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OLH or 8-bromo-cAMP were added to luteinized granulosa cells. Results indicated that, protein expression of HIF-1a, HIF-1b and VEGF had a better performance in hypoxia than in normoxic condition, but phospho-PKA, StAR and P450scc protein expression had weaker expression in the former condition than in the later one. As a result of decreasing secretion of progesterone the decreasing of phospho-PKA was suspected to have a role in the reduction of StAR and P450scc in the condition of hypoxia.

结果发现，黄体化粒性细胞於缺氧条件下其HIF-1a、HIF-1b与VEGF蛋白质表现量皆较正常氧高；同时，StAR及P450scc蛋白质表现量则降低，但3b-HSD不受缺氧影响；此外，藉由特异性抗体检测PKA讯息传递路径发现，PKA讯息传递路经於缺氧条件下磷酸化PKA（phospho-protein kinase A）蛋白质表现量下降，可能经此路径的调控造成StAR及P450scc蛋白质表现量下降，导致黄体化粒性细胞孕酮分泌量减少。

When expression the knowledge we integrate object oriented expression and produce formula expression, and flexible deal with knowledge with different profundities.

在知识的表示中，集面向对象的表示和产生式表示为一体，并对不同深度的知识予以灵活处理。

The thymuses, spleens, sera and anticoagulant blood were collected after the rats were killed, to carry out these works:(1) Zinc concentrations were analyzed by atomic absorption spectrophotometry;(2) Body weight and the weight of thymuses and spleens were measured, the organ index were calculated;(3) Changes in pathology of thymus and spleen were observed;(4) Using TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) method, the apoptosis of thmocytes and spleen lymphocytes was checked;(5) The expression of bcl-2、bax mRNA in thymus and spleen were detected by RT-PCR (reverse transcription polymerase chain reaction);(6) The expression of p56〓, a signal transduction protein in thymus and spleen were detected by immunohistochemistry;(7) Two-color cytofluorometric analysis was used to assess the expression of CD4, CD8, CD45RA and CD45RC in peripheral blood lymphocytes.

动物处死后留组织、抗凝血及血清，进行以下检测：(1)原子吸收法测血清锌浓度；(2)测胸腺、脾脏脏器重量并与大鼠体重比较，计算脏器指数；(3)HE染色对胸腺、脾脏进行病理观察；(4)原位末端标记法测胸腺、脾脏中淋巴细胞的自发凋亡，并通过图像分析进行比较；(5)逆转录聚合酶链式反应检测凋亡调控基因bcl-2、bax mRNA在免疫器官中的表达；(6)免疫组化方法检测信号转导蛋白p56〓在免疫器官中的表达；(7)流式细胞术分析外周血淋巴细胞中CD4+、CD8+、CD45RA+及CD45RC+CD4+、CD45RC-CD4+细胞的数量和比例。

But there are no correlations between gene expression and gene expression specificity or gene expression level.

有趣的是，基因的组织特异性和基因的表达量与基因表达的进化则可能没有相关性。

Results VEGF-C and VEGFR-3 are all expressed on both mesenchymal cells and vascular endothelium of the embryos during early period. In middle and latter period, VEGFR-3mRNA is mainly expressed on cell plasm of alimentary canal, but it has no expression on vascular and lymphatic endothelium; The expression of VEGFR-3 is extensive and positive expression is seen on the vascular and lymphatic endothelium.

在胚胎中、后期消化道组织中VEGF-C主要表达于消化管壁组织细胞以及肠系膜内，在血管和淋巴管内皮细胞则无表达；VEGFR-3亦表现为广泛的表达，于血管和淋巴管内皮细胞可见阳性表达，并随着胚胎的发育，其在淋巴管内皮细胞的阳性表达率明显增加。

In the functional mechanism, we should use the technique "use the DNA Microarray to build the gene expression difference chart" to lead to the combination of Traditional Chinese Pharmacy and gene expression difference chart to study. Meanwhile,we can establish a totally new method of screening modern Traditional Chinese Pharmacy based on the gene expression difference chart, it can really make the modernization and internationalization of Traditional Chinese Pharmacy.

在中药作用机制方面，应采用&利用基因芯片建立基因表达差异谱&技术，将中药与基因表达差异谱有机地结合起来进行研究，同时以基因表达差异谱为基础建立一种全新的现代中药筛选方法，使中药真正实现现代化、国际化。

The results of SS investigating indicated that the SS protein expression in leaf at seedling stage was high comparatively, when at jointing and heading stage, SS expression was low in leaves and stems, along with sorghum development, since flowering stage, SS expression increased sharply, decreased at dough stage.

2对SS表达检测结果表明，甜高粱苗期叶片中SS蛋白质表达量较高，拔节、抽穗期叶片和茎中SS蛋白质表达量较低，随着高粱的生长发育，开花以后叶片和茎中SS的表达量普遍升高，腊熟期略有下降。

To better understand the mechanism of sucrose accumulation, the correlation of sucrose content and sucrose phosphate synthetase、sucrose synthase expression was analyzed by monitoring the expression of SPS in leaves and stems.(1) The results of SPS investigating indicated that the sucrose content, the expression of SPS in leaves and stems were increased along with the growth of sorghum, reached the highest level at grain filling stage, and decreased at dough stage.

1对SPS表达检测结果表明，在高粱的生长发育过程中，茎中蔗糖含量、叶片和茎中SPS蛋白质表达量持续上升，灌浆期达到最高，腊熟期略有下降，它们之间的变化趋势基本一致，甜高粱茎蔗糖含量与叶片SPS蛋白质的表达相关系数为0.895，与茎SPS蛋白质的表达相关系数为0.781；甜高粱和普通高粱相比，其叶片中SPS表达量差别不明显，而甜高粱茎中SPS表达量明显高于普通高粱。

Results The activation of NF-κB, proliferation response, and expression of IL-4 and I L-5 mRNA and protein in T lymphocytes stimulated by PMA were significantly hig her than those of their blank control (P.01), while those indexes of T l ymphocytes stimulated by PMA and PDTC simultaneously were significantly lower th an those stimulated by PMA alone (P.01). The apoptotic index of T lympho cytes stimulated with PMA were significantly lower than that of their blank cont rol (P.01), and the apoptotic index of asthmatic guinea pig T lymphocytes stimulated with PMA and PDTC simultaneously were significantly higher than that stimulated by PMA alone (P.01). The significant positive correlations w ere found between the activation of NF-κB and the proliferation (r=0.64, P.001), and the expression of IL-4 and IL-5 mRNA and protein of T lymph ocytes, respectively (r=0.55-0.68, P.001). There was also signific ant negative correlation between the activation of NF-κB and apoptosis of T l ymphocytes (r=0.62, P.001). Conclusions NF-κB may participate in the signal conduction of PKC regulated proliferation, apoptosis and expression of IL-4 and IL-5 of T lymphocytes in asthma.

结果加入PMA培养的哮喘组T淋巴细胞NF-κB的活化、细胞增殖反应、IL-4和IL-5的mRNA和蛋白质的表达均显著高于其空白对照(P.01)，而同时加入PMA和PDTC培养的哮喘组T 淋巴细胞以上指标均显著低于只加入PMA培养的哮喘组T淋巴细胞(P.01)；加入PMA 培养的哮喘组T淋巴细胞的凋亡指数显著低于其空白对照(P.01)，而同时加入PMA 和PDTC培养的哮喘组T淋巴细胞凋亡指数显著高于只加入PMA培养的哮喘组T淋巴细胞(P 。01)。T淋巴细胞NF-κB的活化与增殖反应呈显著正相关(r=0.64，P.00 1)，与IL-4和IL-5的mRNA和蛋白质的表达也均呈显著正相关(r=0.55-0.68，P 。001)，而与凋亡指数呈显著负相关(r=-0.62，P.001)。

OBJECTIVE To evaluate the expression of epidermal growth factor receptor as a molecular target for oropharyngeal squamous cell carcinomas measured by automated quantitative analysis and its significance of EGFR expression, especially nuclear EGER expression in the prognosis of cancer.

目的 评价自动定量分析法(automated quantitative analysis， AQUA)测定表皮生长因子受体(epidermal growth factor receptor EGFR)在口咽部鳞状细胞癌组织中的表达水平，了解其在判定预后中的价值。

Pachomius manual labour was organized as an essential part of the monastic life; and since it is a principle of the monks as distinguished from the mendicants, that the body shall be self-supporting, external work of one sort or another has been an inevitable part of the life ever since.

根据圣pachomius体力劳动组织作为一个重要组成部分寺院生活；以来，这是一个原则的和尚，作为有别于乞丐，该机构应自支撑，对外工作的这种或那种已一个必然的一部分，生活至今。

In order to study the effects of parametrical rolling on the ship movement and tilts, in allusion to ship's navigating in regular following waves, the equation of ship rolling motion is transformed into normal Mathieu equation by proper variable replacement when strong parametrical excitation in restoring moment varied according to sine or cosine wave.

为研究参数横摇对船舶运动和倾覆的影响，通过对船在规则波上随浪航行时，恢复力矩中出现的1个以正余弦规律变化的强参数激励项进行适当的变量置换，将船舶的横摇运动方程转换成标准形式的Mathieu方程。