囊胚的

基本解释 (translations)

blastular

与囊胚的相关的网络例句 [注：此内容来源于网络，仅供参考]

By statistic analysis, there were significant differences with the average cell number of each morulae, early blastula and blastula between in vivo and in vitro, there were no differences with the hatching of blastula between in vivo and in vitro.

经统计分析体内桑椹胚、早期囊胚、囊胚的每一胚胎平均细胞数与体外桑椹胚、早期囊胚、囊胚的每一胚胎平均细胞数有显著差异；扩张囊胚的体内，体外每一胚胎平均细胞数没有显著差异。

The results showed that 1 there were no significant differences in the rates of cytoplast protrusion and enucleation between oocytes that were incubated in colchicine (0.4 μg/mL) for 0.5 h and oocytes that were incubated in colchicine (0.4 μg/mL) for 1 h, and the rate of cytoplast protusion can be 85.4% while the rate of cytoplast enucleation is 100%. 2 There was no significant difference in oocyte enucleation between oocytes treated with medium containing 0.2 μg/mL colchicine for 0.5 h and oocytes treated with medium containing 0.4 μg/mL colchicine for 0.5 h. 3 A maturation time of 18–23 h did not affect the rates of cytoplast protusion and enucleation by chemically assisted enucleation, whereas the rate of enucleation of oocytes by blind enucleation was found to decrease with a prolonged incubation time. 4 The development rates of reconstructed embryos could not be influenced by these two enucleation methods, increased from oocytes matured for 21–23 h.

结果表明： 1 卵母细胞在0.4 mg/mL的秋水仙素溶液中分别孵育0.5 h和1 h，胞质突起率和去核率没有显著的差异，突起率可高达85.4%，去核率达到100%； 2 0.2 mg/mL或0.4 mg/mL秋水仙素溶液将卵母细胞处理0.5 h，对去核效果没有显著影响； 3 对于体外成熟18~23 h的卵母细胞，随着成熟时间的延长，盲吸法的去核率降低，但没有影响秋水仙素诱导胞质突起的比率和去核率； 4 两种去核方法对重构胚的发育没有产生显著影响，但成熟21~23 h卵母细胞重构胚囊胚的发育率显著高于成熟18~20 h卵母细胞重构胚囊胚的发育率。

These observations show that reprogramming is easier in interspecific embryos reconstructed with ES cells than that reconstructed with somatic cells, and that ES cells have the higher ability to direct the reconstructed embryo development normally than fibroblast cells. Oocytes were reconstructed with outbreeding Kunming albino mouse ES cells and enucleated rabbit oocytes, and the effects of the passages of ES cells and 6-DMAP on the development of interspecific reconstructed oocytes were analyzed. The interspecific reconstructed ES-rabbit oocytes were activated either by combined two set electric pulses and 6-DMAP or by two set electr

以上结果显示，6－DMAP能增加胚胎干细胞异种重构卵的卵裂率，对重构卵囊胚的发育率影响不大；高培养代数和低培养代数的胚胎干细胞用于异种核移植不影响异种重构卵的卵裂率和囊胚发育率；用胚胎干细胞作为供核细胞；比体细胞作为供核细胞所构建的异种重构胚更容易进行重编程，并且胚胎于细胞指导异种克隆胚胎正常发育的能力强于体细胞。

Using the Hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which Sry transcription can be detected failed to yield any positive results of Xist. Female embryos at the pronucleus stage and 2-cell failed to produce any positive result of Sry and Xist too.

然后利用实验一确定的PCR条件，以Hprt为阳性对照，用巢式RT-PCR对小鼠早期胚胎进行Xist基因的转录分析，结果发现，转录Sry基因的睾丸组织以及雄性胚胎，从受精卵发育到囊胚的过程中，基本上不转录Xist基因；不转录Sry基因的雌性卵母细胞和雌性胚胎，从出现原核开始，到发育至2-细胞期的过程中，Xist基因一直不转录，但是，从4-细胞期开始，一直到孵化前囊胚阶段，雌性胚胎都转录Xist基因。

After Cryopreservation of embryo and larvae of manina clam Ruditapes variegata, the results indicate the survival rate of blastula embryo and vlegier are significantly better than that of trochophore and D-shaped larvae(P.05); the survival rate of blastula embryo is 42.3%(4.0M DMSO) and the effect of high sucrose density is better than that of low sucrose density with the same DMSO dendity.

对杂色蛤胚胎和幼虫的超低温冷冻保存发现，杂色蛤囊胚和面盘幼虫的保存复活率显著高于担轮幼虫和D形幼虫的复活率(P＜0.05)；杂色蛤囊胚的解冻复活率达42.3％(4.0M DMSO)，相同DMSO浓度条件下，高蔗糖浓度组保存效果好于低蔗糖浓度组，但差异不显著(P＞0.05)；在相同蔗糖浓度条件下，抗冻液中DMSO浓度较高组的保存效果好于DMSO浓度较低的组，但差异不显著(P＞0.05)。

With the prolonging of treatment with 6-DMAP,the developmental ability of parthenogenetic embryos decreased.

随着6-DMAP处理时间加长，孤雌胚的发育能力降低。6-DMAP处理1小时的桑葚胚／囊胚的发育率(72％)远远高于6-DMAP处理6小时的桑葚胚／囊胚的发育率(28％)。

In the experiment, the FSH+LH combinations were adopted to superovulate KM mice to ascertain the proper phase to gain morulae and blastocysts, three ways were adopted to split the morulae and blastocysts, demi-embryos were cultured in D-PBS, mTCM199, CZB, mM16 added with FCS or MS of different concentration, and transplantation of the blastocysts derived from demi-embryos was performed.

本试验以昆明白小鼠为实验动物，试采用FSH和LH进行超排处理，确定了该处理程序下获取桑椹胚、囊胚的恰当时间，比较了三种方法对桑椹胚和囊胚分割的效果，采用了添加不同浓度FCS、MS的4种培养基对分割胚进行体外培养，并进行了半胚移植。

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分为4个部分，第一部分对影响猪卵母细胞体外成熟和孤雌激活后胚胎分裂的几个重要条件进行了比较研究，确立了一种较好的培养方法：与颗粒细胞共培养，找到了一种适合猪卵母细胞体外成熟的培养基：mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸，成熟率和分裂率分别为(86.7±3.35)%和(86.3±4.16)%，国外报道的最高成熟率为(85.7±4.1)%；第二部分对猪卵母细胞孤雌激活的化学方法进行了研究，确立了化学激活猪卵母细胞的两种最佳方法：1将成熟的去卵丘颗粒细胞的MII期卵母细胞用20μmol/Lionomycin作用30min，再将卵母细胞培养于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培养液中，卵裂率和桑囊胚发育率达到(76.7±7.6)%和(37.1±6.4)%2将成熟的去卵丘颗粒细胞的MII期卵母细胞在200μM/L的Thimerosal中处理20min，再与8mM的DTT共孵育30min，卵裂率和桑/囊胚形成率为(81.0±2.8)%和(39.6±2.7)%；第三部分对孤雌激活胚胎的培养条件进行了研究，确立了一种最佳的胚胎培养条件：在SOF简单培养基中添加颗粒细胞进行前3天的培养，然后转入添加胎牛血清的NCSU—23培养基并和输卵管上皮细胞进行后期的培养，其桑椹胚和囊胚的发育率为(59.5±3.2)%；第四部分研究了IGF-I

Following chemical activation, blastocysts rate of the treated oocytes was similar to untreated oocytes.8 Following fertilization, however, few oocytes inhibited with CHX developed into morulae/blastocysts, due to a high incidence of polyspermy.9 Cortical granule migration occurred during inhibition, but CHX inhibition impaired CG migration, significantly no oocytes inhibited by CHX completed CG migration after maturation.10 CHX inhibition had no effects onα-microtubles and microfilaments of goat oocytes.

抑制24h转为正常培养24h，不影响卵母细胞的成熟和孤雌激活能力，并且CHX抑制后再成熟的卵母细胞经孤雌激活发育到囊胚的比例与对照组卵母细胞相似。8、体外受精后，CHX抑制后再成熟卵母细胞的多精受精现象显著增加，发育到桑椹胚/囊胚的比例显著低于对照组。9、CHX抑制过程中皮质颗粒仍能发生迁移，但是CHX抑制会对皮质颗粒的迁移造成不可恢复的损伤，使再成熟的卵母细胞内皮质颗粒不能完全迁移。10、CHX抑制对山羊卵母细胞α-微管和微丝都没有影响，无论是抑制后处于生发泡期的卵母细胞，还是抑制后再成熟的卵母细胞，微管和微丝的分布都与对照卵母细胞相似。

The average cell number of each embryo were significant differences between 12-24 hr and 36-48 hr treatment and 0-72 hr treatment of early blastula; the cleavage index of each embryo were significant differences between 0-12hr and 24-36 hr treatment and 0-72 hr treatment of the hatching of blastula; embryo cell number and cleavage of other treatments were no differences.

早期囊胚的12-24h处理组和36-48h处理组的每一胚胎平均细胞数与0-72h处理组有显著差异；扩张囊胚0-72h处理组的分裂指数与0-12h处理组和24-36h处理组有显著差异，其它各组的胚细胞数和分裂指数均无差异。

与囊胚的相关的网络解释 [注：此内容来源于网络，仅供参考]

amphiblastula：两极囊胚;空囊胚

\\"端卵的\\",\\"amphiblastic\\" | \\"两极囊胚;空囊胚\\",\\"amphiblastula\\" | \\"大壶螺超科\\",\\"AMPHIBOLOIDEA\\"

blastocoele：囊胚腔

开始时囊胚是由约1000个细胞组成的,球状中空结构,球腔即为囊胚腔(blastocoele),球壁由单层细胞组成,植物极(Vegetal pole,可以看作是日后海胆身体的尾部)上皮比较厚,出现细胞内馅的倾向.

blastocyst：(囊)胚泡[见于哺乳动物着床前的胚囊]

blastocoel 囊胚腔 | blastocyst (囊)胚泡[见于哺乳动物着床前的胚囊] | blastoderm 胚层;胚盘

blastoderm：胚盘 囊胚层

blastocytoma 胚细胞瘤 | blastoderm 胚盘 囊胚层 | blastodermal 胚层的 胚盘的

blastoderm;blastodisk：(蛋黃上的)胚盘;胚膜;囊胚层

blastpump送风泵 | blastoderm;blastodisk(蛋黃上的)胚盘;胚膜;囊胚层 | Blastomyces芽生菌

amphiblastic：两极囊胚的

amphibivalent 双二价体 | amphiblastic 两极囊胚的 | amphibolic metabolic pathway 无定向代谢途径(中心代谢途径)

blastosphere：囊胚,囊胚泡

blastopsephitic | 变余砾状 | blastosphere | 囊胚囊胚泡 | blastous | 胚的芽的

blastular：囊胚的

囊泡形成vesiculation | 囊胚的blastular | 囊胚腔blastocoele

stereoblastula：实原肠胚;夭囊胚

在粪上生活的 stercoricolous | 实原肠胚;夭囊胚 stereoblastula | 实体感 stereognosis

blastocoel：囊胚腔

桑椹胚一方面继续地分裂,一方面中心的细胞渐向表面迁移,因而变成一个空心的圆球,此时期的胚胎称囊胚(blastula),中间的空腔内充满胶状液体,称囊胚腔(blastocoel).