people in that period take human sculptures merely as a decorative part of an achetecture;The Rassanance make people regained their natural inner strength,and people express their admiration to the heros,using the pose of human sculpture. The great master Rodin reach the peak in making the full use of human pose,as a way of artistic expression. While doing so. he turned the the development of sculpture into a new track-the way of expression itself. From then on,in the hands of some mordern sculptors,human pose and the distinctive personal expression ways of each sculptor and the matiral.

运动。均衡等理想美的追求；中世纪教堂人物雕塑姿势的僵直和微妙反映了当时雕塑成为建筑的依附以及教徒对上帝的态度；文艺复兴使人们找回自身的力量，对英雄的崇敬和力量的赞美通过人物雕塑充满生机的姿势体现出来；罗丹对人物姿势表现力的把握达到了一个高峰，同时他把雕塑引向了形式本身，在他以后的一些现代雕塑家手中，人物姿势和艺术家各具个性的艺术手法。

At the same time, the process of the wound healing was observed histologically, and the regeneration cycle of epidermal cells and the temporal change in inflammatory cells were measured. Results Inflammatory cells infiltrated into wound surface were neutrophils, followed by macrophages and lastly lymphocytes. Epidermal cells proliferated most actively on PBD 3 and the mitoses of them increased significantly on day 7 after burn. The gene expression of PDGF, PDGFR and EGFR reached peaks on PBD 1 and the gene expression of EGF and TGFβ-R2 were highest on PBD 3. In addition, the gene expression of TGFβ-R1 and TGFβ1 increased significantly on PBDs 5 and 7 respectively.

结果 炎性细胞到达创面依次为中性粒细胞、巨噬细胞和淋巴细胞；表皮细胞周期变化为伤后3天细胞增殖活跃，伤后7天细胞分裂明显增多；伤后1，3天PDGF、PDGFR(血小板源性生长因子受体)和EGFR基因表达明显增强，伤后3，5天EGF、TGFβ-R2基因表达增强，伤后5，7天TGFβ-R1(转化生长因子β受体1)基因表达增强，伤后7，10天TGFβ1基因表达增强。

The attention of Yin Chaohui in 2008 is not only gradually extended from the expression of "hands" to that of "skin", in his pictures he intentionally added expressions of the texture of "skin", but also, corresponding to the texture of "skin", added details of "spots","wounds","scabs","naevi" and so on in a way of adding inflammatory details, which undoubtedly compose the detailed description of Yin Chaohui to "expressions" of "skin", moreover, Yin Chaohui continuously combined the expression of "hands" with "face" and "body" and hence explored more abundant and changeable picture vocabulary and more capacious space of expression.

2008年尹朝辉创作的关注点，不仅从对&手&的表情逐渐延伸到对&皮肤&的表情，他在画面中有意识地增添了对&皮肤&的质感的表达，并对应于&皮肤&的质感而&添油加醋&般地去添加了那些皮肤上的&斑点&、&伤口&、&结痂&、&痦子&等细节，这些无疑都构成了尹朝辉对于&皮肤&的&表情&的细致化描述，而且尹朝辉还不断将&手&的表情和&脸&、&肢体&结合起来，拓展出了更为丰富变化的画面语汇和更大的表达空间。

Results: MMP2 gene expression could be detected in norma l subject. MMP2 gene expression levels in patients with chronic hepa titis and liver cirrhosis were higher than that of normal control. Th e gene expression level in patients with chronic hepatitis was higher in comparison with that in patients with liver cirrhosis.

结果：正常人有明胶酶A(MMP2)的基因表达；慢性肝炎、肝硬化患者MMP2基因表达较正常对照明显增强；慢性肝炎患者MMP2基因表达水平明显高于肝硬化患者。

I draw people through the test, understand the different classes of children aged people in figure painting tests on the following characteristics: 1, small classes can not find child care because of the graphic match, the majority of child care both in the overall figures are still details to chaos The lines to express disorder. 2, Zhongban due to the continuous improvement of cognitive, has been able to find a match in the minds of the graphics, and has been in the minds of the graphic expression, but the details painted on the expression of often rough. 3, taipans child care whether or details on the whole, are more perfect expression, but the large class paintings by children of mothers are the embodiment of their own, can not distinguish between adults and children.

笔者通过画人测验，了解不同年龄班的幼儿在人物画人测验上有以下的特点：1、小班幼儿由于找不到匹配的图形，大部分幼儿无论是在人物整体上还是细节上都是用混乱无序的线条来表达。2、中班由于认知的不断提高，已经能在头脑中找到相匹配的图形，并将头脑中已有的图形表达出来，但是在细节描绘上表达的往往比较粗略。3、大班幼儿无论是在整体上还是细节上，表达的都比较完善，但是大班幼儿所画的妈妈都是自己的化身，不能区分大人和小孩。

Many will continue to choose to live in duality and in conflict.

许多人将继续选择活在二元对立性和冲突中。

I find that students of the University of Physical Education all wear sportswear at first sight.

我发现：体育大学的学生乍一看，都是穿运动衣，大家都一样