tRNA
- tRNA的基本解释
-
-
(=transfer RNA)转移RNA
- 更多网络例句与tRNA相关的网络例句 [注：此内容来源于网络，仅供参考]
-
Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.
maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。
-
Four types of start codons were identified in the 13 protein coding genes, in which TAA and TAG were used in 12 PCGs as stop codons, except ND5, Most tRNA genes could form typical secondary structures except tRNA, which had an absence of the DHU arm.
除tRNA外，其余21个tRNA的二级结构均属典型的三叶草结构。tRNA的DHU臂缺失，在相应的位置上只形成一个环。
-
However, the recent discoveries are overturning the traditional opinions. Nuclear-born tRNA can be exported out of nucleus, while cytoplasmic-residing tRNA can also be retrogradely transport into nucleus.
然而，最近的研究成果在颠覆传统观念，tRNA不但能被转运出核，还能被逆向转运入核。2008年，新概念&tRNA核质动态分布&(tRNA nuclear-cytoplasmic dynamics)被提出，取代tRNA核质运输，描述tRNA在细胞核、质间的流动。
- 更多网络解释与tRNA相关的网络解释 [注：此内容来源于网络，仅供参考]
-
entry site：进入位点[如核糖体进入位点,氨酰tRNA进入位点等]
entropy increase 熵增加 | entry site 进入位点[如核糖体进入位点,氨酰tRNA 进入位点等] | enucleation 去核
-
entry site：进入位点[如核糖体进入位点、氨酰tRNA进入位点等]
entropy increase 熵增加 | entry site 进入位点[如核糖体进入位点、氨酰tRNA进入位点等] | enucleation 去核
-
entry site：進入位點[如核糖体進入位點,氨 tRNA進入位點等]
entropy increase 熵增加 | entry site 進入位點[如核糖体進入位點,氨 tRNA進入位點等] | enucleation 去核