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In this paper, firstly, the embeddability of near-triangular graphs on the orientable surface is studied. By using Petersen's Theorem about 1-factor, we first prove that for a near-triangulation on the orientable surface, its geometry dual graph has a 1-factor; then after a procedure of operation leaded by the 1-factor, we show that if a graph G triangulates some orientable surface S_g, then G has a near-triangular embedding in S_h for h = g,g + 1,...,β(G/2」, where β is the Betti number of G. Hence we obtain a conclusion: A near-triangulation of the orientable surface is upper-embeddable. As a generalization, a class of near-quadrangulation is studied, and similar results are obtained.

本文首先研究了近三角剖分图的可定向曲面嵌入性质，通过运用Petersen关于1-因子的定理，首先证明了对于可定向曲面上的三角剖分图，其几何对偶图具有1-因子；然后在1-因子的导向下，通过做一系列增加亏格的手术，证明了如果一个图G三角剖分可定向曲面S_g，那么G可以近三角剖分可定向曲面S_h，这里h=g，g+1，…，「β(G／2」，β是指图G的Betti数，从而得出推论：可定向曲面上的三角剖分图是上可嵌入的，作为推广，又研究了一类近四角剖分图的可定向曲面嵌入性质，并得到类似的结论。

We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系：胶体金颗粒的大小为20nm；抗体与胶体金溶液结合的最佳pH约为8.2；最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL，抗重组Stx2B IgG为60μg/mL；最佳稳定剂为BSA；最佳缓冲液为pH8.2硼酸溶液；最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液；NC膜的封闭液为3%BSA的0.01mol/L PBST；Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg，其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg，结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

Studied on sensitivities of C. ellipsoidea to kanamycin , Chloromycetin , Hygromycin , Streptomycin and Geneticin (G418), the results showed that C. ellipsoidea is not sensitive to Km, Str and Cm, 600μg/ml km, 600μg/ml Str and 200μg/ml Cm can not inhabited the growth of C. ellipsoidea. However, it is fairly sensitive to Hm, 100μg/ml can completely stop the growth of C. ellipsoidea on solid media. We also found that C. ellipsoidea was highly sensitive to G418, 30μg/ml and 15μg/ml can inhabit the growth of C.

通过5种基因工程中常用抗生素对小球藻生长抑制的研究发现：小球藻对卡那霉素、链霉素、氯霉素不敏感。600μg/ml的卡那霉素、600μg/ml的链霉素和200μg/ml的氯霉素仍不能抑制小球藻的生长；小球藻对潮霉素较为敏感，100μg/ml的用量可抑制固体培养中小球藻的生长；而对G418表现出高度敏感性，30μg/ml的G418即可完全抑制固体培养中小球藻的生长，15μg/ml的G418即可完全抑制液体培养中小球藻的生长。

According to the amount of alive cells and the rate of conidium production, the optimum parameters were obtained as follows: bran 100 g, mineral matter M-1 15 g, K2HPO4 0.3 g, NaCl 0.25 g, KNO3 0.1 g, CaCO3 0.8 g, natural pH, liquid seed age 84 h, inoculum volume 10%, fermentation temperature 30°C, the ratio of material to water is 10:6, fermentation time 60 h.

获得固体发酵物料的最佳参数为：麸皮100 g、M-1 15 g、磷酸氢二钾0.3 g、氯化钠0.25 g、硝酸钾0.1 g、碳酸钙0.8 g，自然pH值，液体菌种种龄84 h，接种量10%，发酵温度30°C，料水比10：6，发酵时间60 h。

Let F=LF be a s-closed local formation such that every minimal non-F-groups is solvable. It is proved that 1 If every cyclic subgroup of of order 4 and every minimal subgroup of G are contained in, then G is a F-group. 2 If there exists a normal subgroup N of G such that G/N∈F and every cyclic subgroup of N of order 4 is weakly c-normal in G and every element of N of prime order is contained in Z(superscript f subscript ∞), then G is a F-group.

设F=LF是一个子群闭的局部群系，满足每个极小非F-群是可解群，证明了：1如果G的任意极小子群和任意4阶循环子群都含于Z中，那么G是F-群；2如果存在G的正规子群，使得G/N∈F，且N的任一4阶循环子群在G中弱c-正规，N的任一素数阶元含于Z中，那么G是F-群。

The production medium was optimized both by "one-variable-at-a-time" approach and the statistical methods based on Response Surface Method. The optimized medium composition was as follows: soluble starch 65.45 g/L, glucose 9.30 g/L, soybean flour 10 g/L, peptone 5 g/L, fish flour 10 g/L, yeast extract 3 g/L,(NH_4)SO_4 1.5 g/L, MgSO_4·7H_2O 4.93 g/L, KH_2PO_4 0.2 g/L, NaNO_3 0.75 g/L, CaCO_

通过单因素试验和以响应面为主的统计学方法对发酵培养基进行了优化，优化后的发酵培养基组成为：可溶性淀粉65.45g／L，葡萄糖9.30g／L，豆饼粉10g／L，蛋白胨5g／L，鱼粉10 g／L，酵母粉3 g／L，(NH_4)_2SO_4 1.5g／L，MgSO_4·7H_2O 4.93g／L，KH_2PO_4 0.2g／L，NaNO_30.75g／L，CaCO_34g／L。

In this paper, the composition of zinc phosphating solution used at environmental temperature was researched. The composition is as followings: phosphoric acid 100 mL, monometallic sodium orthophosphate 20 g/L, zinc oxide 25 g/L, bluestone 1 g/L, sodium molybdate 1 g/L, sodium fluoride 1 g/L, tartaric acid 1 g/L.

研究了配制锌系磷化液配方：1 L溶液中加入磷酸100 mL，磷酸二氢钠20 g，氧化锌25 g，硫酸铜1 g，钼酸钠1 g，氟化钠1 g，酒石酸1 g。

Degree sum conditions of IM-extendable graphs are researched. The main results are as follows:(1) Let G be a graph with 2n vertices. If for each pair of nonadjacent vertices u and v in G,, then G is IM-extendable.(2) Let G be a claw-free graph with 2n vertices. If for each pair of nonadjacent vertices u and v in G,d+d≥2n+3, then G is IM-extendable.

研究导出匹配可扩图的度和条件，主要结果如下：(1)若图G有2n个顶点，且对于G中每一对不相邻的顶点u和v，，则G是导出匹配可扩的；(2)若G是一个有2n个顶点的无爪图，且对于G中每一对不相邻的顶点u和v，d+d≥2n+3，则G是导出匹配可扩的。

Degree sum conditions of IM-extendable graphs are researched. The main results are as follows 1 Let G be a graph with 2n vertices. If for each pair of nonadjacent vertices u and v in G,, then G is IM-extendable. 2 Let G be a claw-free graph with 2n vertices. If for each pair of nonadjacent vertices u and v in G,du+dv≥2n+3, then G is IM-extendable.

研究导出匹配可扩图的度和条件，主要结果如下 1若图G有2n个顶点，且对于G中每一对不相邻的顶点u和v，，则G是导出匹配可扩的 2若G是一个有2n个顶点的无爪图，且对于G中每一对不相邻的顶点u和v，du+dv≥2n+3，则G是导出匹配可扩的。

The content of ferulaic acid is least, its range is 0.496～12.566μg·g~(-1), the content in old-growth Chinese fir soil is more than 5μg·g~(-1), and others are less than 5μg·g~(-1). The content of three phenylic acids in 0~20cm depth soil is larger than 20~40cm. Along the ecosystem conversion direction, in 0~20cm and 20~40cm depth soil, the laws of P-hydroxybenzoic acid, ferulic acid and vanillic acid content are complex, does not exist apparent refularity.

其中对羟基苯甲酸含量最大，大小范围为2.233～19.589μg·g~(-1)，其中只有二代杉木人工林的20～40cm层土壤的酚含量小于5，其他的都大于5；香草酸次之，大小范围为0.912～5.683μg·g~(-1)，其中只有天然更新常绿阔叶林的酚含量大于5，其他的都小于5；阿魏酸含量最小，大小范围为0.496～12.566μg·g~(-1)，其中老龄杉木林的0～20cm和20～40cm层土壤的酚含量大于5，其他的都小于5。

Objective To investigate the effects of interleukin-1 β converting enzyme gene on the biologic characteristics of ovarian cancer cells.

目的 探讨白细胞介素-1 β转换酶的表达，对卵巢癌细胞生物学特性的影响。

Campylobacter: This illness is the most commonly identified cause of diarrheal illness in the world.

弯曲：这种病是最常见的原因查明腹泻病，在世界上。