查询词典 actin

In this thesis, we studied the effects of nuclear actin on gene transcription regulation, with a special focus on the relations between the nuclear actin and RNA polymerase II in concanavalin A-stimulated lymphocytes and HeLa cells.

本文以ConA激活的淋巴细胞和HeLa细胞为实验材料，从不同角度研究了细胞核肌动蛋白与RNA聚合酶Ⅱ相互作用，并对细胞核肌动蛋白参与RNA聚合酶Ⅱ的作用机制进行了探讨。

Sequence analysis reveals the identity rate of β-actin cDNA nucleotide sequence between the rice field eel and Tilapiamossambica, Salmo salar, Oryzias latipes, Oncorhynchus mykiss, Danio rerio, Carassius auratus, Physalaemus pustulosus, Bos taurus, Gall us gallus, Mus musculus, Hoino sapiens are 68%~95%; and the similarity of 3-actin amino acid sequence is 97%~100%.

将所得序列与各科鱼、蛙、鸡、牛、鼠、人等的β-肌动蛋白基因序列同源性分析显示，核苷酸序列具有68%~95%的相似性，氨基酸序列具有97%~100%相似性，显示该基因在生物进化过程中的保守；系统发育分析表明黄鳝β-肌动蛋白基因与罗非鱼的亲缘关系最近。

After developing 48 hours, hepatocytes enters 2.0 mM t-butyl hydroperxide or 2.0 mM diamide to induced cell blebbing. The samples collected at 0, 15, 30, 60 minutes to analyze cell about membrane protein thiol deletion; the leakage of lactate dehydrodenase; the change of glutathione; the lipid peroxidation; the variation of actin and tubulin, and use Confocal fluorescent microscope to observe the change of actin and tubulin in rat hepatocytes.

肝细胞在48小时培养后，分别加入2.0mM t-buty1 hydroperoxide或2.0 mM diamide诱发细胞膜小泡生成，并分别於0、15、30、60分钟取样，分析细胞膜硫醇流失程度；乳酸去氢□渗漏；□胱甘□浓度变化；脂质过氧化；肌动蛋白与管蛋白变化；并利用雷射共轭焦萤光显微镜观察肝细胞中肌动蛋白及管蛋白的变化。

The result indicates the t-BHP or diamide both cause the mass loss about the membrane protein thiol in hepatocytes, the fast oxidization about glutathione in hepatocytes, the leakage of lactate dehydrodenase, but the level of t-BHP is larger than of diamide. In the aspect of lipid peroxidation, only the t-BHP produces apparent change. As to actin and tubulin, they both find fall-off and present time-dependency effects. The result in Confocal fluorescent microscope also show these two cytoskeletal proteins are related to cell blebbing, but they don''t play the same role during the porcess. Tubulin may be more important than actin. If the study predispose dithiothreitol before adding t-BHP or diamide, then the damage by the diamide is repressed, but just partly protection for the damage by t-BHP.

结果显示t-BHP或diamide均可造成细胞膜蛋白硫醇大量流失与胞内GSH快速氧化，亦显著导致乳酸去氢□渗漏，但t-BHP诱发的渗漏程度大於diamide；在脂质过氧化方面，则仅t-BHP处理组产生明显的脂质氧化情形；至於肌动蛋白与管蛋白单体均可见减少且呈现时间依赖效应；在雷射共轭焦萤光显微镜观察中，亦显现这两种架构蛋白与细胞膜起泡有关，但两者在小泡生成的过程中扮演的角色并不完成相同，可能管蛋白较肌动蛋白重要；如果细胞在t-BHP或diamide加入前预先以dithiothreitol处理，则diamide 所造成的伤害显著的受到抑制，但DTT对t-BHP所诱发的伤害则仅有部分保护效果。

Results: MC of tryptase immunoreactivity contain abundant F-actin ring that region of cytomembrane endothecium form a barrier of blocking tryptase liberation. Copious tryptase temporarily was stored in secretory vacuole, and small amounts tryptase was released from secretory vacuole for depolymerize of F-actin ring in cell.

结果：类胰蛋白酶免疫反应性的肥大细胞内含有丰富的丝状肌动蛋白环，在质膜内层区域形成阻碍类胰蛋白酶释放的屏障；大量的类胰蛋白酶暂存于分泌泡中，少量的类胰蛋白酶因细胞内丝状肌动蛋白环的解聚使之从分泌泡中释放。

Results: MC of tryptase immunoreactivity MC(subscript T contain abundant F-actin ring that region of cytomembrane endothecium form a barrier of blocking tryptase liberation. Copious tryptase was temporarily stored in secretory vacuole, and small amounts of tryptase was released from secretory vacuole for depolymerize of F-actin ring in cell.

结果：类胰蛋白酶免疫反应性的肥大细胞内含有丰富的丝状肌动蛋白环，在质膜内层区域形成阻碍类胰蛋白酶释放的屏障；大量的类胰蛋白酶暂存于分泌泡中，少量的类胰蛋白酶因细胞内丝状肌动蛋白环的解聚使之从分泌泡中释放。

The amount of vimentin IF per cell was higher than those of tubulin and F-actin at 12-24h of culture, but it broken down and decreased steadily when the cells became differentiated into late erythroblast at 36-48h of culture, thus facilitated the eccentric nucleation which we regard it as the initial step of denucleation. Both of the fluorescence intensity of tubulin and actin exhibited a significant rise and aggregated between the extruding nucleus and incipient reticulocyte before and during denucleation, and finally play a role in the commitent to ennucleation, the second phase of denucleation.

在培养24小时前，每个细胞中波形纤维含量均高于同期微丝和微管蛋白含量，但在培养24-36小时的晚幼红细胞阶段，波形纤维蛋白降减和解聚，为核偏位创造了条件，这是排核的第一阶段，而随后微丝和微管蛋白的荧光染色强度则有所增加，并逐渐聚集在外排中的核与预期形成网织红细胞之间的缢痕区，在将细胞核排出的排核第二阶段起主要作用。

RESULTS: A total of 36 rabbit models, 6 in the 1-month therapy group and 8 in the 3-month therapy group were died from infection. In the 1-month therapy group, thick ureteric wall and smooth ureteric lumina developed without any contracture. Under the optical microscope, transitional epithelial cells covered throughout the inner walls and smooth muscle cells appeared sparsely in a mussy configuration. In addition, CKAE1/AE3 staining was positive butα-actin staining was very weak when checked by immunohistochemistry. In the 3-month therapy group, amnion-ureters were enclosed by their peripheral tissue and showed rich blood vessels and normal ureteric lumina without significant contracture, futher more, there had formed a well-arranged transitional epithelial layer and smooth muscular layer, the immunohistochemistry result showed that the expression of CKAE1/AE3 and α-actin were positive.

结果：36只大白兔中，1个月治疗组死亡6只，3个月治疗组死亡8只，死亡原因均为感染。1个月治疗组大白兔羊膜输尿管柔软，长4 cm，管壁变厚，无挛缩，移行上皮组织爬满内壁，平滑肌组织部分再生，但较稀疏，排列不规则；免疫组织化学检查提示CKAE1/AE3染色阳性，α-Actin染色微弱。3个月治疗组大白兔羊膜输尿管与周围粘连，有丰富的血运，无明显挛缩，管腔不缩小，已形成良好的移行上皮层和平滑肌层，免疫组织化学显示α-Actin和CKAE1/AE3染色均为阳性。

In chapter two, the structures of myosin and actin, the mechanochemical actin-actived myosin ATPase cycle are introduced.

第三章主要介绍肌肉收缩的一些实验结果和已有的理论模型。

Cancellous bone from the lumbar proteomic expression analysis show that actin, keratin, enolase, ATP synthase, the expression of myosin may be related to ovarian lumbar cancellous bone after osteoporosis-related happened; Qiang Gu Bao Po on the lumbar spine osteoporosis intervention effect may be related to reduced carbonic anhydrase, actin, crystal protein, 3- phosphoglycerate dehydrogenase, serum albumin, ATP synthase, myosin, the enolase expression.

从腰椎松质骨蛋白质组表达分析可知，肌动蛋白、角蛋白、烯醇化酶、ATP合酶、肌球蛋白的表达增强可能与去卵巢后腰椎松质骨骨质疏松的发生相关；强骨宝对腰椎骨质疏松的干预效应可能与下调碳酸酐酶、肌动蛋白、αB-晶体蛋白、3-磷酸甘油醛脱氢酶、血清白蛋白、ATP合酶、肌球蛋白、烯醇化酶的表达有关。

In this new architectural plan the Garden of Golgotha came to be at the centre of the new city.

Aelia 是高举他自己，并在此城内朝拜罗马诸神。

The continuous nature of peat accumulation means that terrestrial coaly rocks may provide a higher resolution and more complete record of base-level change than the time-equivalent shallow-marine strata, thus enabling the identification of highfrequency cycles that are not recorded in themarine strata.

泥炭积累的持续性质的手段，陆地coaly岩石可提供较高的分辨率和更完整的记录基准面变化的影响时，相当于浅海阶层，从而使确定的高频周期，不记录在themarine 阶层。