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Objective To investigate the characteristics and application of extra cellular matrix of conjunctiva.
目的 观察结膜细胞外基质(extracellularmatrix ，ECM)作为结膜组织的替代材料用于结膜成形的特点及临床疗效。
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There had no marked change in animals treated with E2 at dose of 20 mgkg^(-1)d^(-1). CONCLUSION E2 at dose of 200 mgkg^(-1d^(-1) has marked therapeutical effect on KCS by inhibiting T lymphcell, increasing the amount of goblet cell and AQP3 in conjunctiva.
较大剂量的雌激素对结膜干燥综合征有明显的治疗作用，治疗的机制包括增加结膜杯状细胞的数量，上调结膜上皮细胞、杯状细胞及腺样层AQP3的表达量，抑制林巴细胞；但普通剂量的雌激素无明显治疗作用。
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Results一. Culturing and identification of human conjunctiva cells1. morphous of conjunctiva cellsCells were harvested by tissue cultivation after digestion. Ponderosus cells adhered after 48 hours. Cells were shown in shape of round, ellipse and polygon. Cell body was loose and lucency with nucleus in center, cell membrane was also clearly seen. Cells were arranged in inlay and fuse in film after 12-14 days.2. Immunochemistry testNomogeneous immunochernical positive granules against CK13 were observed in endochylema of human conjunctiva cells.3. growth curve of conjunctival cellsSubcultured cells were aged and feeble after 5th passage.
结果一、正常人结膜细胞的培养与鉴定1、活体细胞的形态消化后组织块培养法：48小时后见大量上皮细胞贴壁，细胞不规则呈圆形、椭圆形、多边形，细胞体肥大透亮，细胞核位于中央，胞膜清楚，约12-14天后细胞呈镶嵌状排列，融合成膜状。2、免疫组化鉴定培养的人结膜上皮细胞CK13免疫组化染色见阳性颗粒均匀分布于胞浆中。3、培养结膜细胞生长曲线传代培养多数样本于P5代以后开始出现衰老征象。
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In the other group, muscle slippage occurred in 2 cases, bulbar conjunctiva hyperemia, subconjunctival hemorrhage and edema occurred in 40 cases, conjunctival scar occurred in 10 cases, conjunctiva cyst 2 cases and conjunctival polypus 3 cases.
直视下斜视矫正术组：术后发生肌肉滑脱的2例，球结膜伤口充血、结膜下出血、水肿者40例，结膜瘢痕10例，结膜囊肿2例，结膜息肉3例。
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objectiveto explore the drain way to tear with inferior lacrimal passage ruined critically.methodsthe special silicon tube whose bore 1mm and diameter 2mm was inserted between conjunctival sac and nasal vestibule,the tube was placed under the soft tissue of bucco-nasal groove and in the surface of the protrusion of upper jaw bone.resultsthere were 32 patients who undergone tube above five years.one patient of them was pulled out the tube because of papilloma hyperplasia,the other patient was pulled out the tube with the tube slide below,and the rest 30 patients kept the drain well and co
目的探讨下泪道破坏严重时泪液引流途径。方法在结膜囊-鼻前庭间安置一个内径1mm、外径2mm的特制硅胶导管，此管置于鼻颊沟软组织下，上颌骨前突表面。结果治疗32例，观察5年以上，其中1例因结膜乳头状瘤增生而拔管，1例因导管下滑而拔管，30例引流良好，病人亦能耐受。本文就引流机制、并发症、机体耐受问题和操作进行了详细地讨论。结论泪道外插管是下泪道严重损害时泪液引流的一种较好的解决方法。皮下；结膜鼻腔造瘘术；硅胶导管
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It is an ideal conjunctival succedaneum for conjunctiva in conjunctiva transplantation and could be used as a graft to reconstruct ocular surface as well.
结膜ECM为目前比较理想的结膜替代材料，并可有效地用于角膜、结膜表面重建。
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The objective of this study is to investigate the localization of the conjunctival stem cells in adults.
进一步研究结膜干细胞的确切定位及生物学特性、体外分离培养结膜干细胞、用组织工程构建生物结膜是结膜干细胞的研究方向。
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The results are unsure for experimental use;The rabbit's corneas that were removed with upper-half of corneal limbal epithelium lamella and erased the center corneal epitheliums were transparent with intact corneal epithelium;In the approach,the corneal and limbal epitheliums were burned with a cotton swab socked in 1 mol/L NaOH,there were 4 rabbits' corneal stroma happened perforation or ulcer and symblepharon,and the other one presented corneal epithelium phenotype.This is an applicable method to create the pathological model of corneal limbal stem cell total deficiency.
结果表明，处理后4周，全周角膜缘上皮板层手术切除，中央角膜上皮层用1 mol/L NaOH擦除的5只试验家兔角膜表面全部血管化、结膜化，未发生睑球粘连，角膜基质胶原纤维完整未见溃疡、穿孔等病变，细胞印迹学检查为结膜表型，可作为实验性角膜缘干细胞移植的病理模型；全周角膜缘上皮板层手术切除，中央角膜上皮用生理盐水擦除的5只试验家兔，有2只为结膜表型，另3只为角膜表型，观察期内结果不稳定；半周角膜缘上皮板层手术切除，中央角膜上皮层用生理盐水擦除的5只试验家兔，角膜表面透明，全部为角膜表型；直接用1 mol/L NaOH擦除角膜缘和中央角膜上皮的试验家兔，有4只角膜基质胶原纤维断裂、溶解，并伴有严重的溃疡、穿孔、睑球粘连等病变，不能用于移植试验，另1只角膜表面透明，未见结膜和新生血管长入，细胞印迹学检查为角膜表型。
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Immunohistochemical identification of cultured conjunctival cellsWhen the second cultured generation of conjunctival cells close to confluence, the glass coverslips were removed in situ fixation by methanol and DAB color immunohistochemical staining by CK13 monoclonal antibody in time.3. growth curve of conjunctival cellsThe 1st,3rd,5th passage cells were selectecd randomly and subcultured at the density of 5×10~4/ml,after culturing for another 1-9 days, were counted and compared the cell number every day.
采用消化后组织块法培养结膜原代细胞，并进行传代。2、培养结膜细胞的免疫组化鉴定把第2代的结膜细胞接种于置在培养皿中的盖玻片上，当细胞接近融合时，及时取出作原位甲醇固定，CK13单抗、DAB显色免疫组化染色。3、结膜细胞生长曲线的测定随机选取第1、3、5传代细胞，以5×10~4／ml密度传代、分别培养1-9天，每天分别细胞计数并进行分析比较，重复3次。
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6Experiment with rose Bengal staining: glass rod was used to get 10 g/L rose Bengal and drop in conjunctival sac. After eyes blinking for several minutes, the observation was performed with green light filter under slit lamp (evaluation criteria: corneal conjunctiva of palpebral fissure stained+, stained to the inferior bulbar conjunctiva++,stained to the superior bulbar conjunctiva). Dry eye disease was diagnosed indirectly with red-stained epithelial cells and mucin.
6孟加拉玫瑰红染色试验：用玻璃棒沾取10 g/L孟加拉玫瑰红染料滴入结膜囊瞬目数分钟之后，在裂隙灯下用无赤光观察(评分标准为以睑裂部角结膜染色为+，染色累及下方球结膜为++，累及上方球结膜为+++)，以其染红上皮细胞和黏蛋白间接诊断干眼病。
- 更多网络解释与结膜相关的网络解释 [注：此内容来源于网络，仅供参考]
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conjunctiva：结膜
睑结膜: 结膜(conjunctiva)为连结眼睑和眼球的透明薄膜,含有丰富的血管和神经末梢. 衬在眼睑内面的称睑结膜.
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palpebral conjunctiva：睑结膜
1.睑结膜(palpebral conjunctiva)紧密附著於眼睑后面,连接眼睑内面到眼睑缘,与睑板紧密连接,不能移动. 2.穹窿结膜(fornical conjunctiva)是睑结膜和球结膜的移行部,爲结膜最松弛的部分. 3.球结膜(bulbar conjunctiva)覆盖在眼球前部,巩膜外面.
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bulbar conjunctiva：球结膜
2.球结膜(bulbar conjunctiva) 覆盖于眼球前部巩膜表面,止于角巩膜缘,是结膜的最薄和最透明部分,可被推动. 球结膜与巩膜间有眼球筋膜疏松相连,在角膜缘附近3mm以内与球筋膜、巩膜融合. 在泪阜的颞侧有一半月形球结膜皱褶称半月皱襞,
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bulbar conjunctiva：球结膜;眼球结膜
\\"延脑中枢\\",\\"bulbar center\\" | \\"球结膜;眼球结膜\\",\\"bulbar conjunctiva\\" | \\"球海绵肌\\",\\"bulbo-cavernosus muscle\\"
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conjunctiva concretion：结膜结石
subconjunctival hemorrhage 结膜下出血 | conjunctiva concretion 结膜结石 | conjunctiva angioma 结膜血管瘤
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Fornix of：穹窿部结膜
folliculosis 结膜滤泡增生症 | Fornix of 穹窿部结膜 | Hyperemia of 结膜充血
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conjunctival fornix：结膜穹隆
结膜 conjunctiva | 结膜穹隆 conjunctival fornix | 结膜囊 conjunctival sac
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xerosis of conjunctiva：结膜干燥症
结膜干燥症(xerosis of conjunctiva)主要由于结膜组织本身的病变而引起的结膜干燥现象. 在正常情况下,泪腺和结膜杯状细胞分泌的泪液使结膜经常保持湿润,一旦上述的滋润机能遭到破坏,即出现结膜干燥症.
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conjunctival folliculosis：结膜滤泡症
1.结膜滤泡症(conjunctival folliculosis)常见于儿童,皆为双侧,无自觉症状. 滤泡多见于下穹隆部与下睑结膜. 滤泡较小,大小均匀相似,半透明,境界清楚,滤泡之间的结膜正常,不充血,无角膜血管翳,无瘢痕发生. 沙眼的滤泡多见于上穹隆部与上睑结膜,
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Fornical conjunctiva：穹窿结膜
2.穹窿结膜(fornical conjunctiva)是睑结膜和球结膜的移行部,爲结膜最松弛的部分. 3.球结膜(bulbar conjunctiva)覆盖在眼球前部,巩膜外面. 3.结膜腺体(1)杯状细胞(goblet cells) 位於结膜上皮细胞层,多见於球结膜区域,在角巩缘和睑缘部附近缺如.