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Adenovirus 5第二军医大学博士学位论文英文摘要胸心外科专业(WAd5) and PDC-MLC-VEGF were transferred to the adenoviral packaging cell HEK 293cell by lipofectamine 2000 mediated gene transfer method to pack the virus; The desired Advectors were purified by density gradient ultracentrifuge and titrated in 293 cells afteridentified.

Ad.mlc-hVEGF165 体外转染心肌细胞的实验研究分离培养乳鼠心肌细胞,在同一 MOI 值下,Ad.mlc-hVEGF165 和 Ad.hVEGF165分别转染心肌细胞,以 RT-PCR 法和 Western 印迹分析法分别检测转染后心肌细胞内VEGF mRNA 的转录及 VEGF 蛋白的表达,以 ELISA 法检测转染后心肌细胞分泌的VEGF 蛋白质,MTT 等方法观察其对促内皮细胞增殖作用。

In semiology, epigastric fullness is very similar to GDS.

从症状学上看,心下痞与现代医学的GDS有非常密切的关系。

Observe the effect of Fuling Gancao Decoction on gastric liquid emptying and content of nitric oxide and acetylcholine esteras in the gastric fundus,antrum and duodenal tissu of functional dyspepsia rats and investigate the mechanism.1 Reference reviewThe reference review discussed the source of disease name of TCM for FD,etiology and pathogenesis of TCM,selection of therapeutic methods by different stages or according to the differential syndrome diagnosis,advance on the clinical and experimental research as well as the problems lying in the research of TCM and corresponding strategies.We also have summarized the recent research progresses on the FD.In the meanwhile,we further exploited the pathologic characteristics,diagnosis and treatment of FD;summarized etiology and pathogenesis of TCM for FD and application of Classical Prescriptions in clinical and experimental study;Collected and arranged the Literatures and summarized the recent research progresses of Fuling Gancao Decoction;Finally, collected and arranged the literatures of proved cases and clinical experiences of Fuling Gancao Decoction.2 Theoretical studyThe Theoretical study first discussed the source of TCM for stomach deficiency and congestion of fluid-retention in syndromes of FD;Secondly, summarized the indications of Fuling Gancao Decoction,that is:①epigastric throb,②cold hands and feet in syncope,③hydroadipsia,④gastric blockade,⑤gastric splashing sound,⑥no desire for drinks,⑦light white tongue,thin-white and smooth fur,⑧deep and wiry pulse are the indications of Fuling Gancao Decoction;Subsequently,analyzed the composition of Fuling Gancao Decoction,considered that ginger is the main drug which treating congestion of fluid-retention in Middle-Jiao by Zhang Zhongjing.3 Experimental study3.1 Experiment oneObjective:To establish the animal model of FD with delayed gastric liquid emptying and evaluat it.

同时观察茯苓甘草汤对FD大鼠胃液体排空及胃底、胃窦及十二指肠中乙酰胆碱酯酶、一氧化氮含量的影响,探讨茯苓甘草汤的作用机制。1文献综述参照古、今中医有关文献对FD的中医病名、病因病机、辨证论治方法、临床及实验研究进展进行了探讨,并对现代医学在FD研究中所取得的(来源:A0bBC论文网www.abclunwen.com)进展进行了总结,进一步明确了FD的发病机制、诊断和治疗的研究进展;总结了中医对FD的病因及发病机制、中医诊断及经方治疗FD临床及实验研究进展;并搜集和整理已发表的有关茯苓甘草汤研究报道,阐述了茯苓甘草汤的现代研究进展;最后搜集和整理了近现代著名医家应用茯苓甘草汤的经验及验案。2理论研究理论研究部分首先对功能性消化不良胃虚饮停证的认识进行了理论溯源;其次对茯苓甘草汤的适宜证象进行总结,认为:①心下悸,②四肢不温,③口不渴,④胃中痞满,⑤胃中振水音,⑥不欲饮水,⑦舌淡白,苔薄白而滑,⑧脉沉弦是茯苓甘草汤的主证;随后对茯苓甘草汤方药组成解析,认为生姜是仲景治疗中焦饮停的一味主药。3实验研究3.1实验一目的:探讨建立功能性消化不良胃虚饮停证的动物模型方法并对其进行评价。

So when he saw this man sorely wounded, and yet still capable of despatching several dastardly salt-smugglers single-handed, he felt as if he'd finally come face to face with one of his idols.

他听书听得多了,对故事中英雄好汉极是心醉,眼见此人重伤之余,仍能连伤不少盐枭头目,心下仰慕,书中英雄常说的语句便即脱口而出。

First, the angular velocity function and maneuver load for certain maneuver flight (including hover, dive-hike and these two combined) are set up. The simulation results of the vibration of double-disk rotor system with cantilever supported on rigid bearings show that rotor would be apparently deformed under maneuver load. Second, the performance of oil force of SFD in maneuver flight is analyzed. The results show that in maneuver flight, the SFD with centering spring will become eccentric and its oil force will be time-related and offset-related.

首先,建立了水平盘旋、俯冲拉起及盘旋+俯冲拉起复合机动三种机动飞行条件下的机动角速度函数,并确定了对应机动飞行条件下的机动附加载荷(附加离心力和附加陀螺力矩),分析了带刚支的双盘悬臂转子系统在机动飞行条件下的振动特性,发现发动机转子在机动飞行时会发生明显变形;随后,分析了非定心挤压油膜阻尼器的油膜力特性,机动飞行时,油膜轴颈在机动附加载荷的作用下偏离外环中心,使得带定心弹支的挤压油膜阻尼器也变成了非定心挤压油膜阻尼器,其非定心挤压油膜力是瞬态时变的,并且与SFD油膜轴颈偏移量和进动形态有关;然后,在经典的Jeffcott转子系统中引入带定心弹支的挤压油膜阻尼器,分析了机动飞行时非线性转子系统的振动特性,发现挤压油膜阻尼器油膜力的非协调特性也反映到转子系统的振动响应中;之后,对机动飞行条件下带挤压油膜阻尼器的双盘悬臂转子系统的振动特性进行了分析,并重点分析、总结了俯冲拉起机动飞行时,系统结构参数和工作状态参数(转速比、机动飞行半径、机动角速度等)对该系统振动特性的影响。

First, the angular velocity function and maneuver load for certain maneuver flight (including hover, dive-hike and these two combined) are set up. The simulation results of the vibration of double-disk rotor system with cantilever supported on rigid bearings show that rotor would be apparently deformed under maneuver load. Second, the performance of oil force of SFD in maneuver flight is analyzed. The results show that in maneuver flight, the SFD with centering spring will become eccentric and its oil force will be time-related and offset-related. Then, SFDs are introduced into typical Jeffcott rotor system. The whole system will be statically displaced in maneuver flight and the nonsynchronous components of oil force appear in the response of rotor system.

首先,建立了水平盘旋、俯冲拉起及盘旋+俯冲拉起复合机动三种机动飞行条件下的机动角速度函数,并确定了对应机动飞行条件下的机动附加载荷(附加离心力和附加陀螺力矩),分析了带刚支的双盘悬臂转子系统在机动飞行条件下的振动特性,发现发动机转子在机动飞行时会发生明显变形;随后,分析了非定心挤压油膜阻尼器的油膜力特性,机动飞行时,油膜轴颈在机动附加载荷的作用下偏离外环中心,使得带定心弹支的挤压油膜阻尼器也变成了非定心挤压油膜阻尼器,其非定心挤压油膜力是瞬态时变的,并且与SFD油膜轴颈偏移量和进动形态有关;然后,在经典的Jeffcott转子系统中引入带定心弹支的挤压油膜阻尼器,分析了机动飞行时非线性转子系统的振动特性,发现挤压油膜阻尼器油膜力的非协调特性也反映到转子系统的振动响应中;之后,对机动飞行条件下带挤压油膜阻尼器的双盘悬臂转子系统的振动特性进行了分析,并重点分析、总结了俯冲拉起机动飞行时,系统结构参数和工作状态参数(转速比、机动飞行半径、机动角速度等)对该系统振动特性的影响。

Attentive tenderness凝聚于心的那份温存Scene Four: Man joins the party with the comrades, binged one cup after another and got drunk out of expectation.

场景四:男人在外和多年不见的战友聚会,觥杯交错间海吃山喝,一不小心烂醉如泥,回家后吐的一塌糊涂还满口胡言乱语,女人没有唠叨,更没有动怒,吃力地把男人拖到床边放好,细心的为他换上睡衣,又急忙彻好醒酒汤让他慢慢喝下。

At this moment all was bright, clear and clam in my heart.

这时心下光明澄静,如登仙界(7),如归故乡。

Rhizoma alismatis and atractylodes were used together for the first time in Zexie liquid of the Internal Classic of Emperor, which is used to treat vertigo due to retention of damp-turbid substance, consisting of rhizoma alismatis, atractylodes and pyrola.

泽泻和白术配合使用最早见于《内经》的十三方中的&泽泻饮&,方由泽泻、白术、鹿衔草三味药组成,用于治疗湿浊中阻之眩晕,仲师将前方减去鹿衔草,新创金匮泽泻汤用于治疗心下支饮,其人眩冒。

Compared with the other three, whom, if appearing in a hospital, might have been sent to the women's delivery room asap.

心下暗自怀疑老公是有意让我来看,和其他几个男人相比,他的身材还算正常。

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He knows that he's one of the mostdominant players in the NBA right now.

我知道他现在是联盟里最有统治力的球员之一了。

Using analysis method of BCG , the dissertation analyzed the management of voluntary service organizations .

利用BCG分析方法对我国的志愿服务组织管理进行了分析。

A caret is a mark place below a line to show where something should be put in .

脱字符是加在一行文字下面的符号,表示此处应加入一些东西。