查询词典 arithmetic expression

Basing on the Graf′s addition theorem, two expessions, interior and exterior expression, are deduced and the suitable problems for them are discussed. It has been found that the interior expression is the main point for sovling the scattering problem of circular inclusion in half-space. It also has been found that the exterior expression is the key for the scattering problem of circular arc canyon and circular arc alluvial valley, and is one of the main points for scattering problem of circular arc hill as well.

从Graf原有加法公式出发，本文给出了其内域型和外域型两种表达形式并讨论了这两种表达的适用范围，同时指出：内域型表达是求解半空间内含夹塞区出平面散射问题的要点，而外域型表达是解决任意圆弧型凹陷地形和任意圆弧型沉积盆地出平面散射问题中求解难点的关键，同时也是克服任意圆弧型凸起地形出平面散射问题求解困难的要点之一。

In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型，单独应用外引物进行一次扩增，可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；而在此基础上再次应用内引物扩增，则可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；在用RNA模拟的肿瘤细胞转移模型中，两次扩增可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中，2例没有检测到EWS/FLI1 mRNA的表达，分别随访8个月和30个月，没有发现临床转移的证据；对其余9例阳性表达的病例进行半定量的研究，6例EWS/FLI1 mRNA高表达的病例，分别在术后2-24个月发生转移，而3例EWS/FLI1 mRNA低表达的病例，则至今没有发现转移的证据。

Along with the fluconazole solution density rise,the experimental two kind of strain various glucose density is higher,showe d the glucose consumption are less,takes the logarithmof the medicine de nsity,discovered the logarithm the medicine density and each glucose den sity presents the linear relations;Carries on the analysis comparison to under the fluconazole function two kind of strain linear relations,disc overed the relations of the two strains has the nonuniformity.3 Compare the fluconazole induction reaiatance SC5314 strain and sens itive strain compares,its difference gene expression mainly concentrates in:The code proteinase body and the protein hydroltyic enzyme gene,in the code sugar fat metabolism process is connected the protein gene,the cell cycle correlation gene,the duplication and the translation adjustme nt correlation gene,the stress response correlation gene,the line plast ochondria correlation gene,the cell wall function related gene.4 Candida albicans SC5314 induction resiatance strain was processed b y Xianglian solution,its expression change gene mainly is:Code stress re sponse family protein gene,biomembrane relevant gene,a code proteinase body gene race,code cell cycle related protein gene,duplication and tra nslation adjustment related protein gene.5 The clinical reaiatance strain Candida albicans was processed by Xi anglian solution,its expression change gene mainly is:Codes the hot sho ck protein gene,the serine/threonine protein activating enzyme gene,the proteinase body family gene,the regulation copies and translates the ge ne.

随着氟康唑药液的浓度上升，试验的两种菌株各孔葡萄糖浓度越高，说明葡萄糖消耗越少，经过药物浓度取对数后进行分析，发现取对数后的药物浓度和每孔中葡萄糖浓度者呈现线性关系；对氟康唑作用下的两种菌株的线性关系进行分析比较，发现对两种菌株作用具有不一致性。3氟康唑诱导的耐药SC5314菌株与诱导前的敏感株相比，其差异基因表达主要集中在：编码蛋白酶体及蛋白水解酶的基因，编码糖脂代谢过程中相关蛋白的基因，细胞周期相关基因，转录及翻译调节相关基因，应激反应相关基因，线粒体相关基因，细胞壁功能相关基因。4白念珠菌SC5314诱导耐药株经香莲外洗液作用后，其表达变化的基因主要是：编码应激反应家族蛋白的基因，生物膜相关性基因，编码蛋白酶体基因一族，编码细胞周期相关蛋白基因，转录及翻译调节的相关蛋白基因。5白念珠菌临床耐药菌株经香莲外洗液作用后，其表达变化的基因主要是：编码热休克蛋白基因，丝氨酸/苏氨酸蛋白激酶基因，蛋白酶体家族基因，调控转录及翻译基因。

Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础，构建了原核表达载体PQE30a／FeSOD，经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后，再转化入大肠杆菌M15中，通过IPTG诱导，得到高效体外表达，经SDS-聚丙烯酰胺凝胶电泳检测，表达的目的蛋白占总菌体蛋白的37％，可溶性和包涵体两种形式均有存在，上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析，表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

The results are as follows: 37℃ is the most favorable temperature for induction expression of SUMO-hEGF in BL21, 5.0g/L the most best concentration of arabinose, and 4h the best time for induction expression. Meanwhile, the quantity of expression is around 20.2%. The conclusion that hEGF is the purified protein is verified by Western blotting. It is a good foundation for the development of hEGF genetic medicine.

结果表明： SUMO-hEGF在BL21中的最佳诱导表达温度为37℃、诱导剂阿拉伯糖的最佳浓度为5.0g/L、最佳诱导表达时间为4h，表达量约为20.2%；Western blot 分析证实，纯化后的蛋白是hEGF，为进一步开发hEGF基因工程药物奠定了基础。

Decreased expression of RIG-I in human hepatocellular carcinomaFirst, with tissue array, we analyzed the expression of RIG-I in HCC, and found that 90%(27/30) of para-tumor samples were positive for RIG-I expression, but only 20%(6/30) of HCC samples were RIG-I positive.

然而，在30例肝癌患者的肝癌组织中，只有5例患者的癌组织中RIG-I蛋白表达为阳性，1例患者的癌组织中RIG-I蛋白表达为弱阳性，阳性率为20%。

The complex alterations of gene expression underlie the development of malignant phenotype of lung squamous cell carcinoma and the Atlas cDNA expression array is a useful method for describing the expression profiles.

Atlas微阵列表达分析滤膜是研究基因表达谱的较好方法。

The differential expression profiles of 588 known genes were determined using Atlas human cDNA expression array. Fifteen genes were differentially expressed following the overexpression of BNIPL-1. Most of them were involved in cell proliferation or cell apoptosis. Furthermore, the differential expression result was confirmed by semiquantitative RT-PCR.

然后采用人Atlas cDNA expression array对新基因BNIPL-1在细胞内的下游调控事件进行了初步研究。588个已知基因中有15个的表达发生了改变，其中大部分是细胞生长和凋亡相关基因，半定量RT-PCR验证了杂交结果的可靠性。

To better understand the roles of LsrA protein in nodulation, a series of analyses of nodules using scanning electron microscopy, genetic and biochemical approaches have been carried out. Our analyses suggest that the lsrA1 nodules contain bacteroids in the invasion and establishing zone only. The lsrA gene expression is active early in the invasion zone and activated later than bacA genes. The lost of LsrA protein dramatically reduced the expression of nifA and fixK, and completely blocked the expression of the nifH gene for nitrogenase. LsrA protein functions early in the bacteroid development and it is essential for the development nitrogen fixing bacteroids.

前期的工作中，苜蓿中华根瘤菌Rm1021中90个候选LysR基因已经被定向插入突变，并筛选在自生生长时期、共生生长时期的表型，以期寻找更多在自生状态或共生固氮中有功能的LysR转录因子。1 针对前期鉴定出的共生固氮必需的lsrA基因，我们应用了一系列扫描电子显微镜技术、生物化学、分子遗传学等方法，发现lsrA基因主要在根瘤侵染区开始表达，表达时序也在侵染阶段左右，但晚于bacA基因表达；LsrA蛋白缺失后根瘤固氮区中缺乏具有固氮能力的类菌体，nifA和fixK基因的转录水平降低，nifH基因的转录被完全阻断，因此LsrA蛋白为根瘤发育所必需，是新的根瘤发育信号传导途径成员。2 通过表型筛选我们鉴定了苜蓿中华根瘤菌的oxyR基因，并研究了它的调节特性。oxyR突变后，苜蓿中华根瘤菌对过氧化氢敏感性提高，适应性降低。

The result showed that there was no expression in the normal control group and an apparent expression of ERK1/2 and Elk in the asthma group. A rather dense expressing of ERK1/2 was at bronchiole and mucous membrane, sub mucous membrane, smooth muscle, cytoplasm and nuclei of the out layer of the smooth muscle cell and an expression of positive fiber at submucous membrane.

结果发现正常肺内没有发现ERK1/2和Elk的表达，而哮喘时肺内有明显的ERK1/2和Elk表达，ERK1/2较密集表达在小支气管和细支气管的粘膜层、粘膜下层、平滑肌层和平滑肌外层细胞的胞浆和胞核中，也可见粘膜下层有阳性纤维表达。

When this condition occurs, inbound replication with the source partner is stopped on the destination domain controller and event ID 2042 is logged in the Directory Services event log.

计算机密码学是研究计算机信息加密、解密及其变换的科学，是数学和计算机的交义学科，也是一门新兴的学科。

Instructions: click on the thumbnails to see a larger image, then use the left-right arrow keys to scroll through the slideshow.

使用说明：滑鼠点在小图上即可放大观赏。开启后键盘左右键可用来换照片。