间接获得的

This is because native C++ RTTI values are retrieved through an additional indirection on almost all platforms.

这是因为在几乎所有平台上，原生的 C++ RTTI 是通过一个额外的间接层来获得。

In order to develop an indirect ELISA for diagnosing bovine paratuberculosis, the DNA fragmentsof map086 2and map2154c were amplified from the genome DNA Mycobacterium avium Subspeciesparatuberculosis K10. Then the DNA fragments of map0862 and map2154c were spliced byoverlapping extension, yielding a fusion gene map0

为了建立一种有效的牛副结核病间接ELISA方法，本研究设计了两对引物，从副结核分枝杆菌P_(18)株的基因组中PCR扩增出两个目的基因map0862和map2154c、，采用重叠延伸剪接技术(splice by overlapping extension，SOE)获得融合基因map0

Finally, the enterprise of business of relevant share certificate also it is good that hopeful acquires indirect interest.

最后，相关参股券商的企业也有望获得间接利好。

Outflows of high-velocity gas were studied, indirect correlations between outflow mass and bolometric luminocity were obtained.

对高速气体外流进行研究，首次获得外流质量和中心源热光度的间接相关。

Convey albumen immunity with rarefied LTb 8 Zhou Ling BALB/c rat 3 hind, after taking lienal cell and cell of SP2/0 bone myeloma to undertake confluence, undertake filtration with indirect ELISA and finite and attenuant law, obtain 4 individual plant to be able to stabilize the individual plant of cross tumour cell that secretes antibody of Chan Kelong of LTb of bacili of bowel of the Chinese People's Anti-Japanese Military and Political College, antibody of odd clone of this individual plant names for 1F2, 2C2, 3D6, 4H6 individual plant.

用纯化的LTb表达蛋白免疫8周龄BALB/c鼠3次后，取脾细胞和SP2/0骨髓瘤细胞进行融合后，以间接ELISA和有限稀释法进行筛选，获得4株能稳定分泌抗大肠杆菌LTb单克隆抗体的杂交瘤细胞株，该株单克隆抗体命名为1F2，2C2，3D6，4H6株。

The intracellular expression of viral gene and phenotypical change of transformants were examined.

结果：分离获得了可长期体外培养的SV40转化细胞克隆，已传23代以上，体外存活超过250d；DNA印迹实验显示SV40大T基因已整合至细胞染色体内，间接免疫荧光检测示多数细胞胞核内有大T基因产物的表达；转化细胞对裸鼠无致瘤性，并可正常表达角蛋白。

The process of eliciting, analyzing, and applying direct and indirect customer feedback to improve operational performance.

获得，分析和直接或间接的应用顾客反馈的过程，以改进运作业绩

The fusion DNA fragments of ag85b-mpb64 and ag85b-mpb64-esat-6 were obtained by PCR andSOE technique. Various DNA vaccines were constructed with the pcDNA3.1: fusion of two genes, and of three genes, bivalent combinations and trivalent combinations(pCA+pCM+pCE6). BALB/c mice were vaccinated with this DNA vaccines.The mice injected withBCG were positive control and the mice injected with pCDNA3.1 and PBS were negative control.The mice were immunized 3 times with 2-wk intervals. The animals in group BCG were only inoculatedsubcutaneously with 1×10~6 CFU BCG at initial vaccination. The serum IgG titers and IgG isotype weredetermined using iELISA coated with M. bovis PPD and rMAE protein expressed and depurated inprokaryotic expression system every week.

同样，利用PCR和SOE技术，获得牛分枝杆菌mpb64-ag85b和mpb64-ag85b-esat-6融合基因，以pCDNA3.1为载体构建了牛分枝杆菌多价组合和多基因融合DNA疫苗：二基因融合(pCDNA3.1-MPB64-Ag85B，简称pCMA)和三基因融合(pCDNA3.1-MPB64-Ag85B-ESAT-6，简称pCMAE)DNA疫苗；二价组合和三价组合(pCA+pCM+pCE6)DNA疫苗，免疫BALB／c小鼠，以牛分枝杆菌BCG免疫组为阳性对照，以pCDNA3.1及PBS免疫组为阴性对照，共免疫3次，每次间隔2周，BCG组仅初免时皮下免疫1次。1免后每周，以原核表达纯化的重组MPB64-Ag85B-ESAT-6蛋白和牛分枝杆菌PPD为包被抗原，以间接ELISA方法检测血清IgG水平及lgG亚类。

High Iodised Salt coverage has been achieved in urban and peri-urban areas and most boarding schools.

直接或间接实施人力资本开发，使贫困地区获得摆脱困境的核心能力。

For a breach of this promise, the undersigned agrees, for itself and for all the other Obligors, that, without in any manner limiting either the liability of the undersigned or your resources, the undersigned and all of the other Obligors shall forfeit all profits directly or indirectly received as a result of the work performed by the undersigned in breach of these covenants and further, that the undersigned and the other Obligors shall be liable for any direct out of the pocket costs and reasonable legal fees incurred by you in connection with the recovery of such damages.

对于违反这项承诺，同意署名，给自己和所有其他义务人，，未经任何方式限制或者责任署名或贵资源署名和所有其他义务人应放弃所有直接或间接地获得盈利由于工作署名违反这些公约及进一步署名和其他义务人，将被排除在任何直接、合理的费用口袋诉讼费用由您与回收等损失。

Next time I see you,I shall give you a black eye.

下次我看见你，我会打你一顿。

In this paper,design scheme of classification system of wood surface color is established.

提出了木质板材颜色分类的系统设计方案。