查询词典 arbitrary sequence

A pair of specific primers was designed according to the reported cDNA sequence of the DC-ACO(Dianthus caryophyllus L.ACC Oxidase),and a partial sequence of the gene was amplified from genomic DNA of a carnation variety 'Master' by PCR.

据已报道的香石竹氨基环丙烷羧酸氧化酶基因的cDNA序列设计特异引物，以香石竹品种&Master&基因组DNA为模板，用PCR方法克隆出香石竹ACO基因的部分序列。

The C18 known sequence, whose sequence had been known but function remained unknown, was expressed in cataractous lens epithelium but was not expressed in normal lens epithelium.

序列已知而功能未知的C18已知序列在白内障晶状体上皮有表达，但其在正常晶状体上皮中无表达。

The nucleotide sequence of Cervus nippon FSH-β subunit was compared with other animals. It had the highest homology among Cervus nippon, sheep, goat and bovine in nucleotide sequence, which reached 92％~93％. There were 24~30 nucleotides difference among them.

将梅花鹿FSH-β亚基基因核苷酸序列与其它动物相比，结果显示梅花鹿与绵羊、山羊、牛的同源性最高，达到92％~93％，它们之间有24~30个核苷酸不同。

In this study, 526 base pair DNA fragment of OMT were obtained from Chamaecyparis formosensis. Then the 5' and 3' RACE coupled with the Genome walking were used to clone the full length of CCoAOMT, named CfCCoAOMT. After combining the sequence data, there is a total of 1721 bps in full length including promoter sequence.

本研究於红桧试材中，选殖出526个碱基对之Caffeoyl CoA 3-O-methyltransferase片段基因序列，再经由5'和3' RACE及Genome walking，共获得计有1721个碱基对，包含启动子及基因全长，命名为CfCCoAOMT。

The main findings of the study are as follows:(1) Both N2 and P3 are the characteristic ERPs under nogo inhibition,but P3 amplitude is a good indicator of inhibition processing depth;(2) Through ERP waveforms separation from encoding of stimuli sequence,we verify that the capacity of visual working memory is about 4 units;(3) In the go/nogo sequence response, encoding is mainly the function of right hemisphere, but inhibition retrieval is concentrated on left frontal area and anterior cingulated cortex.

本研究获得了以下结论：(1)在nogo抑制条件下，N2和P3波是特征的抑制波，但P3的波幅是反应抑制加工深度的良好指标；(2)对刺激的顺序编码加工的ERP验证了工作记忆的处理单元的容量为4左右；(3)在Go/nogo序列反应中，编码加工体现为右脑优势，而抑制提取的加工负波集中在左脑前额和前角回区。

The genetic DNA was extracted from the blood of 6 A. forsteri which sex was unknown. The PCR amplified fragments with primers P2/P8 were cloned in T-Vector. Taking homologous sequence of Circaetus gallicus as reference, the CHD gene sequence was compared to the homologues in GenBank to identify its sex.

方法］采用苯酚：氯仿抽提法提取6只未知性别的帝企鹅血液中的基因组DNA，运用P2/P8引物扩增CHD基因片段，将PCR产物克隆到T-Vector，利用NCBI的Blast程序，以短趾鹰同源序列为比对参照，将帝企鹅CHD基因片段序列与GenBank中的基因片段序列进行同源性比较分析，鉴定帝企鹅的性别。

Materials and Methods: Comparetive study between 2D - SE sequence and 3D-CISS sequence was made for lesions detection and diagnostic accuracy in 8 cases of intraventricle and 12 cases of cisternal diseases.

材料和方法：对照分析8例脑室和12例脑池占位病变的常规SE序列和3D—CISS的检出率和诊断正确率。

An otherwise healthy 12-month-old girl presented for evaluation of reduced abduction of the left eye detected at 6 months of age. The remainder of the examination was unremarkable. A special MRI sequence-fast imaging employing steady-state acquisition-visualized the right but not the left sixth nerve cisternal segment. This is the first reported use of the MRI FIESTA sequence to diagnose aplasia of the sixth cranial nerve.

应用快速平衡稳态取像核磁技术检测第六颅神经发育不全：本研究首次报道利用 MRI FIESTA 诊断第六颅神经发育不全，一例12个月的婴儿其他各项指标正常，在6个月时发现左眼诱导注视减少，其他的检查未见明显异常，利用 MRI FIESTA 可检测到右侧第六颅神经，但左侧部分检测不到。

A first embodiment comprises a first common primer sequence, a first template homology region, a cleavable site, a second common primer sequence and a second template homology region.

第一个实施方案包含第一通用引物序列、第一模板同源性区、可切割位点、第二通用引物序列和第二模板同源性区。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Many will continue to choose to live in duality and in conflict.

许多人将继续选择活在二元对立性和冲突中。

I find that students of the University of Physical Education all wear sportswear at first sight.

我发现：体育大学的学生乍一看，都是穿运动衣，大家都一样