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According to our method, we can quantify the total amount of isoflavone more rapid and conventiently without preparing all 12 kinds of standards.In this study, acetyl- and malonyl- glycosides were converted to 7-O-glycosides by reacting with 1M methanolic sodium methylate at 60℃ for 20 min. Then, the solution was neutralized with 2.5N HCL and extracted for further 30 min at 60℃. Aglycones and 7-O-glycosides were then analysed by HPLC.

根据本研究，此方法系使用1.0 M sodium methylate在60℃下与样品中之acetylglycoside、malonylglycoside反应20分钟，将其转化为7-O-glycoside，反应完成后以2.5N盐酸水溶液中和，调整溶液为80%甲醇溶液并在60℃下继续萃取30分钟然后进行HPLC分析；由於仅需要分析两种形式的大豆异黄酮化合物（aglycones及7-O-glycosides），因此可以有效地缩短分析时间至30分钟以内。

The results got from 5 L fermentation tank tests showed that the optimal culture conditions were compound culture,30℃,pH 5.0,300 r/min,air flow 4 L/min and fermentation periods 24-27 h,and the maximal concentrations of live microzyme and lactobacillus were 2.00×108,2.55×109 individuals/mL in the fermentation liquid,respectively.

通过5 L发酵罐上罐试验确定秸秆发酵的最佳培养条件为：培养基合成，温度30℃，pH 5.0，搅拌速度300 r/min，通气量4 L/min，发酵周期2427 h，酵母菌和乳酸菌的最大菌液浓度分别可达2.00×108，2.55×109个/mL。

Results The duration of operation was 90~240 min (mean,120 min).

结果 手术时间90～240 min，平均手术时间120 min。

Hypothesis in 0 ~ 10 speed range between 2000 and r/min speed error Δ requirement f r/min, more than 1 and measuring time, time than 100 ms t take 3 muon s acuities error Δ than eight mu t s, according to the formula (1) and (3) and assumptions, and computer programming.

假设转速范围在0～10 000 r/ min 之间，要求转速误差Δf ≤1 r/ min ，并且测量时间T ≤100 ms ，时间误差取3μs ≤ΔT ≤8μs ，根据公式(1)和(3)及假设条件，进行计算机编程求解。

Within 15 min after administrationof FF all subjects complained of a transient myalgia, whichdisappeared within 30 min.

在注射 FF15min 后，所有病例均诉暂时肌痛，30min 后消失。

At water temperature of 16.0-17.2 ℃, the process of embryonic development could be divided into 24 stages, which were described as follow: fertilized egg at 0 min, 2-cell stage at 75 min post fertilization; myotome forming stage at 43 h PF, and hatching-out stage at 94 h PF when the accumulative temperature reached 1616.8 ℃.h, and the average size of larvae was about 3.8 mm in body length.

梭鲈胚胎发育过程可分为24期，在水温16.0~17.2℃范围内，受精75 min开始第1次卵裂，受精后43 h开始形成器官，受精后约94 h开始出膜；刚出膜的仔鱼全长平均为3.8 mm；从受精到孵化出膜总积温为1 616.8℃。h。

In addition, Immune nephelometry assay was imployed to analysis the level of rats' plasmic Fg, in the mean time, Platelet aggregation meter was used in observing the ratio of rats' platelet aggregation at 3 min. and 10 min, respectively.

实验采用酶联免疫吸附法测定各组大鼠血浆中TXB2、6-Ke-PGF1α、PGE2的水平；另外，采用免疫比浊法测定两组大鼠血浆中纤维蛋白原的含量，并同时观察各组大鼠3min，10min内的血小板聚集率。

The optimum dyeing conditions were shown: viral samples fixed with 0.1% paraformaldehyde for 30 min at 4℃, stained with SYBR Green I 1 × 10^(-4 commercial concentration for 10 min at 80℃.

杆状病毒最佳染色条件：质量分数为0.1%的多聚甲醛固定病毒30min后，加入一定量的SYBR Green I在80℃下孵育10min。

objective to investigate the paregoric effects of butorphanol when the errhysis occurs inside the epidural catheter during continuous epidural anesthesia.methods 60 cases of kidney stones received elective surgery.during this surgery they underwent continuous epidural anesthesia and eight cases of them showed the errhysis in the epidural catheter.then epidural injection of butorphanol was given for analgesia and hr,map,spo2 as well as muscle relaxation conditions were monitored before and 5,10,20 min after injection.the conscious and breathing of patients were also monitored.results before and 5,10,20 min after injection of butorphanol,hr and map difference were not statistically significant.the analgesic results were satisfactory.conclusion butorphanol has a definite analgesic effect when the errhysis occurs in epidural catheter during continuous epidural anesthesia.

目的 探讨在连续硬膜外麻醉中，硬膜外导管内渗血时，应用布托啡诺镇痛的效果。方法择期行肾结石手术患者60例，行连续硬膜外麻醉，其中8例硬膜外导管内渗血，均经硬膜外腔注入布托啡诺镇痛，分别监测注入前及注入后5 、10 、20 min，hr、map、spo2及肌肉松驰情况，同时监测患者神志及呼吸情况。结果注射布托啡诺前及注射后5、10、20 min，hr、map差异无统计学意义，镇痛效果满意。结论在连续硬膜外麻醉中硬膜外导管渗血时，注入布托啡诺维持麻醉镇痛效果明确。

Ningnanmycin could passivate TMV during a very short time, but the passivation effect declines with increasing time. A relatively full protein content level was maintained after 10 min treatment of TMV with Ningnanmycin, but a lot of proteins were lost after 30 min treatment.

宁南霉素对TMV病毒短时间内具有钝化作用，而较长时间的作用效果不同，10 min钝化样品的蛋白组分保持较为完整，而30 min钝化样品的多肽缺失较为严重。

It's bell can reach over a meter in diameter.

它的身体最大可以达到直径1米多。

Now they are overdue and I shall have to pay a fine.

现在他们过期了，我不得不付罚款。