查询词典 antibody

After born, blood groups, Coomb's test, free antibody and antibody release test were done for those 286 babies who were in high risk of HDN, so that we could understand the relationship between the antibody IgG level of pregnant women and ABO-HDN....

结果：2741例O型孕妇中，IgG抗-A效价4、64、128、256和≥512各组分别占61.9%、17.0%、12.6%、6.1%和2.4%。286例母婴血型不合且有ABO-HDN危险的孕妇所生新生儿中共有71例HDN阳性，占24.8%；孕妇IgG抗-A效价为64、128、256和≥512各组HDN阳性率分别为10.7%、28.3%、44.9%和64.3%(P.01)。

Objective Establishment of a hybridoma cell line secreting a monoclonal antibody to facilitate iˉdentification of human tissue kallikrein（KLK1gene,hK1protein）.Methods Mice were immunized with E.coli-exˉpressed GST-hK1fusion protein and their spleen cells were fused with SP2/0myeloblastoma cells.Specificity of the monoclonal antibody was shown by Western blotting and by immunofluorescence.Results The monoclonal antibody reacted specifically to E.coli-expressed hK1and with the KLK1cDNA-transfected COS-1cells.

目的 将人组织激肽释放酶（基因命名 KLK1，酶命名为hK1）cDNA克隆入肠杆菌表达质粒，以重组菌表达的GST-hK1融合蛋白免疫小鼠，获得了分泌hK1特异单克隆抗体的杂交瘤细胞株方法将KLK1cDNA克隆入真核表达质粒，用获得的重组质粒转染COS-1细胞，经间接免疫荧光试验证明，上述单克隆抗体能与转染细胞发生特异反应。

Keratin antibody IgM 3B4. CONCLUSION: Natural monoclonal anti??keratin antibody IgM 3B4 can inhibit the adhesion of Candida albicans to the malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein, and decrease the invasion of Candida albicans due to its inhibitory action on the formation of Candida albicans germ tube,which indicates that natural antibody IgM plays a role in the natural immunity against yeast infection.

单克隆天然抗角蛋白抗体IgM 3B4在体外能够抑制白念珠菌芽管形成，能够抑制白念珠菌与人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞的粘附，从而降低白念珠菌的侵袭力，提示天然抗体IgM在机体抗真菌感染的天然免疫中可能具有积极的作用。

The antibody is bound to an inert polymer support ,then exposed to reacts with the antigen at a different epitope is added. The second antibody used is one that has an enzyme attached to it that coverts a colorless or nonfluorescent substrate into a colored or fluorescent product. The amount of second antibody bound, and hence the amount of protein antigen present in the original sample, is determined by quantification of the intensity of color or fluorescence produced.

将抗体结合在一惰性聚合物载体上，然后与样品接触，洗脱未经结合的蛋白质加入与抗原其他表位也可以结合的另一种抗体，在所用的第二抗体上结合有酶，此酶可把无色的或无荧光的底物转化有色或显示荧光的产物。

The involvement of oxidization of peroxid in the antigen-antibody reaction makes the cell membrane permeability enhanced, so the fluorescence-labeled antibody stain can infiltrate into the cell easily and contact the antigen rapidly and diffusely, and then the antigen-antibody crosslinking can be formed efficiently.

利用过氧化氢的氧化作用参与抗原抗体反应，使细胞的膜通透性增强，利于荧光标记抗体染液渗入，从而更快速、广泛地接触抗原，更有效的抗原抗体的嵌合交联。

Aim To develop a sandwich ELISA for measuring serum β2-glycoprotein I-lipoprotein complex (β2-GPI-Lp concentration, and to explore the existence of serum complexes β2-GPI-Lp and its clinical value for IgA nephropathy. Methods ELISA was established using rabbit anti-human β2-GPI antibody as the capture antibody, and quantitating with polyclonal antibody against apolipoprotein enzyme conjugate.

目的 建立检测人血清β2-糖蛋白I-脂蛋白复合物的酶联免疫吸附法，探讨该复合物存在于人血循环中的可能性，并通过对IgA肾病患者的检测分析该复合物的临床诊断价值。

The purpose of this study was to explore the clinical value of the platelet antibody screening and typing in platelets transfusion by using microcolumn gel immunoassay. The platelets antigen??antibody reactions including the antibody screen and blood crossmatch were detected by MGIA.

为了探讨微柱凝胶免疫分析技术在血小板输注中血小板抗体筛选和配型的临床应用价值，运用微柱凝胶免疫分析技术检测血小板抗原抗体反应，包括抗体筛检、交叉配血。

Methods Phosphotyrosine was first combined with hemocyanin from keyhole limpets. Then, rabbits were immuned with the complete antigen and antisera were gained. The ELISA system consisted of purified antiphosphotyrosine antibody as first antibody and sheep antirabbit lgG-HRP as second antibody. Results CV within the batch was 8.4%.

应用合成的磷酸酪氨酸-钥孔帽贝血蓝蛋白免疫家兔得到抗血清，将纯化的抗磷酸酪氨酸抗体作为第一抗体，羊抗兔Ig酶结合物作为第二抗体，建立双抗体夹心酶联免疫吸附测定法。

In a third reaction the amount of bound detecting antibody in the coating antibody-proinsulin-detection antibody complex is visualised by use of streptavidin-peroxidase that binds to the biotin label.

该方法的灵敏度为0.6306miu/L，与142pmol/L的胰岛素原、3960pmol/L的C肽无交叉反应，平均回收率为97.12％，批内与批间变异系数分别为11.65％与5.95％，线性标准范围为0.63～100miu/L。

Pneumophila serogroup 5 antibody to develop two immuo-colloidal gold tests respectively. Main works listed below as:1、Preparation and identification of polysaccharide antigen of L. pneumophila Bacteria of LP1 ~ 7,9 and 10 were cultured on yeast agar buffer activated carbon for three to five days at 37℃, 5%CO2. Protein-free polysaccharide antigens were obtained after harvest in cells, extraction, deproteinization, dialysis and other steps. Their immunogenicities were verified by ultraviolet spectrophotometer full-wavelength scanning and Western blotting.2、Preparation and identification of rabbit anti-LP1 antibodies and rabbit anti-LP5 antibody Rabbit anti-LP1 and anti-LP5 antibodies were purified after rabbits were immuned with antigens isolated as described above. The purities of both antibodies were above 80% and the titer of blood serum 1:32 tested by double antibody sandwich assay.3、Development of colloidal gold immunochromatographic assay kit The size of colloidal gold particles in the kit was 25nm. The optimal concentrations for antibodies were 30μg/ml and the sensitized concentrations of NC membrane were 5 mg/ml.

主要研究工作从以下几个方面进行：1、LP1~7、9和10型多糖抗原的制备与鉴定将LP1~7、9和10型菌株分别接种在缓冲活性炭酵母琼脂培养基上，37℃、5%CO2的条件下培养，3~5天后洗下菌苔，经抽提、除蛋白、透析等步骤后得到基本无蛋白的LP多糖抗原，经紫外分光光度仪全波长扫描及Western blotting验证其抗原良好。2、兔抗LP1抗体和兔抗LP5抗体的制备与鉴定分别用LP1、LP5型多糖抗原免疫家兔，采用琼脂糖双向扩散试验检测，两种抗体血清效价均为1：32；饱和硫酸铵法提取抗体，SDS-PAGE检定其抗体纯度均达到80%以上。3、胶体金免疫层析检测试剂的初步研制采用柠檬酸三钠还原法制备约25nm大小的胶体金颗粒；分别制备兔抗LP1抗体、兔抗LP5抗体的金标探针，两种抗体的最适标记量均为30μg/ml；选择适当孔径的微孔滤膜为载体包被两种抗体，NC膜包被浓度均为5mg/ml。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。