杆状

The main body of a book adopt the MRS culture medium flat to sift a lactobacillus , separate a purification by that 4 time of the above biography replaces it with reaching in a row,shape and rhabditiform the mirror is observed to hammer when checking up two kinds, the hammer shape is that the circle steeps a shape , become a pair or link up , assume strong point muchly catenoid existence; Long in being slender rhabditiform rhabditiform , strong arrangement of chain, large amount o'clock presents to send out the filiform congeries.

摘要本文采用MRS培养基平板筛选乳酸菌，经过连续4次以上的传代，以达到分离提纯，镜检时观察到链球状和杆状两种，链球状为圆泡状，多成对或串连，呈长链状存在；杆状为中长细杆状，链壮排列，大量时呈发丝状堆积。

H. pylori is usually spiral-shaped, it can turn from rod form to coccoid shape after prolonged in vitro culture or antibiotic treatment.

幽门杆菌的菌体形态以螺旋杆状为主，但体外培养时间过长或是在抗生素处置的情况下，会造成菌体的形态由杆状变成球状。

RESULTS Hp can grow in liquid media in room temperature, and 3 out of 18 strains of coccoid Hp reverted to bacterial.

结果 Hp可在室温液体中繁殖，18株中观察到3株球形体Hp在室温下的返祖，除常见球形变外的Hp杆状弯曲、长杆状、短杆状、长丝体、巨球体、丝球体、原生质体及细小"S"和弧形变异。

Besides coccoid of Hp transformations, curve-bacilliform, long-bacilliform, short bacilliform, long threadlike, giant coccoid, glomerule, trophoplast, and very small "s" or "c" shaped Hp were found.

并根据Hp形态变化的顺序及表型，初步发现Hp由典型"S"或多螺旋形态变为杆状弯曲、长杆状、短杆状、然后变为球形、长丝体、巨球体、丝球体、原生质体的规律。

To develop a rapid and accurate method for baculovirus titration, flow cytometry was adopted to quantitate baculovirus directly after staining with SYBR Green I.

为了快速而准确的测定杆状病毒的滴度，采用流式细胞术对经SYBR Green I染色后的杆状病毒直接计数。

Any of various bacteria, especially a rod-shaped bacterium .

杆状细菌一种细菌，尤指杆状细菌

Besides coccoid of Hp transformations, curve-bacilliform, long-bacilliform, short bacilliform, long threadlike, giant coccoid, glomerule, trophoplast, and very small "s" or "c" shaped Hp were found.

并根据Hp形态变化的顺序及表型，初步发现Hp由典型&S&或多螺旋形态变为杆状弯曲、长杆状、短杆状、然后变为球形、长丝体、巨球体、丝球体、原生质体的规律。

We have developed a method for detection of Hypodermal and hematopoietic necrosis baculovirus by polymerase chain reaction.

皮下和造血器官坏死杆状病毒［1］、对虾杆状DNA病毒［2］和白斑杆状病毒［3］是近年来引起全球对虾暴发性死亡的病原。

The homologues of ptp-1 and ptp-2 genes were found in some lepidopterous baculoviruses, but not in non-lepidopterous baculoviruses. ApNPV was one of the four baculoviruses contained both ptp-1 and ptp-2 genes.

只有部分鳞翅目杆状病毒编码ptp-1、ptp-2基因同源区，非鳞翅目杆状病毒不编码ptp-1、ptp-2基因同源区，而ApNPV为鳞翅目杆状病毒，同时编码ptp-1、ptp-2基因同源区。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

He is the most remarkable man that I have ever known.

他是我知道的人中最值得注意的人。

Increased appearance of lymphocele was noticed in patients with diabetic nephropathy, congenital malformations of the urinary tract, and inflammatory diseases, including glomerulopathy and extraglomerular ones, after high-voltage radiotherapy and after removal of the renal graft.

经过高压放射疗法和切除移植肾后，在糖尿病肾病患者、泌尿系统先天性畸形患者和炎症患者身上发现淋巴囊肿有所增加，包括肾小球病和球外疾病等。