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RESULTS: Compared with NS control group, the ratio of HW/BW, LVW/BW and the content of hydroxyproline, AngⅡ, MDA and iNOS activity in the left ventricle were significantly increased. The cNOS, SOD, GSH-Px activities and NO content were obriously decreased in the ISO model group. After treatment with PNS, the left ventricular NO content, cNOS, SOD and GSH-Px activities were markedly higher than those in ISO model group. The content of MDA, AngⅡ and iNOS activities and the ratio of HW/BW, LVI were significantly lower than those in ISO model group.

结果:ISO模型组大鼠的HW/BW、LVI、左心室HyP、AngⅡ、MDA含量和诱生型NOS活性显著高于生理盐水对照组,SOD、GSH-Px及结构型NOS活性和NO含量明显比生理盐水对照组低;PNS治疗组左心室心肌组织中NO含量、cNOS、SOD和GSH-Px活性明显高于ISO模型组;MDA和AngⅡ含量及iNOS活性和心脏重量指数比ISO模型组低。

Meanwhile, ABA inhibited the activities of phospho-glucose isomerase and invertase, increased the activities of phospho glucose mutase and starch phosphorylase, indicating that ABA was the most effective factor in saccharide metabolism and to the related enzyme activities on the 1st to 7th days after treatments.

推测非还原糖含量的升高可能是ABA促进其积累和抑制转化酶活性所致,淀粉含量升高的原因可能是ABA增强了淀粉磷酸化酶的活性,果糖与葡萄糖比值的降低可能是由于ABA对PGI活性抑制和对PGM活性促进的双重影响。

Forβ-l,3-glucanase,its activity increased to peak level after 2 to 5 days and was 2.02 and 1.34 folds of check,then decreased rapidly and resumed to normal level after 5 and 9 days.Generally Psoralea corylifolia L.extract may enhance the activities of chitinase andβ-l,3-glucanase in cucumber leaves,then induce the enhancement of activities of defensive enzymes.

从所测定的几种酶活性变化时间来看,补骨脂提取物处理黄瓜幼苗后,首先,使黄瓜体内的几丁质酶和β-1,3-葡聚糖酶等病程相关蛋白活性升高,然后在两者的作用下,诱导了黄瓜体内过氧化物酶、多酚氧化酶、苯丙氨酸解氨酶等一些防御酶活性的提高。

And Cynodon dactylon reached to peak, which were 2.88 and 2.87 times of 4 h treatment respectively; but after 24 h treatment, activities of SOD of Cynodon dactylon had lower speed. The activities of POD have the same trend to activities of SOD.

POD活性的变化规律与SOD活性相同,黑麦草和狗牙根的POD活性同样在5℃处理20h时达到峰值,峰值后的POD活性减少率同样是狗牙根明显大于黑麦草。

Study of kinetic antibacterial activities of QJ2 against pathogen W-1 was tested by viable bacteria counts and absorbency at 600nm: 106~ 107 cfu/mL cells of W-1 began to decrease in 15 min after mixing with CFS(cell free-supernatant) of QJ2; the bacterial counts decreased to a minimum at 4h, and the numbers of bacteria began to increase after 6h. The cells of QJ2 did not show any sensitivity to its inhibitory substances in CFS. The size of active molecule in the CFS of QJ2 was smaller than 8000 Da by measuring the inhibitory activities of the fluid inside and outside the dialysis cellulose tubing. QJ2 was identified as Flavobacterium ordaratum by traditional morphological and biochemical method and API-20 identification system.

并对拮抗菌QJ2的拮抗作用进行了研究,结果为:QJ2有广泛的弧菌抗菌谱,对37株弧菌的抗菌阳性率达到89.2%(33/37);用活菌平板计数法和O.D.600nm研究了QJ2培养物的去细胞上清液与病原菌W-1的作用动力学,15min时W-1的细菌数便开始减少,4h时细菌数最少,6h后开始增加,而对照组的细菌数呈逐渐上升趋势;QJ2对自身的拮抗物质不敏感;QJ2抗性物质的分子量不大于8000Da;经常规生理生化方法和API-20细菌快速鉴定系统鉴定,QJ2为气味黄杆菌。

Leather goods industry, there have been the pioneer of direct marketing network, Melian, in order to build high-end leather goods for men's professional goal of leather goods brand, as a writer of magazine editors, and invited to come to Yangzhou Melian's flagship store,"Melian also pre - To do a special counter products, but in order to allow customers to get more interest, we have chosen to take the network marketing model, relying on the fashion models and excellent quality, together with the flagship store to give customers Zero experience, I believe that consumers will be able to satisfaction "Store manager told me, when I asked how to avoid buying after the return, he told me," is different from leather clothing, not size, Melian and the production process is very strict and put an end to 100% of the new Poor, Melian's shopping Web site for product configuration is also very clear not only that, we have to be registered as a member of the site, but also received regular Melian activities to inform the members and activities, the biggest benefit to the consumer feedback "Yes ah, such a high-quality products and perfect after-sales service system, the heart can not do?

皮具行业当中,也出现了网络直销的先行者,米莱恩,以打造男士高档皮具为目标的专业皮具品牌,笔者作为一个杂志的采编,受邀来到扬州米莱恩的旗舰店,&前期米莱恩也做精品专柜,但为了让客户得到更多的利益,我们选择采取网络营销的模式,依靠时尚的款型和优秀的质量,加上旗舰店给予客户零距离的体验,相信一定能够让消费者满意&店经理这样告诉我,当我问到如何来避免购买后的退换货问题时,他告诉我,&皮具不同于衣服,没有尺寸的问题,而且米莱恩的生产流程非常严格,100%杜绝新品不良的现象,米莱恩的购物网站上对于产品的配置也很清晰,不仅如此,注册成为我们的网站会员,还会定期收到米莱恩的活动通知和会员活动,把最大的实惠反馈给消费者&,是啊,如此优质的产品和完善的售后服务体系,谁能不心动呢?

The results showed that in the growth period of R. glutinosa, soil ferulic acid, vanillic acid, and vanillin contents had a persistent increase, while β-hydroxybenzoic acid content increased first but decreased then. The addition of ferulic acid (8 μg·ml-1), vanillic acid (08 μg·ml-1), vanillin (12 μg·ml-1), and β-hydroxybenzoic acid (30 μg·ml-1) in water culture had stronger inhibitory effects on R. glutinosa root length, root mass, fresh plant mass, and plant height. Treating with test phenolic acids except β-hydroxybenzoic acid increased leaf chlorophyll content significantly, compared with the control. After treated with test phenolic acids, plant SOD and POD activities decreased after an initial increase, and plant malondialdehyde content increased. In the treatment of ferulic acid, root SOD and POD activities were the lowest, and roots were rotted and dead.

结果表明:阿魏酸、香草酸和香草醛含量在地黄生长期表现为持续增加,而对羟基苯甲酸含量先升高后降低;外加阿魏酸(8 μg·ml-1)、香草酸(0.8 μg·ml-1)、香草醛(1.2 μg·ml-1)和对羟基苯甲酸(3.0 μg·ml-1)对水培地黄根长、根质量、全株鲜质量和株高有较强的抑制作用;酚酸处理使地黄叶片叶绿素含量降低,除对羟基苯甲酸外差异性极显著;经过酚酸处理的地黄幼苗SOD和POD活性为先升高后下降,丙二醛含量升高,其中阿魏酸的处理使根中酶活性降至最低,根部腐烂死亡。

From the writers who went southwards to Nanyang from the late-1920s to the early-1930s, I select three Chinese revolutionary writers as examples in the present paper. From their main experiences, important activities before going southwards and their social activities, cultural activities during exiling in Nanyang and their relative cultural activities after returning to homeland, It is not difficult to discover that using the writers who went Southwards as intermediaries, the Chinese proletarian literature movement during the 1920s-1930s promote the Nanyang " Lately Arising Literature Movement" to grow up.

本论文选取20世纪20年代末至30年代初南下南洋的三位中国革命作家洪灵菲、许杰、马宁,对他们在南下前的主要经历和重要活动,流亡南洋期间所从事的社会活动和文化活动,以及回国后所继续从事的与南洋有关的文学活动加以爬梳,从中不难发现,中国20世纪20-30年代的无产阶级革命文学运动,透过南下作家这一中介促使了南洋&新兴文学运动&的崛起,继而对当地的文学艺术、社会思想等各方面产生程度不同的影响。

The results showed:①Anthocyanin content in mature fruits was 132170 U·g-1FW in bud mutation which was very significantly higher than that in'Ralls'(49140 U·g-1FW);②Activities of PAL and UFGT were almost the same between the two materials, but CH I and DFR activitieswere significantly high in bud mutation, which were related to the increase of anthocyanin levels in the mutant;③Bagging treatment inhibited anthocyanin synthesis of bud mutation. But anthocyanin content had significantly increased after removing the bags. Activities of CHI and UFGT increased rap idly and were much higher than those in the control after the bag removal. The increase was consistentwith the imp rovement of anthocyanin levels.

结果显示:①在果实发育期间,芽变果皮的花青苷含量明显高于'国光',尤其是成熟期芽变果皮花青苷含量为132170 U·g-1FW,而'国光'仅为49140 U·g-1FW;②在果实发育期间,两个品种间PAL 和UFGT的酶活性无明显差异,但芽变的CHI和DFR酶活性明显高于'国光',表明芽变花青苷合成能力的提高与CHI和DFR酶活性高有关;③套袋抑制芽变果皮花青苷的合成,但解袋后花青苷的含量极显著升高,解袋后4种酶的变化趋势差异较大, CHI和UFGT活性均迅速升高,明显高于对照,这与解袋后花青苷迅速合成相吻合。

Huang-hua' pear fruit was a typical climacteric fruit after harvest, and the fruit senescence-related factors, such as LOX activity, 02" content, AOS activity, ACC synthase activity, ACC content, ACC oxidase activity and ethylene production, changed with peaks at 20 "C. The activities of LOX, AOS, ACC synthase, ACC oxidase and the accumulation of O2 ", ACC content and ethylene synthesis were inhibited when stored at 1癈. And the severity of flesh floss and fruit decay was lightened. Low temperature combined with polyethylene film bag packing (PEF1 and PEF2) could further decrease the rate of weight loss, flesh floss, and fruit decay and keep preferable freshness. When PEF1 and PEF2 packing fruits were transferred to shelf life at 20 "C after storage at 1癈. The activities of LOX, AOS, ACC synthase, ACC oxidase could be suppressed and the accumulation of 02 , ACC content and ethylene production were slowed down to a certain extent, especially during the shelf life after 60 d storage. PEF1 and PEF2 packing fruit could maintain higher firmness as well, and the effects became more distinct along with the storage period, there existed no significant difference between PEF1 and PEF2 treatments.

试验结果表明,在20℃下,LOX活性、O2-水平、AOS活性、ACC合成酶活性、ACC含量、ACC氧化酶活性及乙烯的生成,均呈峰形变化;1℃处理可抑制LOX、AOS、ACC合成酶、ACC氧化酶的活性和O2-、ACC的积累,抑制贮藏果实的乙烯合成,减轻果肉发绵和果实腐烂。1℃下贮藏的果实,若结合不同聚乙烯薄膜袋(PEF1和PEF2)小包装,可以进一步降低果肉发绵率和果实腐烂率,保持果实新鲜度;当果实经过一段时间贮藏后,转移到20℃下,在货架期间,PEF1和PEF2对果实成熟衰老各个相关因子均有不同程度的抑制效应,主要表现在贮藏60d后的货架期间,同时维持较高的果实硬度,对硬度的影响随着贮藏时间的延长愈加明显,PEF1和PEF2处理效果之间无显著差异。

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