查询词典 amplification

Methods: The residual lymphoblast percentage of bone marrow was evaluated on day 14 during a four-drug induction in 21 children with ALL. Telomerase activity of bone marrow cell was determined by telomere repeat amplification protocol -silver stain technique at differential clinical stages.

以多药联合化疗方案进行诱导缓解和缓解后治疗，以诱导治疗第14天的骨髓原始淋巴细胞比例作为治疗反应敏感性指标，以端粒重复序列扩增-银染色技术检测端粒酶活性作为微小残留病白血病指标。

Phage display of recombinant human lymphotoxin mutation librariesand receptor affinity screeningContraction of conformable large molecule mutation library is pivotal for molecular evolution study in vitro. In order to extend the range of the screening region of amino acid in LT gene, several receptor binding sites of LT were mutated randomly by overlap PCR amplification with the random nucleotide primers, the R46, S106, L130 combined site-directed random mutation library and R46~A52, S106~F110, R46~A52 + S106~F110 region random mutation libraries of rhLT were constructed successfully.

二。重组人淋巴毒素噬菌体库的构建及受体亲和筛选构建适合的大容量生物大分子变异体文库是进行体外分子进化研究的关键，为了扩大LT基因序列中被筛选的氨基酸范围，我们采用含随机核苷酸序列的引物，通过Overlap PCR的方法对LT的受体结合区域进行多点随机突变，分别构建了rhLT R46+S106+L130三点随机突变组合文库和R46～A52、S106～F110、R46～A52+S106～F110区域随机突变体库。

The amplification failure rate of the single cell 5-plex nest PCR is significantly associated with the difference of the methods of cell lysed. Using appropriate method to lyse cell can improve the accuracy of single cell PCR.

结果表明单细胞PCR模板制备与PCR扩增失败有密切关系，选用合适的细胞裂解方法可提高单细胞PCR的准确性。

Finally designs has the tonality control circuit the audio frequency amplification electric circuit to have very well chooses the frequency function, with pronunciation magnifying power.

最后设计出来的带有音调控制电路的音频放大电路具有很好的选频功能，和语音放大能力。

The multiple scattering which doesn＇t form loop can increase the mean free path, thus enhancing light amplification , and making laser in various directions.

不构成回路的多重光散射增加了激光在增益介质中的路程，使激光增强，并使激光沿各个方向发射。

Falciparum isolates prepared directly from 5 cases of cerebral malaria patients'blood in Mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding the regions 12—17 in MAD20 merozoite surface protein 1(MSP1) of Papua New Guinea strain of P . falciparum were used as primers. The PCR products were digested with EcoRI and KpnI, respectively, and the generated fragment regions 16—17 were cloned into M13mp18 and M13mp19 vectors and their DNA were analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应技术对中国5 例脑型疟患者恶性疟原虫云南省勐腊县勐罕CMH/YN 分离株和云南省盈江县农场CYJ/YN 分离株基因组DNA 裂殖子表面蛋白1 (MSP1)第13—17 区基因进行扩增，将扩增产物分别经EcoRI 和KpnI 双酶切后，回收的MSP1 第16—17 区基因分子定向克隆M13mp18 和M13mp19 载体，按Sanger 双脱氧链终止法进行DNA 序列测定，并与MAD20、K1 和Wellcome 株原型基因进行同源性分析比较。

Methods Genomic DNA samples of two isolated Plasmodium falciparum isolate strains prepared directly from 5 cases of cerebral malaria patients′ blood in mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding FC27 merozoite surface protein 2 (MSP2) of Papua New Guinea strain of Plasmodium falciparum were used as primers. The PCR products were digested with BamH1 and Hind Ⅲ respectively, and the generated fragment MSP2 were cloned into M13mp18 and M13mp19 vectors and their DNA was analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应对5例中国脑型疟患者恶性疟原虫云南省勐腊县勐罕分离株和云南省盈江县农场分离株基因组裂殖子表面蛋白2(MSP2)基因进行扩增，将扩增产物分别经BamHI和Hind Ⅲ双酶切后，回收的MSP2基因分子定向克隆M13mp18和M13mp19载体，按Sanger双脱氧链终止法进行DNA序列测定，并与恶性疟原虫株FC27、K1、IC1和CAMP株进行同源性分析比较。

At last, through the analysis of the T-stress, it is concluded that the T-stress plays an important role for the oriented microcrack cases. It is necessary to consider the effect of the T-stress in determining the shielding or amplification effect of the oriented microcracks.

最后，本文采用离散模型研究了各向异性材料主微裂纹干涉问题中T应力对主裂尖应力强度因子的影响：T应力对主裂尖应力强度因子的影响是不能被忽视的，这种影响不但与微裂纹的几何分布有关，而且还与T应力的大小和符号有关。

Laser capture microdissection combined with RNA linear amplification in vitro can be successfully applied to obtain pure objective cells for research. The integrity of the amplified RNA is good and can be employed in further research.

结论LCM结合RNA体外线性扩增技术能成功地获取较为单一的研究目的细胞，扩增后RNA完整性较好，能用于进一步研究中。

METHODS Microdissection technique with micromanipulator under microscope, as well as subsequent DNA extraction and polymerase chain reaction amplification methods, was employed.

通过光学显微镜直视下用显微操纵系统进行显微切割技术结合DNA提取和PCR方法，对在组织切片上获取病毒核内包涵体的来源进行分子生物学鉴定。

The changes of the HAF after liver injury may be related to the volume of blood loss, the intraabdominal pressure and the pneumoperitoneum duration.

肝撞击伤伴失血后肝动脉血流量的变化与失血量、气腹压力以及气腹持续时间等有密切联系。

Reneging on our commitments to the world's poor cannot be an austerity measure, she said.

放弃我们给世界上的穷国的承诺不能成为一种紧缩措施。