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Change in the concentration of endogenous IAA was measured with enzyme-linked immunosorbent assay, and the distribution of IAA-binding protein in the protoplasts in the cambial region was tested with an immuno-fluorescence technique, being improved by the authors, during the cambial active period in Broussonetia papyrifera L.
构树(Broussonetia papyriferaVent。)形成层活动周期中用酶联免疫吸附法测定了形成层区域的内源IAA浓度的变化,并用自行发展的免疫荧光检测方法测定了由此处生活细胞制得的原生质体中IAA结合蛋白的分布。