查询词典 Gish

In this study, the author used GISH technology to analyze the material that was resistant to take-all in test, and got three chromosome arm addition lines that carried the resistance genes derived from the amphiploid of Triticum-Haynaldia. The above arm addition lines and their resistant and sensitive parents were analyzed by RAPD method and two specific molecular markers that were tightly linked to resistant gene were obtained.

本研究先用基因组原位杂交对经鉴定抗全蚀病的材料进行分析，检测出三个抗性源于簇毛麦的端体异附加系材料，再利用RAPD方法，对这三个材料和抗感病亲本进行分析，得到了两个与全蚀病抗性基因紧密相关的特异性RAPD标记。

The chromosome constitution on H96276-2, which is a stable line derived from a sterile wheat-Agropyron intermedium partial amphiploid Zhong 3 cross to common wheat Hui 75, was analyzed by sequential GISH and FISH.

利用顺序基因组-重复序列原位杂交技术对1个来自中3不育系和普通小麦恢75杂种后代稳定株系H96276-2的染色体组成进行了分析。

The chromosme constitution on H96276-2, which is a stable line derived from a sterile wheat-Agropyron intermedium partial amphiploid Zhong 3 cross to common wheat Hui 75, was analyzed by sequential GISH and FISH.

利用顺序基因组-重复序列荧光原位杂交技术对1个来自中3不育系和普通小麦恢75杂种后代稳定株系H96276-2的染色体组成进行了分析。

The yield, quality, resistance and chromosome characteristics of the hybrids were analyzed, and the improvement of the grasses using gene introgression, androgenesis and genetic modification methods was introduced. The latest advance in GISH, genetic map construction and QTL of Festulolium hybrids were described here.

本文总结了黑麦草羊茅杂种高产、优质、抗性强的优势；分析了杂种染色体配型特点；重点介绍了通过基因渗入、雄核发育和转基因手段改良牧草的方法；对黑麦草羊茅杂种基因组原位杂交、分子标记和遗传图谱建立、数量性状位点分析等分子遗传学的最新研究进展进行了综述。

Only one complete corollinae chromosome was detected in Ml4, and was determined corollinae chromosome 9. We have used in situ hybridization of genomic DNA to discrimination the parental chromosomes in Beta Ml 4, suppression of cross-hybridization by blocking DNA was not necessary indicating that the investigated Beta genome contain sufficient species-species DNA enabling the unequivocal determination of genomic composition of the hybrids.In our study , the results of BAC -FISH combined with the analysis of GISH in Beta M14,indicating that present of the corollinae chromosome 9 alone was sufficient to confer apospory in Beta M14 upon celluar and molecular..

BAC克隆16-M11和26-L15携带M14花期特异表达的基因；在M14甜菜对应的有性栽培甜菜染色体上没有出现信号，呈半合子状态；包含的单或低拷贝DNA序列在野生白花甜菜进化过程是共线的；两个BAC克隆部分同源；位于末端重组抑制区，所有这些不同寻常的特性都暗示着：BAC克隆16-M11和26-L15和无融合生殖特性相关，可作为无融合生殖

The Brassica, being the most important genus in cruciferae, consists of a lot of species.

本文利用FISH和GISH技术，对芸薹属重要物种亲缘关系进行了研究。

Thus, the application of GISH for conifer hybrid genome studies is limited unless improvements are made in the technique.

这些情况表明，高山松虽然在基因组构成上与两个亲本基本一致，但基因在染色体组的空间排布上是存在差异的，这一点可以从rDNA-FISH中证明。

According to the number and behavior of B genomechromosomes, all hybrid plants were classified into three types. All somatic cells of TypesⅠplants contained 16 B genome chromosomes which formed 8Ⅱat diakinesis and shown8:8 segregation at anaphase; TypesⅡcontained 14 B genome chromosomes whichformed 6Ⅱ+2Ⅰat diakinesis and showed 6:8 or 7:7 segregation at anaphase. TypesⅢcontained above two chromosomes number and behavior.

同时根据B基因组DNA为探针的GISH结果，以细胞中B基因组染色体的数目及减数分裂时的配对及分离的方式，将所有杂种植株分为三大类：Ⅰ类具有16条B基因组染色体，以8Ⅱ的方式配对，以8：8分离，共32株；Ⅱ类具有14条B基因组染色体，以6Ⅱ+2Ⅰ方式配对，以7：7或者6：8的方式分离，共4株：Ⅲ类植株包含有上述两种细胞类型类型，共1株。

In this research , by using of genomic in situ hybridization , random amplified polymorphism DNA technology and three specific SCAR molecular markers, the author analyzed wheat hybrid generations carrying the desired genes that were resistant to take-all, BYDV and/or powdery mildew, and developed some alien chromosome addition and translocation lines that were important in breeding practice, and some specific molecular markers that could be applied in marker-assisted selection.

本研究运用GISH、RAPD和SCAR分析方法，对抗全蚀病、黄矮病和兼抗黄矮病白粉病的小麦杂交后代材料进行分析，获得了一些对育种实践有重要意义的易位系、异附加系材料和几个可用于辅助育种的特异性分子标记。

N9436-1, with multi-spikelet, was immune to powdery mildew. GISH and C-banding showed that N9436-1 was wheat-rye 1R disomic additional line.

结果表明，N9436-1形态学和细胞学稳定，2n=44=22Ⅱ，对白粉病免疫，携带奥地利黑麦的多小穗性状。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。