确定组

Agonistic behavior and hierarchical formation of the Przewalski's Horse was conducted from the last ten-day in May to the last ten-day in June, 2007, when a new herd was formed and released into the second reintroduction site in Kalamaili Ungulate Reserve, Xinjiang. Focal sampling and all-occurrence sampling methods were used to observe and record the agonistic behaviors of the herd, and the dominance index of each individual was calculated.

2007年5月下旬至6月下旬，针对新组群并放归于卡拉麦里有蹄类保护区第二放归点的普氏野马繁殖群，采用焦点动物取样法和全事件记录法，观测记录了组群和放归初期繁殖群内个体间的争斗行为，以优势指数法确定繁殖群内的个体等级序列。

On the base of applied tech. and system structure, the key tech. are expressed in detail. It includes:Knowledge representation technology: it is described in two stages by "Depictive frame + Rule group"."Depictive frame" is used to reflect the factors concerning question field."Rule group" reflects the son-question in question field; knowledge management system: it is made up of knowledge-base editing component, factor classifying component, knowledge inspecting component and extractive knowledge component; uncertainty reasoning adopt method of elicitation search strategy and data drive controlled by member knowledge. It take reliability of compound proof at first. That can resolve the matching contradiction. Reasoning explanation introduce pre-text and followed method of resolving path. Knowledge discovery component take SLIQ as basic arithmetic. It provides classification model in two stage forms which aim at training suit. That is decision tree and productive rule.

在确定应用技术与系统结构的基础上，重点阐述了本文的核心技术，这包括：知识表示技术，采用&描述框架+规则组&的层次结构，&描述框架&用于描述问题域所涉及的因素，&规则组&反映问题域内的子问题；知识管理系统，由知识库编辑构件、因素分类构件、知识检测构件和知识求精构件组成；不确定性推理机采用启发式搜索策略，元知识控制的数据驱动，以复合证据可信度优先作为匹配冲突消解的策略，推理解释采用预置文本与求解路径追踪的方法；知识发现，采用SLIQ快速可伸缩算法，分类模型用判定树与产生式规则两种形式描述。

Uncertainty is lack of believable knowledge or information about (1) all relevant antecedent conditions;(2) all possible outcomes; and (3a) either universal laws (governing deterministic outcomes as the only physically possible ones) or (3b) probability laws (governing indeterministic/stochastic sets of outcomes).

不确定性的意思就是缺乏可信赖的知识或资讯，这些知识或资讯是关於：(1)所有相关的先决条件；(2)所有可能的结果；(3a)可能是通则(支配确定的结果以作为唯一可能的结果)；(3b)机率法则(支配非确定的/随机的一组可能产出)。

Results showed that urokinase at 20U/ml was the optimal concentration used. Fibrinolysis kinetics and plasma plasminogen concentration of 27 normal persons and 27 patients with acute myeloblastic leukemia were detected, a significant difference was observed between the two groups. Repetition test showed that the coefficience of variation was 5.24±1.51%. This method could also be used to detect the concentration of plasma fibrinogen.

结果 初步建立了正常人纤溶动力学分析参数，确定了20U/ml尿激酶为最适试验浓度，检测22例正常人，27例急性髓细胞白血病患者纤溶动力学及纤溶酶原含量，显示患病组较正常组差异有显著意义(P.05， P.01)，重复性试验显示该方法的变异系数为(5.42±1.51)%，并可同步测定血浆纤维蛋白原浓度。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

In order to decide the safety of the virus to human,The susceptibility of Budded Virusto cell strains and mice,occuluded virusin the sheets of liver and renes was observed with microscope and electronic microscope.

为确定家蚕基因工程表达系统产品对人的安全性，观察了该病毒对哺乳动物细胞株及对小鼠的易感性，建立了检测Bm-NPV蛋白组份的dot-ELISA方法，可用于生物表达器生产的产品中残存多角体病毒组份的检测。

The time span of the Xiaochaka Formation has also been constrained by related biostratum in present paper. It is inferred that the age of volcanism in the Nadi Kangri Formation is probably occurred between the Late Triassic Norian and Rhaetian, prior to the previous age-constraint of this Formation.

运用已有的生物地层资料确定肖茶卡组地层的沉积时间(晚三叠世诺利期-瑞替期)，从而推测那底岗日组火山岩形成时代略晚于这个时间，很可能早于前人关于该套地层的时代认识。

Investigation results demonstrate that the age of intermediate to alkaline potash rich volcanic rocks of the first cycle of the Qingshan Formation is 109.9±0.6 Ma; and the age of the acidic rhyolitic volcanic rocks of the second cycle of the Qingshan Formation ...

结果表明，青山组第一旋回中偏碱性富钾火山岩的形成年龄为10 9.9± 0 。6Ma ，第二旋回酸性流纹质火山岩形成年龄为 10 8.2± 0 。6Ma ，据此确定胶莱盆地青山组火山岩应为早白垩世岩浆活动的产物。

Cycle are divided into for the phosphoric rock series of the Yangshui Formation at this zone, in association with sequence interface and its relative characterization, the types of the sequences are determined and the Yangshui Formation is considered a high-ranking I-level sequence that is constituted by a series of low-grade retrograding type of de.

应用层序地层学的概念和方法，从沉积特征研究入手，对该区的含磷岩系———洋水组划分出5个沉积层序，结合层序界面及层序的归纳描述，确定了层序类型，并认为洋水组是一套海进体系域中由一系列低级别退积型沉积层序组成的高级层序。

Make sure that the axle of the back wheel goes in between the spokes of the front wheel.

要确定后轮组的轴穿插在前轮组的辐条之间。

It is also known as one of the most poisonous naturally occurring substances.

它也被称为一个最自然发生的有毒物质。

The greatest stress is found at the location on the cross section where V is the largest.

最大应力出现在横截面上V为最大的地方。