染色于

Methods KC and FE of tympanic membrane were cultured on the surface of CCM prepared by torrefaction, then the cells biological characteristics were detected by immunofluorescence stain of keratin and vimentin, and the cells proliferative activity were identified by immunofluorescence stain of PCNA.

方法热干法制备CCM，传代的大鼠鼓膜上皮KC和FB分别接种于培养板及CCM表面，通过角蛋白、细胞膜波形蛋白免疫荧光染色鉴定细胞特异性，通过细胞核增殖抗原(proliferating Cell nuclear antigen， PCNA)免疫荧光染色测定膜上细胞的增殖能力，通过细胞培养液上清的经脯氨酸测定评价对FB胶原分泌功能的影响。

Five rats from both groups were killed at days 7,14 and 28,respectively.And we used deproteinization analysis to detect the plasma H2S concentration and hematoxylin and eosin stain and Mallory trichrome stain for the lung tissue.

观察指标：于试验的第7、14、28天时每组分别处死5只大鼠，采用去蛋白分析方法测量血浆中H2S含量，取肺组织行HE染色和Mallory三色染色。

Results as follows;(1) At 25th week of fetal age, the staining intensity and cell density almost equalled to that at birth in cervical spinal cord, brainstem, hippocampus, and vermis of cerebellum; while there are only 1/4 cell densities in cerebral cortex.

用胶质原纤维酸性蛋白抗体进行免疫组织化学染色。结果表明：(1)在颈段脊髓、脑干、海马和小脑蚓部于胚胎25周其GFAP染色强度、细胞密度接近出生时水平。

We observed the changes of the proprioceptors in the partly injuried ACL and the xenogeneic graft/artificial graft being used to reconstructing the injuried ACL with gold chlorid staining method and laser copolymerization electron micrography method to observe the morphological changes of the neuromechanism in the ligament tissue,with RT-PCR method to evaluate the proportion and the regeneration of the nerve component and with HRP retrograde tracking technology and electrophysiologic study to judge the functional changes of the neuromechanism in the ligament tissue,respectively.

本研究分别制作兔ACL部分损伤动物模型、异种肌腱移植物重建损伤ACL动物模型和LARS人工韧带重建损伤ACL动物模型，分别于术后不同时间点对损伤ACL/移植物进行氯化金染色光学显微镜检查和PGP9.5免疫荧光染色后激光共聚焦电子显微镜检查观察韧带内神经结构形态学变化，用RT-PCR法检测韧带中GAP-43和PGP 9.5的mRNA表达情况以评价损伤ACL/移植物内神经成分比例及神经再生情况，通过HRP逆行追踪技术和电生理检查(包括体感诱发电位和腘绳肌肌电图)评价损伤ACL/移植物内神经结构的功能情况。

Grade Ⅳ is that the rat circumrotates to right in automatic action.②15 rats in each group were selected. 24 hours, 48 hours, one week after operation, we opened the skulls, took out the brain and used 2,3,5-Triphenyltetrazoluim Chloride staining to measure infarction volume, hematoxylin-eosin staining to observe the pathological change , and immunohistochemistry to detect the infiltration of CD34+ cells.

将两组大鼠各取15只，分别于术后24，48 h和1周开颅取脑，用2，3，5-氯化三苯基四氮唑染色测定脑梗死体积、苏木精-伊红染色检查脑组织病理变化、免疫组织化学检测CD34阳性细胞浸润情况。

After 60 mm of ischemia/reperf黶ion, the expression of 11-8 was recognized more strongly on postsinusodial venules and sinusodial endotbelial cells,and lots of neutropblls infltrated in liver tissue. Wher~s, after 240 mm of reperllision, IL-S imniunobistochemical stainning fade more obviously than before, quantities of neutrophils sequestrated in liver leading to hepatic cells injury.

结果发现：肝缺血再灌注后60分钟，肝脏小叶中央静脉周围和肝窦状隙内皮细胞表面IL-8呈强阳性染色，此时肝组织中可见大量散在中性粒细胞浸润，于再灌注240分钟时，IL-8组化染色变淡，但分布部位与再灌注60分钟时相似。

FCM appeared obvious inferior diploid peak and blocked the cells to the G_1 phase.The red particles throughout the cytoplasm and permeated the plasma membrane could also be seen.Chromatin lining and breeding body could be seen under the transmission electron microscope.

FCM出现明显的亚二倍体峰并将细胞阻滞于G1期，Annexin-V-FITC联合法染色可见细胞凋亡的特征性贯穿胞质穿过胞膜的红色颗粒，电镜下可见染色质边集及生发小体的凋亡形态特征。

On the basis of comparative experiments of one step dyeing, dye bath diluting, dye replenishing, and researches on gradation transition technology, the progressive shade dyeing for real silk fabrics is determined by optimizing dyes, auxiliaries and equipment, thus resulting in good results.

通过一次性染色法、染液稀释法、染料补加法的对比试验，染料、助剂、设备的优选以及层次过渡技术的研究，确定真丝绸渐进色染色工艺并应用于生产，取得良好效

Expression of TGF- 1 and iNOS mRNA was higher in the control chick retina and choroid than that in FDM. The immunostaining of TGF- 1 and iNOS protein mainly was detected in the outer part of photoreceptor layer, and nonspecific immunostaining was found in the RPE and choroids.

在鸡视网膜和脉络膜组织有TGF-β1和iNOS蛋白质及其mRNA的表达，TGF-β1和iNOS的阳性染色主要分布于视网膜光感受器的外节，并且位于光感受器外节的不同位置，而在RPE及脉络膜无明显的阳性染色。

Methods The AGS cellseeded coverslips were prepared, then the two different status of Lactobacillus acidophilus and two different forms of Hp were added in different order. 2 hours later, the bacteria were Garm's stained, and the adherence of Hp to AGS cell were observed by microscope and the adherence index was calculated.

制备人胃上皮腺癌细胞系细胞爬片，将两种状态嗜酸乳杆菌和不同形态Hp菌分组按不同顺序加入，作用2 h，革兰染色后光镜观察分析Hp黏附指数产生的变化，荧光抗体染色法定性评价黏附于AGS细胞的Hp密度的改变。

Do not gum up the work by telling mother what we are going to do .

别告诉母亲我们要干的事，那会把事情搞糟的。

In recent years, application of the partial prestressed concrete beam s is developed.

近年来，随着部分预应力砼梁应用的推广，发现我国规范所采用的名义拉应力法的计算结果不稳定。