查询词典 TOF

Study on the Crystallization Process of Labeled GL-7-ACA Acylase CA130 Complex 7β-bromoacetyl amino cephalosporanic acid (BA-7-ACA), an analog of glutaryl-7-amino cephalosporanic acid (GL-7-ACA), can inhibit and specifically alkylate GL-7-ACA acylase (CA130) from Pseudomonas sp. 130, forming a carbon-carbon bond between BA-7-ACA and the C-2 on indole ring of Trp-β4 residue of CA130.Here we reported that BA-7-ACA labeled CA130 (BA-C130) could self-catalyze the hydrolysis of BA-7-ACA during crystallization process. The hydrolysis was confirmed to be a reaction analogous to the one of GL-7-ACA by comparative MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) spectrometry analysis.

二、GL-7-AcA酰化酶CA130标记复合物的结晶过程研究溴乙酰氨基头孢烷酸（7β-bromoacetyl amino cephalosporanic acid，BA-7-ACA）作为戊二酰-7-氨基头孢烷酸（GL-7-ACA）的类似物，不仅能够抑制GL-7-ACA酰化酶CA130的活力，而且能通过在BA-7-ACA和CA130的β亚基第四位色氨酸吲哚环二位碳原子之间形成碳-碳共价键而将CA130特异的烷基化。

The relationship of the 7th cranial nerve and adjacent vessels was best visualized on the 3D TOF sequence and constructive interference in steady state sequence. The results were compared with the outcome of operation blinded.

HFS患者52例和健康成人20例进行MRTA检查，观察3D-TOF成像序列结合3D-CISS序列中脑桥小脑角池内面神经与周围血管的关系，并与手术结果进行盲法对照。

Several coterminous bands could be observed by PAGE of natural Eisenin Ⅰ and several coterminous peaks of Eisenin Ⅰ were also detected with MALDI-TOF-MS when the molecular weight of three main peaks are 24, 645, 25, 052 and 25, 281, separately. The amino acid composition of Eisenin Ⅰ(pI. 8) is specilized with high content of acidic amino acids and low content of alkaline amino acids; At the same time, the content of Ser and Thr are quite high and that of Lys, Met, Phe and Trp are quite low. Eisenin Ⅰ is highly homologous with serine proteases. Then, by fibrin plate assay, Eisenin Ⅰ was identified to be a plasmin and also a plasminogen activator, and the fibrinolytic activity was inhibited by PMSF (an inhibitor of serine proteases).

赤子素Ⅰ氨基酸组成特点为高的酸性氨基酸和低的碱性氨基酸含量，且Ser、Thr含量高而Lys、Met、Phe和Trp含量很低；等电聚焦电泳测得其等电点pI.8.N端序列比较结果显示赤子素Ⅰ与丝氨酸蛋白酶类高度同源，纤维蛋白平板法测得赤子素Ⅰ同时具有纤溶酶和纤溶酶激酶活性；通过PMSF对赤子素Ⅰ纤溶酶活性的抑制实验证明，赤子素Ⅰ属于丝氨酸蛋白酶类。

The model of a collection of illustrative plates of serum protein dactylogram that conclusion builds can distinguish uremic patient and health person, the respect such as the filtration of the SELDI-TOF-MS diagnosis in uremia and element of mark of particularity protein biology has fair value.

结论建立的血清蛋白质指纹图谱模型能够区分尿毒症患者和健康者，SELDI-TOF-MS在尿毒症的诊断及特异性蛋白质生物标志分子的筛选等方面具有一定价值。

Methods Proteins and peptides in CSF were separated by two-dimensional gel electrophoresis（2DE） and ultra-performance liquid chromatographer and identified by matrix assisted laser desorption ionization time of flight mass spectrometry（MALDI-TOF-MS） and electrospray ionization to study differential proteomic expression in the cerebrospinal fluid between patients with Demy- elinative disease and with other neurological diseases.

方法以中枢神经系统脱髓鞘疾病患者的脑脊液为研究对象，分别采用固相pH梯度双向凝胶电泳分离总蛋白质，凝胶经银染显色后，用图像分析软件进行比较分析、识别差异表达的蛋白质，离线应用基质辅助激光解析电离飞行时间质谱得到相应的肽指纹图谱；同时用超高压液相色谱分离酶解肽段，在线采用电喷雾四极杆飞行时间质谱鉴定肽段氨基酸序列；搜索数据库鉴定部分蛋白质。

Results Of total 11 patients, 10 cases were mirror-image dextrocardia, 1 case was dextroversion. Among 10 patients were diagnosed as mirror-image dextrocardia, 5 patients were found to have concomitant cardiovascular malformations, the major anomalies were VSD,TOF, DORV. 1 case of dextroversion with complex cardiac anomalies: CECD+SA+DORV+PS. Conclusion Congenital dextrocardia may present with complex anomalies.

结果：11例先天性右位心患者中，镜像右位心10例，右旋心1例。10例镜像右位心中5例合并心内畸形，主要畸形为：室间隔缺损2例，法洛四联症2例，右室双出口1例；超声漏诊2例合并双上腔静脉。1例右旋心合并复杂畸形：完全型心内膜垫缺损、单心房、右室双出口、肺动脉瓣重度狭窄。

The molecular weights of the 11 major bands were 82, 79, 60, 51, 46, 38, 32, 30, 28, 24 and 18kD, respectively. The results of Western blotting with the positive serum of anaphylactic patients showed that the 1st-4th instar larvae all had the specific allergens whose molecular weights were 82 and 79kD, respectively; only the 5th instar larvae all had the specific allergen of 30kD. We purified the protein of 30kD by ion exchange chromatography and gel isolation and identified it as ectoblast protein by MALDI-TOF-MS.

选用家蚕过敏患者阳性血清进行免疫印迹，1~4龄家蚕均显示出82和79kD的特异性变应原；但只有5龄家蚕的30kD蛋白为特异性变应原，通过离子交换层析和经切胶纯化出30kD蛋白，再经MALDI-TOF-MS鉴定该蛋白为外膜蛋白。

Centrifuge blood at 4℃and take supernatant,which was preserved in frigidaire at-70℃.(2)Serum protein mass peak were detected by SELDI-TOF-MS and weak canonic chips.

研究方法1。检测宫颈癌早期和中晚期差异蛋白质的表达(1)样品收集：宫颈癌患者共44例，临床分期为早期26例，中晚期18例。

Compared to TOF-MRA, 3D contrast-enhanced MRA is useful for visualization by subtraction technique of malformation components presented by hematoma or by haem product.

与TOF-MRA相比，三维对比增强磁共振血管造影通过减影技术将由血肿或血红素形成的伪畸形病灶消除，因而更益于显影。

Compared to TOF-MRA, 3D contrast-enhanced MRA is useful for isualization by subtraction technique of malformation components presented by hematoma or by haem product.

与TOF-MRA相比，三维对比增强磁共振血管造影通过减影技术将由血肿或血红素形成的伪畸形病灶消除，因而更益于显影。

We stopped by the old fashion ice cream parlor Manhattan Beach Creamery for some refreshment. The "No Shoes, No Shirt, No Problem" sign on the wall epitomizes the beach lifestyle.

我们去商店区的一家老式冰淇淋店坐坐，墙上挂著「没鞋，没上衣，没问题」的牌子完全捕捉了海滩生活的随性及风格。

"If therefore", argues Bellarmine we can offer our prayers and our satisfactions in behalf of those detained in purgatory, because we are members of the great body of Christ, why may not the Vicar of Christ apply to the same souls the superabundant satisfaction of Christ and his saints--of which he is the dispenser?

" "因此，如果"，认为贝拉明（德indulgentiis ，十四）"我们可以提供我们的祈祷和我们满意的代表那些被拘留在炼狱，因为我们是成员的伟大基督的奥体，为什麼可能不是牧师基督适用於同样的灵魂满意的过剩是耶稣和他的圣人-他是在配药？