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P28 紫色 Formation of a peptide bond 肽键形成–4侧链具有的反应–形成二硫键-其它反应 Tyr,His,Arg Review Isoelectric Point Titration滴定 of an amino acid 滴定 Gly幻灯片 18 His Lys Glu Reaction of amino acids Section 4 Protein isolation and purification 蛋白质分离及提纯 Working With Proteins Experimental techniques for protein analysis and characterization Purification steps A cell contains many types of proteins In the lab we want to isolate a single protein for experiments Purification steps We first grow cells or isolate tissues that contain the protein of interest We break open the cells to produce a crude extract Use centrifugation离心 to separate soluble from insoluble material We fractionate 分离 the protein mixture based on properties of such as size, charge affinity or solubility.
丹磺酰氯与氨基酸反应生成荧光性质强和稳定的磺胺衍生物，用于多肽链NH 用于多肽链 3末端氨基酸的标记烃基化反应(1) 2，4-二硝基氟苯2，4-dinitrofluorobenzene，二硝基氟苯(二硝基氟苯 DNFB也叫做试剂。DNFB在弱碱性溶液也叫做Sanger试剂试剂中与氨基酸发生取代反应，生成黄色化合物二硝基二硝基苯基氨基酸(dinitro phenyl amino acid， DNP氨基酸氨基酸)苯基氨基酸氨基酸(2)苯异硫氰酸酯(phenylisothiocyanate， PITC)在弱碱性条件下，与氨基酸反应在弱碱性条件下，生成苯乙内酰硫脲 PTH衍生物，(phenylthiohydantoin， PTH)衍生物，即PTH-氨基酸，此反应又称之Edman反应，该反应是蛋白质或多肽氨基酸序列测定常用的反应。
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In certain conditions previously inhibited conditioned reactions can ''.
在一定的条件下，先前受到抑制的条件反应''。
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The factors affect on the degree of substitution of product, yield and apparent viscosity, such as the mol rates of EPTAC with -NH2, reaction temperature, reaction time and the content of water in system have been discussed in detail. FTIR spectrometer and UV spectrometer are used to characterize the structure of the derivative and it is proved that substitution reaction mainly occurs on the -N element .The particle size and morphology of chitosan and quantemary ammonium salt of chitosan are analysised by comparing photos of their scanning electron microscope. The degree of substitution of HACC is determined by acid -base conductometric titration. An error in current formula of DS has pointed out and a correct formula has presented.On the base of experience of above experiment, the reaction conditions of HACC were systematically investigated through orthogonal design, and then the best conditions are found. Water-solubility and dependence of pH are studied.
壳聚糖和环氧丙基三甲基氯化铵在异相条件下发生反应，通过单因素实验，制备了一系列不同性能的壳聚糖季铵盐，本文首次详细讨论了反应条件(反应摩尔比、反应时间、反应温度、水含量)对产率及产物性能的影响；用红外光谱和核磁共振对结构进行表征分析时，发现季铵盐阳离子基以N位上取代为主；通过电镜图片对壳聚糖和季铵盐衍生物外观形貌进行对比，采用酸碱电导滴定法测定产物的取代度，并对常用取代度公式做了修正。
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Extracellular single unit recordings in vivo were performed in the DG of learner animals during the consolidation of trace eyeblink CRs.
采用在体单细胞外记录技术，研究习得条件反应豚鼠的齿状回颗粒细胞在条件反应巩固过程中的放电活动。
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The results revealed that all the trace-conditioned animals acquired the trace eyeblink CRs over 14 training days, however, none of the pseudo-conditioned animals did.
结果显示：a。通过14天的训练，条件反射组动物均建立了追踪性眨眼条件反应，而非配对组动物则没有建立该条件反应；b。
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The results suggested that the DG might participate in the neural circuit important for the consolidation of trace eyeblink CRs, and that the granule cells might encode different information during the consolidation of trace eyeblink CRs.
这些结果提示：齿状回可能参与巩固追踪性眨眼条件反应所需的神经环路，其颗粒细胞在追踪性眨眼条件反应巩固过程中可能编码不同的信息。
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On the basis of the above-mentioned results we suggest that the mammillary body exerts an inhibitory effect in the process of conditioned response.
基于上述结果提示，乳头体在条件反应过程中起抑制作用，在建立这种反应的早期能影响情绪、注意和感觉信息的整合。
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High-pressure liquefaction of a sawdust in syngas and hydrogen with tetralin as solvent was performed in an autoclave,and the effects of temperature,reaction time and initial reaction pressure on the sawdust high-pressure liquefaction were studied by testing the distribution and characteristic of liquefaction products.
在小型高压反应釜中以四氢萘为溶剂，氢气和合成气为液化反应气，通过对不同液化条件下所得液化产物的收率及性质分析，考察了不同液化条件(反应温度、反应时间、反应气压力)对锯屑高压液化行为的影响；同时在相同液化条件下，通过液化产物收率和性质的分析，考察了气氛对锯屑高压液化行为的影响，探讨了用合成气代替氢气进行液化的可行性。
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In the same reactor, telon fast green 5G was synthesized through condensation and sulfonation processes with 1,4,5,8-hydroxyanthraquinone and p-toluidine as main raw materials.
由1，4，5，8—四羟基蒽醌和对甲苯胺为主要原料，在一个反应器内同时完成了缩合和磺化两步单元反应，合成酸性蒽醌绿染料，并对其反应条件对产物收率的影响进行了研究，确定了最佳合成工艺条件(反应时间16 h、反应温度120℃、原料的配比1：2.2)。
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Psychology of a conditioned response; caused to die out because of the absence or withdrawal of reinforcement.
指一种条件反应；因为强化作用的消失或者减弱而导致条件反应的渐渐消失。
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Clark：柯拉克
然而,汤森的学生柯拉克(Clark)发现,除掉海马回的兔子依然可以学会古典式的眼睑条件反应. 一些研究指出,兔子可以完全没有视丘(thalamus)以上的结构,而仍可以学会该反应. 由此可见,海马体对於学习眼睑条件反应而言,并非必需.
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hydrothermal：水热
本论文阐述了利用水热(hydrothermal)法在不同条件下合成的不同形貌的ZnFe2O4、CoFe2O4、NiFe2O4纳米材料,探讨了不同条件(反应混合物PH值、反应时间、反应温度)对产物形貌的影响,研究了不同形貌的材料的气体敏感性能的差异,
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instrumental conditioning：手段式条件反应
instantaneous cardiac reserve 瞬时心力贮备 | instrumental conditioning 手段式条件反应 | insulin 胰岛素
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acid reaction：酸反应
acid conditioned reaction 酸条件反应 | acid reaction 酸反应 | acid reflex 酸反射
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acid conditioned reaction：酸条件反应
acicula 针刺 | acid conditioned reaction 酸条件反应 | acid reaction 酸反应
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conditioned response：条件反应
条件反应(conditioned response)可分为两大范型:一是经典条件反射,最初由俄国生理学家巴甫洛夫发现并做了详细研究;二是操作条件反应,又称工具性条件反应,由美国著名行为主义心理学家斯金纳(B.F.Skinner)发现并做了详尽研究.
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anticipatory conditioned response：先期条件反应
anticipatory behavior 预期行为 | anticipatory conditioned response 先期条件反应 | anticipatory control 先期控制
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delayed conditioned response：延迟条件反应
delayed conditioned reflex 延迟条件反射 | delayed conditioned response 延迟条件反应 | delayed conditioning 延迟条件作用
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conditioned emotional response：条件性情绪反应
conditioned emotional reaction 条件性情绪反应 | conditioned emotional response 条件性情绪反应 | conditioned escape response 条件性逃脱反应
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conditioned escape response：条件性逃脱反应
conditioned emotional response 条件性情绪反应 | conditioned escape response 条件性逃脱反应 | conditioned excitation 条件性兴奋