首先

First,buy in your own backyard.

首先，在你自己的后院购买。

Escherichia coli was first isolated in 1885 by the German bacteriologist, Theodor Escherich, as a normal inhabitant of the intestinal tract. Escherich named the organism Bacterium coli, the name reflecting the rod-shape of the cell (Bacterium means rod-shaped) and its intestinal habitat. The genus name Bacterium subsequently was changed to Escherichia in honor of its discoverer.

大肠杆菌于1885年首先被德国的细菌学家Theodor Escherich分离出来，因为它是一种普通的肠道居住者，Escherich把它命名为Bacterium coli，这个名字反映了细胞的杆状形态（Bacterium指的是杆形）和生活环境。

Use constituent piece to stick mural way depart to foster Niu Er to become fiber cell above all, after classics generation is rarefied, fat simple way will be contained double the skin of the 4th acting ox that outside conveying carrier to turn into body, the particularity of person bacteriolysis enzymatic mammary gland that chooses number fosters becomes fiber cell, g/mLG418 chooses 500 μ 2 weeks, g418 halve continues to develop 3 generation of 2 ～, catch the cell after choosing to undertake PCR detects to turning next reach nucleus analysis.

首先采用组织块贴壁法分离培养牛耳成纤维细胞，经传代纯化后，脂质法将含有双重筛选标记的人溶菌酶乳腺特异性表达载体转入体外培养的第4代牛皮肤成纤维细胞，500μg/mLG418筛选2周，G418减半继续培养2～3代，然后对转染筛选后的细胞进行PCR检测及核型分析。

Theodore Roosevelt first came to the badlands in September 1883 on a hunting trip.

西奥多罗斯福首先来到荒地在1883年9月在打猎。

But for all of this to happen, Baghdad must be secure.

但为了这一切的实现，巴格达首先要保持安全。

After reviewing the good sourcing management of some successful examples, detailed analysis is made in the second part on the internal and external conditions and the competition and cooperation of MAX BAHR.

本文首先论述了美饰邦公司在全球化经济格局下到中国设立采购中心的必要性和重要性，并以领先公司的采购管理特点出发，对美饰邦公司的内外环境及面临的竞争与合作进行分析，提出了结合公司特点的采购思路，主要包括美饰邦公司的联合采购策略、品牌采购策略、双赢采购策略和供应商整合等，并提出了建立有效的采购机制、高效的采购管理系统、积极推行全球采购等整合思路。

The scandal is that suspicious people were hired in the first place, says Mark Baillie, a terrorism wonk at International Policy Network, a London think-tank.

伦敦智库"国际安全网络"的恐怖主义专家马克·贝利表示，"发生的丑闻在于，可疑的人反而首先获得了雇用。"

In the fourth chapter,first of all,a metric space is derived by the Loeb measure space which is a standard measure space,follow that the Completeness,separability and the Baire theorem are expressed.

第四章中，首先由Loeb测度这个标准测度空间导出度量空间，接着讨论了该度量空间的完备性和可分性以及其Baire定理(来源：A8525B52C论文网www.abclunwen.com)。

First and foremost, is that baiting enough to encourage one to make do with less sleep?

首先的也最重要的是，鼓励人家用较少的睡眠勉强撑着过是件值得一做的好事吗？

Well, first of all I want two boxes of baked beans.

嗯，首先我要两箱烤豆。

I'm strongly against the death penalty — it's an eye for an eye.

我不赞成死刑——这是以牙还牙的报复行为。

And to get you the support you need, we're enlisting all elements of our national power: our diplomacy and development, our economic might and our moral suasion, so that you and the rest of our military do not bear the burden of our security alone.

并给你们所须的支援，我们正徵召国家所有各种的力量：我们的外交及发展，我们的经济力量与道德劝说，所以你们与其他军人不须要孤独地负起国家安全的责任。