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To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性,我们利用原位杂交和免疫组化等方法,研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动,结果表明:(1)热对各阶段的雄性生殖细胞都有损害,粗线期的初级精母细胞尤为敏感,实验性隐睾内的精原细胞尚未完全受到"致命"影响,说明精原细胞对热有一定的耐受性,但即使成为粗线期/双线期初级精母细胞,却未能获得由有丝分裂过渡到减数分裂的能力,呈现不同程度的凋亡,所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞,更谈不上长形精子细胞和精子的形成;(2)腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录,说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的;(3)正常兔睾丸组织中,〓在精原细胞和粗线期/双线期精母细胞中均有表达:cyclin B1蛋白在减数分裂前期的粗线期/双线期初级精母细胞中的表达量增加,于变态末期的精子细胞中达高峰。

There were the radicle polarity and the plumule polarity in differentiation of the first somatic embryogenesis.

此外,体细胞胚的发生和分裂是不同步的,往往在同一张切片上既可看到单个的胚性母细胞,又可见到二细胞、四细胞以致多细胞原胚并存的多样化原胚细胞群体。

The parameter of sphericity can be used to describe the similarity between centroblastic-centrocyte-centroblastic lymphoma cells and elips degree and regular form factor can describe the similarity between centroblastic-mantle-centroblastic lympoma cells.

圆球度可以用来描述中心母细胞--中心细胞--中心母细胞性淋巴瘤细胞之间的相似性;椭圆度、规化形状因子可以描述中心母细胞--套细胞--中心母细胞性淋巴瘤细胞之间的相似性。

A lion's mane is a secondary sex characteristic of males, and its size and darkness are believed to determine his attractiveness to the lionesses.

一只狮子的鬃毛是第二性征的壮丁,它的尺寸大小和暗度是认为决定他的吸引力对于母狮。

Results There was not specificity of clinical symptoms in liposarcoma of the orbit, the major clinical aspects were intraorbital occupied lesion and local hyperaemia,swell and pain which was similar to an acute inflammation. Four different subtypes of liposarcomas could be observed in orbit.In past literature,myxoid was the most common subtype(14 cases,70%),secondly subtype was well differentiated(3 case),round cell and pleomorphic subtype were rare(each 1 case).This case was well differentiated mixed component of round and pleomorphic lipoblasts.

结果:脂肪肉瘤临床表现为眶内占位和貌似急性炎症的局部红、肿、痛,无特征性的症状;病理分型4型,文献中报道以粘液样型最多见(14例,占70%);次为分化良好型,3例;圆形细胞为主型及多型性各1例;分型不详2例;本例为分化良好型为主伴有圆形脂母细胞及多型性脂母细胞成分。

Myoid/myofibroblastic areas in DFSP possibly represents the hyperplasia of stromal myofibroblasts, rather than true myofibroblastic differentiation of the neoplastic cells.

DFSP中的肌样/肌纤维母细胞性分化可能是肿瘤间质中肌纤维母细胞增生的结果,并非代表了瘤细胞的真性肌纤维母细胞性分化。

The results showed that the expression of calm3 and trm112l genes had no remarkable differences in oocytes and 8-cells stage embryos, but were significantly different in blastocysts. It was supposed that these two genes were possibly maternal genes, and they might be involved in oocyte maturation and zygotic genome activation.

结果显示,在卵母细胞和8细胞期胚胎,基因calm3和trm112l的表达量无显著性差异,而在囊胚期的表达量与前两个时期的表达量存在显著性差异,推测这两个基因在牛胚胎发育过程中的表达可能属于母型调控,提示该基因在卵母细胞成熟和合子基因激活中起一定作用。

Foals are born to a mare, a sexually mature female.

小马驹是枣红色的性成熟的母马产下的。

The marrow between the pink bone trabeculae seen here is nearly 100% cellular, and it consists of leukemic cells of acute lymphocytic leukemia that have virtually replaced or suppressed normal hematopoiesis .

如图可见粉红色的骨小梁之间的骨髓几乎100%是细胞,是由急性淋巴母细胞性白血病的白血病细胞构成,这些细胞实际上替代或抑制了正常的造血作用。

Elemental and isotopic data suggest that the chemical variation of the mid-Neoproterozoic rift-related basic lavas in South China cannot be explained by crystallization from a common parental magma.

元素和同位素数据表明,华南新元古代中期裂谷基性熔岩的化学变化不是由一个共同的母岩浆结晶分异作用所产生。

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I will endeavour to find you some assistance.

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起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像,后来回台湾,甚至去年在北京,居然也四处看见她的海报,才惊讶她的魅力之大。

Ah may dee:You are chinese living in a democratic country.

你是居住在民主国家的中国人吧。