片段

基本解释 (translations)

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与片段相关的网络例句 [注：此内容来源于网络，仅供参考]

Appressoria were latent in intercellular cleft and were latent until banana fruit were harvested.The development process of conidia of Colletotrichum musae on fruit was not distinct from foliage and stalk.Two pairs of PCR primers were designed according to the two especial fragment (357bp and 206bp) of Colletotrichum musae. Banana tissue culture seedling genomic DNA, banana anthracnose pathogen genomic DNA, mango anthracnose pathogen genomic DNA, rubber anthracnose pathogen genomic DNA,watermelon anthracnose pathogen genomic DNA, banana crown rot pathogen genomic DNA, stylo anthracnose pathogen genomic DNA, watermelon Fusarium wilt pathogen genomic DNA were extracted using SDS method.

根据香蕉炭疽菌的两个特异片段(分别为357bp和206bp)，设计两对引物：采用SDS法分别提取了香蕉组培苗基因组DNA、香蕉果实炭疽菌弱致病株Z_1基因组DNA、香蕉果实炭疽菌强致病株Z_4基因组DNA、芒果炭疽菌基因组DNA、橡胶炭疽菌基因组DNA、柱花草炭疽菌基因组DNA、西瓜炭疽菌基因组DNA、香蕉冠腐病菌基因组DNA、西瓜枯萎病菌基因组DNA；以上述基因组DNA为模板对特异片段进行PCR验证，证明357bp的片段为Colletotrichum musaes所特有，可以用此片段进行香蕉果实炭疽病的分子检测试验，。

It was not cross- reaction with Entamoeba histolytica and Schistosoma japonica.It is a simply and better method to extract the DNA with lysine boiling.

结果 经扩增于500bp、670bp、1200bp出现特异性片段，与原选择的编码基因片段大小相同，此特异片段与溶组织内阿米巴、日本血吸虫DNA无交叉反应，不同的DNA提取法结果显示：用裂解煮沸法同样可从仅含1条旋毛虫幼虫的25μl正常小鼠血浆中扩增出特异性片段。

These specific cDNAs and DNAwere subcloned into vector for sequencing.The sequencing results demonstrated thatthe specific cDNA from P.chinensis eyestalk consists of 203 base pairs,and all thespecific cDNAs from the shrimps T.curvirostris,P.stylirostris and the crab E.sinensis consist of 215 base pairs.

分别将这些片段亚克隆到载体中进行测序，测得中国对虾特异性cDNA片段由203个碱基组成，中华绒螯蟹、鹰爪虾、蓝对虾的特异性cDNA片段及蓝对虾的特异性DNA片段由215个碱基组成，其中蓝对虾的特异性cDNA与由蓝对虾的基因组DNA扩增得到的特异性DNA片段的碱基序列几乎完全相同。

The result showed that twocDNA fragments which expressed at high level both in shoot and radicle representedthe gene encoding beta-D-glucosidase; one cDNA fragment expressed specifically inshoot represented the gene encoding mitochondria HSP60; most clones of MF12 andMF17 fragments respectively represented the chloroplast genes encoding prp22 andprp19 proteins which are two components of ribosomal small subunit; while thededuced amino acid sequence from each exceptional one clone was respectivelyhomologous to CDC5 proteins and vesicle-associated membrane proteins; otherthree cDNA fragments expressed preferentially in shoot had no homologue inGenBank.

结果发现2个在胚芽和胚根中表达量都很高的cDNA片段代表的是编码玉米β-D-葡糖苷酶的基因；一个在胚芽中表达而在胚根中不表达的片段代表的是编码线粒体分子伴侣HSP60的基因；片段MFl2的大部分克隆测序结果是与叶绿体基因组中编码核糖体小亚基蛋白prp22的基因同源，但其中有一个克隆测定的cDNA片段序列推测的氨基酸序列与CDC5家族成员有较高的同源性；片段MF17的大部分克隆测序结果与叶绿体基因组中编码核糖体蛋白prp19的基因同源，而有一克隆测定的cDNA片段序列推测的氨基酸序列与参与信号转导的膜结合蛋白VAP27和VAMP有较高的同源性；另有3个优先在胚芽中表达的cDNA片段未查询到同源序列。

The results showed that the PCR fragment length of root-knot samples was 768bp in cowpea(RKN-1), tomato(RKN-2), balsam pear(RKN-3), Astragalus adsurgens (RKN-19)and rose(RKN-4) from Beijing, peanut fromWeifang (RKN-7) Yantai(RKN-6) and Jiaozhou(RKN-5) in Shandong, tomato from Hexian(RKN-9), cucumber from Suzhou (RKN-8) and Platycodon grandiflorum from Taihe(RKN-11) in Anhui and two kinds of tomato species(RKN-22,23) from China Central Agricultural University; the PCR fragment length of root-knot samples was 769bp in balsam pear from Ganyu(RKN-10) in Jiangsu, tomato from Kunming(RKN-12) in Yunnan, Langfang (RKN-17)in Hebei, Chengdu(RKN-18) in Sichuan and Suzhou(RKN-21) in Anhui and cucumber from Sixian(RKN-20) in Anhui; the PCR fragment length of root-knot samples was 772bp in guava(RKN-13) and pawpaw (RKN-15) from Hainan; the PCR fragment length of the other two samples was both 766bp in tomato(RKN-24,25) from China Central Agricultural University, and the PCR fragment length of root-knot nematodes in cucumber from Hangzhou in Zhejiang(RKN-16) and pepper from Anding (RKN-14)in Hainan was 767bp and 869bp, respectively.

结果表明，北京密云的豇豆、番茄、苦瓜，北京植保站月季，山东胶州，烟台和潍坊的花生，安徽和县的番茄，宿州的黄瓜和太和的桔梗，北京畜牧所沙打旺以及中国农业大学两个番茄品种(RKN-23和RKN-25)上的根结线虫种群的PCR扩增片段长度为768bp；江苏赣榆苦瓜，云南昆明，河北廊坊，安徽宿州和四川成都番茄以及安徽泅县黄瓜上根结线虫种群的PCR扩增片段长度为769bp；海南安定木瓜和石榴上根结线虫种群的PCR扩增长片段度为772bp；中国农业大学另外两个番茄(RKN22和RKN-24)品种上的根结线虫种群的PCR扩增片段长度为766bp；而浙江杭州黄瓜和海南安定胡椒上的根结线虫种群的PCR扩增长度分别为767bp和869bp。

Results (1) The forward and reverse subtracted cDNA libraries of different metastastic potential large cell lung cancer cell lines were successfully constructed;(2) With blue-white colony and dot blot, 307 positive clones in the forward subtracted library and 78 positive clones in the reverse subtracted library were obtained;(3) 55 clones were successfully sequenced in the forward subtracted library. Homolog analysis confirmed 23 differential expression segments, which were similar to the human genes already known, including NME2, NPM1, MT 2A, HSPE1, TAFIA, EPRS, PX19 and EIF3S9 et al;(4) 31 clones were successfully sequenced in the reverse subtracted library. Homolog analysis confirmed 16 differentially expressed segments. 15 of them were similar to the human genes already known, including ANXA2, TUBB, PKN2, GNAS, EEF1A1, SSR2 and RPLPO et al. Only one segment had partial homology to known human genes. This segment was supposed to be the new EST segments which might not have been cloned.

结果(1)成功构建人大细胞肺癌高低转移株差异表达基因正向消减cDNA文库和反向消减cDNA文库；(2)经蓝白菌落筛选和斑点杂交，正向消减文库获得307个阳性克隆，反向消减文库获得78个阳性克隆；(3)正向消减文库挑选55个克隆成功测序，经同源性分析最终确定差异表达基因片段23个与已知人类全长基因具有很高相似性(95％～100％)，这些基因包括NME2、NPM1、MT2A、HSPE1、TAFlA、EPRS、PX19和EIF3S9等；(4)反向消减文库挑选31个克隆成功测序，经同源性检索和比对最终确定差异表达基因片段16个，其中15个与人类全长基因具有很高相似性(95％～100％)，包括ANXA2、TUBB、PKN2、GNAS、EEF1A1、SSR2和RPLPO等基因。1个片段和己知人类基因仅有部分相似性，表明它可能是未被克隆的人类基因EST片段。

Sequencing analysis and database searches indicated that there were 19 known genes and 31 unknown cDNA fragments in the sequenced 72 dot blot positive clones specific for gastrula embryos,and 52 known genes and 37 unknown cDNA fragments in the sequenced 98 dot blot positive clones specific for tail bud embryos.

测序和基因数据库比对结果表明，72个原肠期斑点杂交阳性克隆中，包括19个已知基因的cDNA片段和31个没有同源性的cDNA片段；98个尾芽期斑点杂交阳性克隆中，包括52个已知基因的cDNA片段和37个没有同源性的cDNA片段。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Total RNAs from KAx-3 cells and AK127 cells(developed for 14h) were isolated. After the reverse transcription and PCR reaction, two distinct differential fragments were acquired., fragment A was from KAx-3 cells and fragment C was from AK127 cells. After retriving and reamplifying the differentially expressed fragments, white-blue plaqueselection, the fragments were purified. Northern blot proved that fragment A was from KAx-3 cells and fragment C was from AK127 cells. The results of sequencing and researching for NCBI database have been showed: part sequence of fragment A shows 91% similarity to the gene encoding DhkA, 92% similarity to the gene encoding DhkF, 91% similarity to the gene encoding STATc, 97% similarity to the homoeobox gene encoding protein. These genes play important part in controlling cell differentiation and cell proportion in Dictyostelium discoideum.

本研究通过提取盘基网柄菌发育14小时的野生型KAx-3细胞和突变型AK127细胞的总RNA，运用mRNA差异显示法分离出了两条明显的差异表达片段，其中片段A来自野生型KAX-3细胞，片段C来自突变型Ak127细胞；并通过凝胶回收差异片段、对差异片段进行再次PCR、蓝白斑筛选克隆、提取质粒、酶切电泳纯化差异片段；接着进行Northern杂交的结果表明，片段A只与野生型KAx-3细胞的总RNA有杂交信号，片段C只与突变型AK127细胞的总RNA有杂交信号，这就排除了差异片段假阳性的可能；最后通过测序，搜索NCBI BLAST数据库发现：片段A的小部分序列与编码组氨酸激酶DhkA基因中一段序列的相似性高达91%，与编码组氨酸激酶DhkF基因中的一段序列相似性高达92%，与编码STATc蛋白基因的一段序列相似性达91%，以及与编码同源框蛋白的基因中的一段序列相似性达97%，这些基因在盘基网柄菌细胞分化和细胞比例调控过程中起着相当重要的作用，这些数据进一步说明了突变细胞不能完成发育的原因。

Five pairs of primers were designed for PCR according to five fragments of the putative virulent genes acquired by suppression subtractive hybridization between virulent strain HA9801 and avirulent strain 12 of Streptococcus suis type 2 （ SS2 ）. The five fragments were transcriptional regulator (15# and 154# fragments), amino acid permease (210# fragments), ABC transporter (9# fragments) and surface anchored protein (187# fragments).

通过猪链球菌 2 型（ Streptococcus suis type 2， SS2 ）毒力菌株 HA9801 与无毒力菌株 12#之间的抑制性差减杂交实验，获得了可能与 SS2 菌株毒力相关的 5 个基因片段，分别是转录调节子（ 15#，和 154#片段），氨基酸通透酶（ 210#片段）， ABC 转运子（ 9#片段）及表面锚定蛋白（ 187#片段）。

与片段相关的网络解释 [注：此内容来源于网络，仅供参考]

Clips：片段

近年来片商为了票房在行销上多动头脑,有手法低劣者把电影正片精彩片段通通剪入 预告或者以片段(clips)的方式吸引观众进戏院. 殊不知精彩片段甚至关键剧情已全部出现在预告及片段中,即便电影本身不差,看来也 是索然无味,

Okazaki fragment：冈崎片段

这种小片段被称为冈崎片段(Okazaki fragment). 因此,复制时亲代DNA分子中那股3′→5′方向的母链作为模板,指导新链以5′→3′方向连续合成,此链称为前导链(1eading strand). 在前导链延长1000 ~2000个核苷酸后,

fragmenting：片段化

"片段透通性","fragmentation transparency" | "片段化","fragmenting" | "框,资讯框","frame"

Horizontal Center：将所有选取的片段以最大的片段水平中心为基准对齐

.Left:将所有选取的片段以最大的片段左边为基准对齐. | .Horizontal Center:将所有选取的片段以最大的片段水平中心为基准对齐. | .Right:将所有选取的片段以最大的片段右边为基准对齐.

segments：片段

中文摘要 流感A型病毒的遗传物质为单股负链RNA,由八个片段(segments)组成,第八个片段编码出的蛋白之一为流感A型病毒的非结构蛋白1(Non-Structure protein 1).

Cap Segments：顶部的片段数

Height Segments 高方向上的片段数 | Cap Segments 顶部的片段数 | Sides 边数

Height Segments：高方向上的片段数

Height Segments 高方向上的片段数 | Cap Segments 顶部的片段数 | Sides 边数

time slice,multiplexing：多任务时间片段;多任务时间截分;多任务时间区分

time slice 时间片段;时间截分;时间区分 | time slice, multiplexing 多任务时间片段;多任务时间截分;多任务时间区分 | time slice, statistical multiplex-ing 统计多任务时间片段;统计多任务时间截分;

timeslice,multiplexing：多任务时间片段;多任务时间截分;多任务时间区分

timeslice时间片段;时间截分;时间区分 | timeslice,multiplexing多任务时间片段;多任务时间截分;多任务时间区分 | timeslice,statisticalmultiplex-ing统计多任务时间片段;统计多任务时间截分;

Vertical Center：将所有选取的片段以最大的片段垂直中心为基准对齐

.Top:将所有选取的片段以最大的片段顶端为基准对齐. | .Vertical Center:将所有选取的片段以最大的片段垂直中心为基准对齐. | .Bottom:将选取的片段以最大的片段底部为基准对齐.