查询词典 3D

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

The transition dipole moments of neutral and charged Green1 are compared using 3D transition density, which reveal the orientation and strength of transition dipole moments. The charge redistribution of neutral and charged Green1 upon excitation are shown and compared with 3D charge difference density. The electron-hole coherences of neutral and charged Green1 upon excitation are investigated with 2D site representation.

用3D跃迁密度方法对中性和带电的Green1的跃迁偶极矩进行比较，可显示出跃迁偶极矩的取向和强度；用3D电荷差异密度方法显示出激发后的中性和带电的Green1电荷重新分布，也进行了比较：用2D实空间分析方法来研究中性和带电的Green1处于激发态时的电子空穴相干性。

Inition have well-established relationships with the most advanced 3D hardware manufacturers around the globe. From stereoscopic viewing to immersive virtual reality systems, motion capture to 3D scanners and digitisers.

Inition与全球最先进的3D设备制造商建立有紧密的联系，涉及立体视觉、沉浸式虚拟现实系统、动作捕捉、以及3D扫描仪和数字化设备等领域。

InSpeck conducts it own research and development and designs and manufactures non-contact optical digitizers and 3D modeling software.

作为世界闻名的3D技术解决方案供应商，InSpeck是非激光3D光学测量数字化的风向标，主要从事非接触光学数字设备和3D模型软件的自主研发、设计和生产。

The Swift 3D Xpress Scene Editor allows you to make custom changes to your 3D image with our user-friendly trackballs for rotation and lighting, and easy 3D properties dialogues for adjustments to layout, object size, and bevels.

Swift 3D Xpress场景编辑器可以随便你怎么摆弄大小旋转光照倒角哦

The general speedup techniques that concentrate on expediting spatiotemporal coherence from one frame to the next usually uses simplified forms of 3D warp, that is, pixels in current frame are transformed to succeed frames. The approaches of synthesizing images by using optic flow or tensor are under the frame of 3D warp.

McMillan的论文[54]首次详细讨论了单个平面投影图象的3D warp坐标变换公式及其变换过程的兼容可见性扫描算法，本论文在前人工作的基础上，从单个采样图象变换、多个采样图象变换合成、反向变换以及增加镜面反射光等方面深入而系统地研究了3D warp变换进行场景建模和快速绘制的理论和算法。

Based on the geometry constraint and dimensiondriving technique, a 3-UPU parallel simulation mechanism is created. Then a 3D free surface and a guiding plane of tool path are constituted, with the 3-UPU parallel machine tool combined, a novel 3-UPU simulation parallel machine tool for machining a 3D free surface is created.

通过几何约束和尺寸驱动技术，先建立3-UPU并联模拟机构，再构造任意3D自由曲面和刀具轨迹的引导平面，与3-UPU模拟并联机构合成，得到用于加工任意3D自由曲面的模拟并联机床。

Based on the geometry constraint and dimension-driving technique,a 3-UPU parallel simulation mechanism is created.Then a 3D free surface and a guiding plane of tool path are constituted,with the 3-UPU parallel machine tool combined,a novel 3-UPU simulation parallel machine tool for machining a 3D free surface is created.

通过几何约束和尺寸驱动技术，先建立3-UPU并联模拟机构，再构造任意3D自由曲面和刀具轨迹的引导平面，与3-UPU模拟并联机构合成，得到用于加工任意3D自由曲面的模拟并联机床。

At 20.1℃ and RH 48.9%, The longest longevity and mean longevity per fe male and male of south-American leaf miner tested indoor in 1998, the following were 17d, 7d and 5.3d, 3d in 15%cane sugar cott on, and 19d, 6d; 10.6d, 3.5d in broad bean seedling.

在恒温20.1℃，RH48.9%的条件下，用蚕豆苗和用吸足15%的蔗糖水的棉球饲养南美斑潜蝇，测得雌、雄成虫的最长寿命、平均寿命及在蚕豆苗上的最高产卵量、平均产卵量。15%蔗糖水棉球饲养雌、雄成虫最长寿命及平均寿命是17d，7d，5.3d，3d。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。