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This is due to the fact that AFP in the sample flows through the sample pad and binds the conjugate to form a complex. This complex then migrates down to the membrane and binds to the immobilized anti-AFP antibody A2 of the test line, resulting in the immobilization of the reddish conjugate.
如果样品中含有甲胎蛋白，膜上便会出现肉眼可见的微红色检测线，这是由于样品中的AFP与标记有单克隆抗体A1的免疫金结合形成复合物，复合物向膜上移动与固定在膜上的检测线单克隆抗体A2结合反应，结果在该处形成固定的微红色线条。
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To study the surface treatment of the quartz glass, and the coupling of the albumin protein, we used with the fictionalization of the copolymers, and experimental conditions for protein immobilization.
蛋白质固定化之制作程序系将白蛋白固定於涂有光阻剂共聚物之石英玻璃表面，然后检测蛋白质於280 nm波长附近的吸光特性，以评估蛋白质固定化的效果。
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Pike protein consists of S1 and S2 subunit. S1 is head and S2 is rod-shape part, intermolecular effort can bind each other. S1 can bind with host cell epimembranal acceptor. S2 can fix integral Spike protein on the cellular membrane and mediate cell-fusion with virus and host cell. There are significant antigen determinant of virus in Spike protein, genic heterogenesis can effect viral pathogenicity. SARS-CoV Spike protein, which has 23 pieces larvaceous glycosylation sit, consists of 1256 amino acid. Its N-terminals and C-terminals is corresponding conservative.
蛋白是I型膜糖蛋白，介导病毒与宿主细胞膜上的受体结合并与宿主细胞膜相融合。S蛋白由S1和S2两个亚基组成。S1形成球状部分，S2形成棒状部分，两者之间通过分子间的作用力相互结合。S1能与宿主细胞上的受体结合，S2可把整个S蛋白固定在细胞膜上，并介导病毒包膜与宿主细胞膜融合。S蛋白上有重要的病毒抗原决定簇，S基因的突变会影响病毒的致病性。
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Both methods combined the magnetic microspheres technology with hydrazide chemistry and Lectin, respectively. The hydrazide-functionized magnetic microspheres and GNA-functionized magnetic microspheres are prepared correspondingly. The condition for capturing glycopeptides by hydrazide-functionized magnetic microspheres and immobiling GNA onto the magnetic microspheres was evaluated and optimized, and the specificity and validity of two methods was demonstrated by using standard glycoproteins sample.
试验中分别将肼化学和凝集素与磁性微粒相结合，分别制备出了肼功能化磁粒和GNA功能化磁粒，优化了肼功能化磁粒富集糖肽的条件和GNA固定的条件，并通过标准蛋白样品对两种方法的富集特异性和有效性进行了验证。
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The S 2p, C 1s, N 1s and O 1s spectra were typically analyzed. Experimental result has demonstrated that using MUA/Au, Ag as the substrates, collagen immobilization process in wet cell system or in atmospheric environment did not have qualitative or quantitative difference. Firstly, our well cell system is feasible to carry out on-line collagen immobilization process.
实验结果显示：具有羧基之 MUA /Au，Ag 於真空湿室反应室中及传统大气环境下固定胶原蛋白，试片在两种环境所固定的胶原蛋白结构，在定性及定量分析上无显著差异，先证实在本真空湿室反应室中进行连续的蛋白质固定程序具体可行。
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The result of immobilization showed that hydrophile membranes were more advantageous than hydrophobe ones to act as immobilization carrier. Crosslinking organophosphorus hydrolase by glutaraldehyde on polyethersulfone membrane is a simple and practical immobilization method. An appropriate amount of bovine serum albumin and crosslinker is necessary to get a good result of immobilization. In the biodegradation of methyl-parathion through enzyme membrane reactor, the effect of immobilized enzyme amount, flow rate of peristaltic pump, pH of the feed, and the methyl-parathion concentration on biodegradation rate was studied. It was shown that the biodegradation rate increased with immobilized enzyme amount and substrate concentration. Biodegradation rate didn't increase when the folw rate of the peristaltic pump was larger than 7 ml/min.
然后，研究固定化酶降解甲基对硫磷，从不同材料的膜载体固定有机磷水解酶的比较中，发现亲水膜比疏水膜适合作固定化酶载体；在此基础上，将有机磷水解酶固定在聚醚砜微滤膜上，并制成酶膜生物反应器，用于降解甲基对硫磷；戊二醛化学交联法固定时，酶液与10%牛血清白蛋白溶液的比例在3：1~2：1，与交联剂的比例在7：2~7：4范围内，固定化效果较好，并发现牛血清白蛋白和膜载体有降低酶活损失的作用；将固定化的酶膜装于酶膜反应器降解甲基对硫磷，实验结果表明：反应器的降解速率与固定化酶量呈正比，也随底物浓度的增加而加快，当流速低时，降解速率随流速增大而加快，到7ml/min以上时，降解速率不随流速脑黾佣洌到獾淖畹团ǘ任？
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Acceptable resolutions were achieved when it was applied for the separation of dipeptides including Leu-Tyr and Val-Tyr by using 1-butanol-acetic acid-water (4:1:5, V/V/V) solvent system. The proteins including cytochrome C and myoglobin, lysozyme and myoglobin, and fresh chicken egg-white proteins were well separated by 12.5% PEG1000-12.5% K2HPO4-75% water (pH 9.0) and 16% PEG 1000-12.5% K2HPO4-71.5% water (pH 8.0) system.
应用研究表明，采用正丁醇-醋酸-水(4：1：5， V/V/V)和PEG1000-磷酸钾盐-水(12.5：12.5：75， W/W/W)(pH 9.0)体系可以在较高的流动相流速和较高的固定相保留下分别实现对亮氨酸-酪氨酸和缬氨酸-酪氨酸二肽混合物以及细胞色素C与肌红蛋白、肌红蛋白与溶菌酶混合物及鸡蛋清样品中蛋白成分的成功分离。
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Preliminary studies on affinity isolation based on the specific combination between Biotin and Avidin Presently, affinity chromatography is increasing to be a main protein isolation and purification technique for a complicated system because of its high specificity, high rate of recovery, etc.
利用生物素和抗生物素蛋白间的特异性结合作用，将生物素通过化学反应键合到蛋白质的某种官能团上，酶解后的肽段通过固定了抗生物素白蛋白的亲和分离凝胶色谱柱，可以选择性地分离生物素化的肽段，达到简化复杂体系的目的。
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II. Exploring non-radioactivc method to select the optimal DNA-bindingsequencc fOr singIe-chain reprcssorCloning and expression of single-chain repressors with cysteine tai1 wereperformed and immobilized these protein in the solid surface by using the standardmicrowell plates Which have been activated to contain maleimide grouPs, al1owing tobind sulthydryl-containing protein on its surface.
二。非同位素的方法筛选单链阻遏蛋白的最佳DNA结合序列初探克隆和表达了带半眈氨酸尾的单链阻遏蛋白，利用己包彼了马来酚胺的活性板可以与自由琉基结合的特性，将蛋白固定在活性板表面。
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The expressed E2 protein was conjugated to ELISA microplate which was precoated with anti-E2 mAb H53. Then Raji cells were seeded onto the microplate and cultured .
将表达的E2蛋白固定在事先包被了E2单抗的酶标微孔板上，然后加入Burkitt淋巴瘤Raji细胞，可观察到该细胞聚集贴附生长。
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acetic anhydride：乙酸酐
这种广泛的去蛋白作用无疑可增强不少实验工作者在多聚甲醛固定后,浸入乙酸酐(acetic anhydride)和三乙醇胺一个重要的问题. ISHH 中背景染色的形成是诸多因素构成的. 杂交后(Posthybridization )测系统检测组织细胞中的抗原时,
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central element：中央成分
联会复合体:是同源染色体联会过程中形成的非永久性的复合结构,主要成分是碱性蛋白及酸性蛋白,由中央成分(central element)向两侧伸出横丝,使同源染色体固定在一起.
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RIE：火箭免疫电泳
免疫电泳技术的种类很多,如:对流免疫电泳(CIEP)、火箭免疫电泳(RIE)、电免疫扩散(EID). 在各种免疫电泳方法基础上,又不断地派生出一些新的技术,如:免疫电泳(IEP)技术,1969年Alper与Johnson推荐免疫固定电泳(IFE)是一种包括琼脂糖凝胶蛋白电泳和免疫沉淀两个过程的操作,
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Tannic acid：丹宁酸
+- **丹宁酸(Tannic acid)是一种从植物中提取的物质,泛指五倍子酸(gallic acid)糖苷或多聚糖苷. 大分子很难渗透到组织中,因此电镜中使用低分子量的丹宁酸. 丹宁酸既是一种强的固定剂,能固定许多蛋白以及糖类衍生物,又是一种媒染剂,
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synaptic vesicles：突触小泡
2008年,Hell小组使用STED技术通过抗体标记目标蛋白,观察到了活体神经元细胞中突触小泡(synaptic vesicles)的运动过程. 同年稍晚些时候,他们又使用4Pi显微镜和STED技术得到了固定细胞内线粒体的3D图像,分辨率达到了40至50nm. 最近,
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lipophilic：亲脂性的
lipopexia 脂肪固定 | lipophilic 亲脂性的 | lipoprotein 脂蛋白