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The inhibition of micro-organism growth is most pronounced for B. subtilis and A. niger with Fraction AP3 of I. oblongifolia leaves.

对微生物生长的抑制是最具有分数和枯草芽孢杆菌的一 oblongifolia AP3黑曲霉明显叶子。

A substrate concentration of 0.277 kg/L, enzyme/ substrate level of 1408 U/g, temperature of 39.2 ℃, and a hydrolysis time of 4.86 hour were found to be the optimum conditions for high antibacterial activity. Under this condition, the bacteria Escherichia coli, Micrococcus luteus and Bacillus subtilis in the hydrolysates had the inhibitory rate of 71.40%, 56.88%, and 64.54%, respectively.

经Design Expert 7.1.2软件优化,得出胃蛋白酶酶解鲶鱼骨蛋白的最优条件为:底物浓度0.277kg/L,加酶量1408 U/g,水解温度39.2℃,水解时间4.86 h,在此条件下获得的水解物,对大肠杆菌、藤黄微球菌及枯草芽孢杆菌的抑菌率分别为71.40%、56.88%和64.54%。

The result shows that 4.1-6.5 kDa antibacterial substances separated from the crude extract described above could efficiently kill Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis and Micrococcus luteus), but no any activity for Gram--negative bacteria or fungi.

可产生出分子量在4.1-6.5KD之间的抗菌物质,这些抗菌物质对革兰氏阳性菌(金黄色葡萄球菌、藤黄微球菌及枯草芽孢杆菌)有广泛的抗性,而对革兰氏阴性菌和真菌没有抗性。

Two strains of microzyme and Bocillus subtilis could be selected to produce high quality protein feedstuff from soybean meal by solid state fermentation.

结论]选择酵母菌和枯草芽孢杆菌2种菌作为豆粕固态发酵生产优质高蛋白饲料的复合菌种。

ConclusionTwo strains of microzyme and Bacill us subtilis could be selected to produce high quality proteinfeedstuff fromsoybean meal by solid state fermentation.

结论选择酵母菌和枯草芽孢杆菌2种菌作为豆粕固态发酵生产优质高蛋白饲料的复合菌种。

Results:The results showed that the extraction of Milpa Alta can obviously inhibit the growth of the Staphylococcus aureaus、 Hemolytic B Streptococcus、 Proteus species、Bacillus subtilis、Bacillus cereus、Staph.Epidermidis、Escherrichia coli andP.aeruginosa.

结果:"米邦塔"仙人掌汁对金黄色葡萄球菌、大肠杆菌、绿脓杆菌、乙型溶血性链球菌、变形杆菌、枯草牙孢杆菌、蜡状芽孢杆菌、表皮葡萄球菌均有明显的抑制作用。

The results showed that vanillin had the inhibition effect intensively, the minimum inhibition concentration of vanillin against E. coil was 0.23%, Bacillus subtilis was 0.26%, Saccharomyces was 0.14%, and Aspergillus was 0.14% and Mucor rouxii was 0.03%.

结果表明,香兰素有较强的抑菌性,对大肠埃希菌的最低抑制浓度为0.23%,枯草芽胞杆菌为0.26%,酵母菌为0.14%,黑根霉和毛霉分别为0.14%和0.03%。

The results showed that the bacteria B7 came into the logarithmic growth phase for 5 h culture. Both the fermentation liquid and its filtration could inhibit C. colhounii effectively, and the fermentation of dilution l0 times also showed significant differences compared with the control. The inhibiting effect of its fermentation media filtration was even more significant in the optimizing test, with the maximal inhibition rate of 100%. The best fermentation condition was 0.5% glucose, 0.1% ammonium muriate, without extra mineral, pH 5.4, temperature 28℃. Based on the morphological properties and 16S rRNA sequence, the bacterium B7 was identified as the Bacillus subtilis.

结果表明,该菌体在培养了5h后即进入对数生长期;B7细菌的发酵液原液和无菌滤液均能抑制病原菌的生长,其中发酵液原液的10^3稀释液的抑菌效果仍与对照之间存在显著的差异;发酵条件优化试验中发现该细菌的无菌滤液最大抑菌率达100%,最佳发酵条件为葡萄糖0.5%、氯化钱0.1%、不加无机盐、pH5.4,温度28℃;结合形态学特征和16S rRNA的序列分析,将该菌株鉴定为枯草芽孢杆菌。

Result There are sterile growth of film culture of bacillus subtilis of central paraffin oil and directin paraffin oil sampling.

结果石蜡油中心的枯草芽孢菌片培养及石蜡油直接取样培养全部无菌生长。

One pectate lyase producing strain, ZQX8, was separated from the salina soil in Yancheng National Grus japonensis Nature Reserve, Jiangsu Province. Through modality of bacteria, specialty in physiologic and biochemical, and 16S rDNA, we identified the bacteria to be Bacillus subtilis.

从江苏省沿海滩涂国家丹顶鹤自然保护区盐碱土样中,分离得到一株果胶酸裂解酶产生菌ZQX8,经形态、生理生化特征和16SrDNA鉴定,初步鉴定为枯草芽孢杆菌。

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