限制性地

基本解释 (translations)

restrictively

与限制性地相关的网络例句 [注：此内容来源于网络，仅供参考]

Authorize full or limited participation in proceedings before the court by interested persons or agencies, including appearance amicus curiae, intervention as a party pursuant to the Federal Rules of Civil Procedure, examination of witnesses or documentary materials, or participation in any other manner and extent which serves the public interest as the court may deem appropriate

授权利益相关的个人、机构全部或限制性地出席诉讼，包括协助法庭解释法律的人出庭，作为联邦民事程序规则规定的一方当事人的干预，文件材料的检查，或由法院认定的以其它方式和内容参加的诉讼。

He added a gnomic proviso: He wished that only those letters "having bearing on my work" be printed .

他言辞精炼地附加了一个限制性条款：希望只出版"与我的作品有关的信"。

Their majestic repose and massiveness, the somewhat angular and flat elegance of their lines, the restraint shown in the treatment of the difficult material—their whole demeanor indicates a beauty of style that to us, who now can cut the hardest stone like cheese and bread, lacks necessity.

它们宏伟的静卧和重量，稍稍的倾斜和平坦的线段的高雅，对处置困难材料的限制性展示，整一个风度显示了在我们面前的风格的漂亮，那些人是现今能象砍奶酪和面包一样地砍硬石块的人，而实际上是没必要的

Polymarase Chain Reaction may be the better method of detecting M.tuberculosis than other methods and lead us to carry out a series of research on this problem. This thesis is composed of five parts:(1)systematic evaluation for the first time of the ability of five defferent PCR methods to detect M. tuberculosis;(2) using the endonuclease and southern blot by a radioactive and a nonradioactive labeled probe, to confirm the specificity of the PCR products and to analyse the sensitivity of detection by southern blot method using digoxigenin-labeled probe;(3) evaluation of acid-fast staining, bacterial culture and PCR for the detection of M. tuberculosis in sputum, pleural and peritoneal effusion samples;(4) The feasibility of PCR for detection of M tuberculosis on formalin-fixed paraffin embedded tissues;(5) 26 cases of sarcoidosis were retrospectively studiedwith PCR for M. tuberculosis and nontuberculosis Mycobacterium and the problems on histopatholoical diagnosis of sarcoidosis are discussed.

论文共分为五个部分，(1)首次系统地比较了五种不同引物序列的PCR检测结核杆菌方法的特异性和敏感性等情况；(2)利用限制性内切酶酶切分析和同位素及非同位素标记DNA探针进行Southern杂交的办法，分析PCR产物的序列与原设计是否相符及杂交检测PCR产物对提高检测结核杆菌敏感性的情况；(3)并对PCR用于快速检测临床标本中的结核杆菌进行了初步评价；(4)探讨了PCR用于检测石蜡包埋组织中结核杆菌的作用；(5)并利用PCR检测结核杆菌和非结核分枝杆菌的方法对26例原病理诊断为结节病的病例进行了PCR检测及组织病理学诊断的探讨。

This paper summarizes some molecular ecological techniques used in the studies on microbial community structure of oil reservoirs in China, including 16S rRNA gene sequence in the Daqing Oilfield and the northwestward oilfields, 16S rDNA of T-RFLP in the Shengli Oilfield and DNA gene track examination technology in Jilin oilfields, and points out some questions and suggestions for future studies.

本文较系统地总结了我国利用分子微生物生态学方法研究油藏微生物群落结构的进展，其中包括大庆油田和西北部油田的16S rRNA基因序列分析，胜利油田16S rDNA的荧光标记末端多形态限制性片断分析技术，以及吉林油田应用的DNA基因跟踪检测技术，并指出了存在问题和研究方向。

Subsequently, the optimized technology was successfully scaled up to 〓 pilot bioreactor scale by using the parameter simulation method. The scale-down strategy was applied to the 100〓 fermentation tank to ascertain that the determining factor which affects metabolic flux distribution reasonably had changed to the dissolved oxygen control. After resolution of the DO limitation, the optimized technology inhibited the shift on 100〓 production scale correspondingly, notable commercial returns was gained as a result.

随后基于过程参数拟合的方法成功地将抑制代谢流迁移的新工艺放大到12〓规模，并通过规模缩小证实了100 〓生产规模的发酵过程中限制代谢流合理分配的限制性因素已经发生了变化，此时DO的控制成为优化的前提，通过改善供氧条件之后优化的工艺在生产规模也成功的克服了代谢流的迁移，取得了显著的经济效益。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

Congratulations on choosing the dbx 166XL Compressor / Gate. The 166XL provides two channels of noise gating, OverEasy? or classic hard knee compression and PeakStop? limiting to give complete control of signal dynamics to studios, sound reinforcement companies, musicians, or anyone who needs quality processing quickly and easily.

感谢你选购dbx 166XL 压限/噪声器。dbx 166XL 提供两个噪声门通道，OVEREASY或专业硬拐点压缩和峰值终止限制，限制性地完全控制信号提供给录音室，专业音响扩声公司，音乐人，或者任何需要高质量地快速且容易地处理信号的人。

In Chapter 2, the introduction of the dynamical models is presented, furthermore, on the basis of analyzing perturbation factors there are two dynamical models fit for the motion situation of the NEAs, which are imported as the restricted authentic particle model and the restricted mean particle model (with respect to the treatment of the orbits of the perturbing major planets) that the Post-Newtonian effect is also taken into account, while for the latter the influence of the 5/2 (or 2/5) resonant items of the interaction between the Jupiter and the Saturn is considered.

第二章是关于力学模型的介绍，在分析摄动因素的基础上，引入了近地小行星运动中比较合适两种力学模型，即限制性质点真实模型与限制性质点平均模型(这一平均指的是对摄动源大行星轨道的处理)，在两种模型中均考虑了后牛顿效应，另外在平均模型中还考虑了木星与土星之间的5/2(2/5)共振项的影响。

与限制性地相关的网络解释 [注：此内容来源于网络，仅供参考]

conditionally：附有条件地 (副)

conditionality 受限制性; 制约性 (名) | conditionally 附有条件地 (副) | conditioned reflex 条件反射

freehold estate：自主持有地产权

与之相对应则称为自主持有地产权(freehold estate)和租赁持有地产权(leasehold estate). 除了地产权外,英美法也存在地役权(easements)、许可(licences)、获益权(profits a prendre)和限制性契约(restrictive covenants)等非地产权利益.

impede：妨碍

服务自由原则不仅旨在取消那些以国籍为基础的歧视性限制措施,而且还要求取消那些针对本国服务提供者和其他成员国服务提供者非歧视地实施的任何限制性措施,只要后者可能阻止(prohibit)或者妨碍(impede)在另一成员国依法开

our：耗氧速率

杂交瘤细胞的限制性基质补料培养中遇到的一个问题是如何方便,精细地控制关键性底物的浓度,该文在一个10L的生物反应器中,研究了杂交瘤细胞C50在因清培养条件下生长和能量代谢的特性,结果表明,细胞能量代谢和生长密切相关,耗氧速率(OUR)能够及时,敏感地反

prohibit：阻止

欧洲法院开创性地指出,服务自由原则"不仅旨在取消那些以国籍为基础的歧视性限制措施,而且还要求取消那些针对本国服务提供者和其他成员国服务提供者非歧视地实施的任何限制性措施,只要后者可能阻止(prohibit)或者妨碍(impe

Restrictive：限制的

restrictionist 限制主义 | restrictive 限制的 | restrictively 限制性地

restrictively：限制性地

restrictive 限制的 | restrictively 限制性地 | restrike 复击

restrictively：限制性地 (副)

restrictive trade law 限制性贸易法律, (经济用语) 监督限制性安排和专卖业的法律 | restrictively 限制性地 (副) | restroom 厕所 (名)

restrike：复击

restrictively 限制性地 | restrike 复击 | restring 重新装弦

Southern blotting：印迹法

支持物进行的杂交英国的分子生物学家E.M.Southern所发明的Southern印迹法(southern blotting)就是将凝胶上的DNA片段转移到硝酸纤维素膜上后再进行杂交的这里以DNA-DNA杂交为例较详细地介绍Southern印迹法将DNA样品经限制性内切酶降解后,