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Anti-histidine-tag antibody is usually used for identification of expression of the recombinant protein expressed using pRSET-A. A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence.

质粒pRSET-A是一个常用的高效原核表达载体，编码一N端含组氨酸标签（6×His）的34aa前导肽序列，以方便利用抗组氨酸标签抗体鉴定或纯化所表达的重组蛋白。

A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence. The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物，经过扩增前导序列10~34aa基因序列，并重新克隆入质粒pRSET-A构建串联二聚体后，再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点，经一系列简单的酶切和连接，快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因，并成功表达其六聚体重组蛋白。

This paper, combining theory with practice, amply disusses the courses of performance of the career plans and directions of college students: to identify and locate oneself with precision

摘要从理论和实践上详细论述了大学生职业生涯规划指导的实施过程：认知自我，准确定位

This project describes amply the design processes of network middleware and the impletion processes of remote control chemical tanker operation and training software, including the middleware concept and essence of software design idea, computer program technology adopt in program-designed, network communication and so on.

本文详细讲述了网络中间件的开发过程和化学品船作业仿真培训软件的远程控制的实现过程，其中包括网络中间件的概念和本质、软件设计思想、程序设计中采用的计算机编程技术、网络通信技术等。

In the first part, a novel paraboloidal lensed multimode fiber has been analyzed amply in the coupling between the high power single emitter broad area diode laser and multimode fiber, which has been verified in the experiment. In the second part, the coupling between the high power single emitter broad area diode laser and cylindrical lensed multimode fiber has been discussed detailedly.

主要通过计算机仿真抛物面型光纤微透镜和柱状楔形光纤微透镜与大功率宽发射域半导体激光器的多模光纤耦合效率，并在实验中进行验证，提出了提高大功率宽发射域半导体激光器与多模光纤耦合效率的新方法。

The advancedness and effectiveness of the PQP(Parametric Quadratic Programming) in solving space contac problem are amply proved inpracical engineering applications.

大量实际工程应用已充分证实参数二次规划法是解决空间接触问题的一种先进的、行之有效的方法。

Prithee, friend, do not keep your knowledge to yourself; we are a large party; and any benefit which you confer upon us will be amply rewarded.

我求求你，朋友，别再自我炫耀知识了；我们是多人组成的团体，你赋予我们的那些教益将会得到无限的回报。

The paper amply introduces the logical structure and design of software and hardware of the remote multi-channel instrument system for temperature measurement. LabVIEW primary control module, cure fitting module, data acquisition module, channels and scopes set module, data processing module, data analysis and display module, data storage and redisplay module, printing module are designed in this system, which realizes the data acquisition, processing, analysis and display.

本系统采用模块化设计思想，主要分为八个模块：LabVIEW总控制模块、曲线拟合模块、数据采集模块、测温通道和测温范围设置模块、数据预处理模块、数据分析与显示模块、数据存储与回放模块、打印输出模块；实现了对环境温度信号的采集、处理、分析和显示。

A protruding rock was amply named the Little Butte that marked the halfway point between the parking lot and the large Saddleback Butte.

一座裸露的岩石名为小山，算是停车场和马鞍山的中途站。

We research amply on the denial of service attack, and get along with the source traceback.

因此，研究拒绝服务攻击及其对策是很重要的。

The big eared hedgehog, the little owl, the sand gecko, the three-toed jerboa, and every show needs a villain - saw-scale - the viper!

大耳刺猬，小猫头鹰，沙壁虎，三趾跳鼠，每场戏都需要反派角色：蝰蛇。

Plasmodiophora brassicae ; resting spores ; density gradient centrifugation of sucrose ; ultrasonic disruption

根肿；休眠孢子；蔗糖密度梯度离心；超声波破碎