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Genetic analysis were carried out to identify powdery mildew and strip rust resistance genes in the F2 of Am6-4 amphiploid and wheat variety Jinan17. Results showed that resistances to powdery mildew and stripe rust were controlled by a single dominant gene respectively. 124 SSR primers from genome D were used for marker analysis, marker Xgwm98 150(150为下标) from the chromosome 6D was found to be linked to the new powdery mildew resistance gene with a linkage distance 20.42 cM; A special DNA band was amplified by primere xgwm33 in resistant stripe rust plants, resistance gene for stripe rust was localized on chromosome 1D, and the genetic distance between resistance gene and marker is 8.0 cM.
利用Am6-4与济南17F2分离群体进行白粉病和条锈病抗性基因的遗传分析结果证明,Am6-4中的抗白粉病和抗条锈病基因均为单显性基因;以124对D基因组SSR引物进行标记分析,引物Xgwm98在抗白粉病DNA池和单株中能扩增出特异标记带,标记与抗白粉病基因间的遗传距离为20.42cM,并将抗白粉病基因定位于6D染色体;引物Xgwm33能在抗条锈病DNA池和单株中扩增出特异标记带,标记与抗条锈病基因间的遗传距离为8.0cM,并将抗条锈病基因定位于1D染色体。
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The invention relates to the isolation and preparation of an active fraction from plant Cuminum cyminum, its further purification and standardization as chemically defined entity with their intended use as drug bioavailability enhancer for the drugs belonging to therapeutic categories such as antimicrobial, antifungal, anti-viral, antitubercular, antileprosy, anti-inflammatory, antiarthritic, cardiovascular, antihistaminics, respiratory distress relieving drugs, immunosuppressants, anti-ulcerogenic, anti-cancer, CNS drugs, corticosteroids, nutraceuticals in compositions to be administered orally/parenterally, topically, inhalations, rectally, vaginally in human beings and/or veterinary conditions.
本发明涉及植物孜然芹中的一种活性级分的分离和制备,进一步的纯化以及它作为化学确定实体的标准化,和它作为治疗类药物的生物利用率增强剂的特定用途,这类药物包括如抗微生物剂、抗真菌剂、抗病毒剂、抗结核药、抗麻风病药、抗炎药、抗关节炎药、心血管类药、抗组胺药、呼吸窘迫缓解药、免疫抑制剂、抗溃疡药、抗癌药、CNS药、皮质甾类药、营养制品,它们以组合物的形式通过口服/肠胃外,局部,吸入直肠,阴道施用于人和/或兽医学病症中。
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Antibodies including mouse antihuman neurofilament,rabbit anti-human myelin basic protein,rabbit anti-human Al,rabbit anti-human IgM,mouse anti-human IgG,mouse anti-human CD31,mouseanti-human GFAP,mouse anti-human Ki-67,rabbit anti-human serotonin(5-HT),rabbit anti-human SYN,and mouse anti-human c-fos protein were used for immu-nohistochemistry with S-P method.The colorant was brown yellow DAB.
免疫组化染色用S-P法,一抗分别为鼠抗人神经丝蛋白、兔抗人髓鞘碱性蛋白、兔抗人Al、兔抗人IgM、鼠抗人IgG、鼠抗人CD31、鼠抗人GFAP、鼠抗人Ki-67、兔抗人血清素(5-HT)、兔抗人SYN及鼠抗人C-fos蛋白,显色剂为棕黄色DAB。
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The author is combination particular text and inquiry her oomph and the style of coprology from three path: first counter Zhang kangkang's style of coprology and personality have import effect and winding life story march tease and staple and generalize of humanness's stage; Secondly from novel and prose to assay her oomph of against; Lastly from eyeshot and thinking module of person and setting of person's combination and artistic conception to discuss her taste style.
本文中,笔者结合张抗抗具体的文本,从三个方面切入研究张抗抗独特的精神品质和文学风貌:首先对在张抗抗日后文学风格及个性形成产生重要影响的曲折的人生经历进行了一次综合地梳理,并对在此基础上形成的对人的认识的不同阶段进行分类、概括;第二,分别从小说和散文里,分析张抗抗抗争的精神品质;最后,从叙述视角、"人"的思维模式、人物关系的设置、意境的营造等方面,探讨张抗抗的审美特征。
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The structure parameter influences on lateral stability, such as arch axis coefficient, arch high span ratio, crankle rigidity ratio of arch rib, vertical and lateral rigidity ratio of arch rib, lateral rigidity of bridge decking and arch rib ratio, crossbeam number, rigidity of crossbeam number the effect of non-orientedly conservative loadings of bridge deck and width span ratio, were discussed. Corresponding numerical charts were given, and they may be of some reference value and helpful to the parameter design of ribbed arches.
考虑了拱轴系数、矢跨比、拱肋抗扭刚度与横向抗弯刚度比、拱肋的竖向抗弯刚度与横向抗弯刚度比、横系梁在拱肋切平面内的抗弯刚度和拱肋的横向抗弯刚度比、横系梁在拱肋径向的抗弯刚度与拱肋的横向抗弯刚度比、桥面系的横向抗弯刚度与拱肋的横向抗弯刚度比、横系梁的数量、桥面系横向刚度引起的非保向力效应及拱肋间距与跨径的比对组拼拱横向稳定的影响,并给出了相应的图表,为拱桥设计时拱肋的结构参数的选取提供一定的依据。
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Prepare 131I-labbelled anti-CD20 monoclonal antibody using lodogen iodine labelling;(2)Measure the immunity activation of labbelled antibody by means of both cell combine analysis and LDH cytotoxicity detection kit;(3)Evaluate the injury rate of tumor exposed to 131I-chimeric anti-CD20 monoclonal antibody in vitro. The MTT method and the experiment of cell growth control are used;(4)Record the apotosis of Daudi cell using gelose electrophoresis;(5)Learn the inhibition effect of radioactive medication on the marrow. Radiohnmunoimages are taken on various intervals after injection of the labeled antibodies to the nude mice. The distribution of radioactive medication, I31l-labeled antibodies, in the marrow tissue indicates the inhibition effect. Here a B cell non-hodgkin lymphoma model in nude mice is established by us;(6)28-day observation are done in 3 groups of nude mice model.
(1) 用lodogen标记法制备131I-国产人鼠嵌合抗CD20单抗;(2)采用细胞结合分析法和乳酸脱氢酶法细胞杀伤性实验测定131I标记后国产人鼠嵌合抗CD20单抗与靶细胞的免疫活和利结合能力;(3) MTT法和细胞生长抑制实验测定131I-国产人鼠嵌合抗CD20单抗的体外杀瘤活性;(4)用琼脂糖凝胶电泳法研究131I-国产人鼠嵌合抗CD20单抗致Daudi细胞凋亡;(5)建立荷人B细胞淋巴瘤移植瘤裸鼠模型,应用γ计数法检测注射到荷瘤裸鼠体内的131I-国产人鼠嵌合抗CD20单抗的组织分布情况,明确其靶向性;(6)将荷瘤裸鼠分3组进行放射免疫治疗,分别为阴性对照组、131I-国产人鼠嵌合抗CD20单抗组、国产人鼠嵌合抗CD20单抗组。
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objective to isolate and purify the antimicrobial peptides with anti-toxoplasma gondii activity isolated from the haemlymph of musca domestica larvae.methods the antimicrobial peptides of musca domestica larvae were induced by infection and injury were isolated and purified by trituration,centrifugalization and column chromatography.then the antimicrobial peptides with anti-toxoplasma activity were sieved by mtt colorimetric method and haemacytometry.results it was found that two antimicrobial peptides had anti-toxoplasma activity through resource s cationic column chromatography and superdex g75 gel column chromatography.conclusion the two antimicrobial peptides with anti-toxoplasma activity existing in the haemlymph of musca domestica larvae had different anti-toxoplasma effect.
作者单位:山西医科大学寄生虫教研室,太原 030001;化学生物学与分子工程教育部重点实验室山西大学生物技术研究所目的从家蝇幼虫血淋巴中分离纯化具有抗弓形虫作用的抗菌肽。方法通过损伤加感染的方法诱导家蝇幼虫大量表达抗菌肽,然后经过研磨、离心和层析等过程,将家蝇幼虫抗菌肽进行分离纯化,采用四甲基偶氮噻唑蓝比色法和血细胞计数法筛选对弓形虫速殖子有抑制作用的抗菌肽。结果经resource s阳离子柱和superdex g75凝胶柱层析后,筛选出2种对弓形虫速殖子有杀伤作用的抗菌肽。结论家蝇幼虫血淋巴中存在抗弓形虫作用的抗菌肽,而且不止一种,但其效果有所不同。
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The investigation also showed that, the antioxidants with same action mechanism, for example, as both radical scavengers, phenol and amine antioxidants are synergistic; but the synergistic effects between two antioxidants with different action mechanism, such as radical scavenging phenol and amine antioxidants, and hydroperoxide decomposing ashless dithiocarbamate, are much stronger, and the heterosynergism between dithiocarbamate and alkylated diphenylamine is outstanding, which can serve as high temperature ashless high performance antioxidant combination; the heterosynergism among three antioxidants with three different action mechanisms, for example, the tri-component combination of alkylated diphenylamine, zinc dithiocarbamate and organic molybdate ester, can achieve excellent antioxidation performance.
研究还表明,具有相同抗氧机理的不同抗氧剂,如酚类和胺类抗氧剂,具有协同作用;不同作用机理的两抗氧剂之间,如自由基清除剂和氢过氧化物分解剂,具有更好的抗氧化协同效果,其中,无灰氨基甲酸酯与烷基化二苯胺抗氧剂具有最佳的抗氧协同效能,可成为高温无灰抗氧剂应用的一个很好选择;不同抗氧机理的三种抗氧剂复合,如烷基化二苯胺,氨基甲酸锌和有机钼酸酯组成的三元复合抗氧剂,具有更为优秀的抗氧化性能。
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In cards and membership cards, not only in black and white printed on them, or you can color business card printing and membership card making, cards and membership cards making machines, Business card printing and membership card making object read in an informal, information may be made by wire to a surface and even can rotate 360 ° and fault correction features that make suffered defaced or damaged barcodes reversal may be recognized, misreading rate is 6 1 0 0 in a few cases, 2-dimensional barcode also has stock in ensuer megasporogenesis,抗损, high safety may fax and photocopying, information hiding, and go with the product information.
在制卡和会员卡制作上,不仅可在白纸上印黑字,也可以彩色制卡和会员卡制作,对制卡和会员卡制作机器、制卡和会员卡制作对象不拘,资料的读取可由线到面甚至可以旋转360°,并可采用故障订正功能,可使遭受污损或破损的条形码复原识别,误读率为6 1 0 0 万分之几,二维条形码还具有储存量大、抗损性强、安全性高、可传真和影印、资料隐蔽性好、信息随着产品走等特点。
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Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.
针对本实验目的,我们设计了以下的实验步骤﹔(1)二次代谢物回收及检测样本处理:我们将发酵所得的培养上清液,利用疏水吸附性树酯Amberlite XAD-2吸附回收,并以甲醇溶离及真空减压浓缩脱水等方式处理,以取得提供抗氧化活性筛选之检测样本;(2)抗氧化活性检测及菌种筛选:以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用;经此筛选程序,我们选获了具有抗氧化物质高生产力的菌株,命名为AMBL-029C;(3)抗氧化物质的分离纯化:针对生产菌株的发酵回收处理液,以矽胶薄层色层分离法经物质层析纯化后,并以高效能液相层析法(High performance liquid chromatography;HPLC)分析物质可得一初级纯化物质,命名为AMBL-029C-TS;(4)抗氧化物质的物理化学性质分析:由酸碱转溶(acid-base fractionation)实验得知,此抗氧化物质属於中低极性的强酸性物质,对温度(37℃-100℃)及酸碱度(pH3.0-13.0)均表现出高稳定性;(5)在抗氧化机制探讨方面,我们针对数种不同的抗氧化机制进行探讨,即: 1。
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Anti-AIDS drugs:抗愛滋病藥 抗艾滋病药
anti-AIDS agents 抗愛滋病藥 抗艾滋病药 | anti-AIDS drugs 抗愛滋病藥 抗艾滋病药 | anti-allergic agents 抗過敏反應藥 抗变态反应药
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antiknock:抗爆抗爆剂抗爆的
antiknock quality 抗爆性 | antiknock 抗爆抗爆剂抗爆的 | antiknocking 抗爆的
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antimalarial agents:抗瘧疾藥 抗疟(疾)药
antileproatic 抗麻瘋藥 抗麻疯药 | antimalarial agents 抗瘧疾藥 抗疟(疾)药 | antimalarials 抗瘧疾藥 抗疟(疾)药
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HRA:抗心肌抗体
及18例健康献血员对照血清同时应用免疫荧光法检测抗核抗体(ANA)、抗平滑肌抗体(SMA)、抗线粒体抗体(AMA)、抗胃壁细胞抗体(PCA)、抗心肌抗体(HRA),应用酶联免疫吸附试验(ELISA)检测抗肝肾微粒体抗体(LKM)及抗线粒体M2亚型抗体,
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shear strength parameter:抗剪强度抗剪强度参数
抗剪强度抗剪强度shear strength | 抗剪强度抗剪强度参数shear strength parameter | 抗剪强度抗剪强度有效应力法effective stress approach of shear strength
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antiacid:抗酸剂n.抗酸剂抗酸的
antiacid additive 抗酸添加剂 | antiacid 抗酸剂n.抗酸剂抗酸的 | antiacid 抗酸剂抗酸的
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antidysenteric:抗痢疾藥 抗痢疾药
antidiuretic 抗利尿藥 抗利尿药 | antidysenteric 抗痢疾藥 抗痢疾药 | antidyskinetic 抗運動障礙藥 抗运动障碍药
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antihemorrhagic:抗出血藥 抗出血药
antihemophilic 抗血友病藥 抗血友病药 | antihemorrhagic 抗出血藥 抗出血药 | antihepatitis drugs 抗肝炎藥 抗肝炎药
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antimaniac;antimanic drugs:抗躁狂症藥 抗燥狂药
antimalarials 抗瘧疾藥 抗疟(疾)药 | antimaniac;antimanic drugs 抗躁狂症藥 抗燥狂药 | antimanic; antimaniacal 抗躁狂藥 抗躁狂药
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antimanic; antimaniacal:抗躁狂藥 抗躁狂药
antimaniac;antimanic drugs 抗躁狂症藥 抗燥狂药 | antimanic; antimaniacal 抗躁狂藥 抗躁狂药 | antimetabolites 抗代謝藥 抗代谢药