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Methods The Ames test, micronucleus test and comet assay were used to detect the genotoxicity of bactericide flutriafol.

应用Ames试验、小鼠骨髓嗜多染红细胞微核试验、慧星试验研究粉唑醇的遗传毒性。

PCR reactions were carried out on ABI9600 thermal cycler and the products of PCR were analyzed by vertical non-denaturing polyacrylamide gel electrophoresis.

利用引物设计软件分别对法医常见昆虫的常染色体微卫星区域和线粒体DNA进行引物设计,使用PCR技术分别对其进行扩增,用聚丙烯酰胺电泳对PCR产物分离、银染。

The endermic LD50 to rat and mouse were 926 mg/Kg and 1210 mg/Kg respectively. The results of subscute toxicity test in rats showed that the content of blood urea nitrogen in exposure groups was higher than that in control group, the histopathological changes of testis observed in exposure groups were obvious. Mutagenic tests including bacillus subtilis DNA rec-assay, micronucleus ...

其大、小鼠经皮LD_(50)分别为926mg/kg和1210mg/kg;大鼠90天经皮染毒,试验组BUN含量高于对照组,光镜下,试验组睾丸的组织学结构有明显改变;枯草杆菌DNA重组试验、小鼠骨髓多染红细胞微核试验和小鼠睾丸染色体畸变分析试验均获得阳性结果。

MATERIALS AND METHODS: The health-preserving cigarettes were made by certain Chinese herbal medicine such as piper sarmentosum Roxb. We made these cigarettes into a smoke liquid of normal saline solution as an experimental material to do an acute toxicity test in mice. A micronucleus test of mice bone marrow polychromatophilic erythroblast and an Ames test were performed.

材料与方法:在香烟中加入中草药假药等制成中草药保健香烟,用该香烟制得生理盐水烟雾液为实验材料,进行小鼠急性毒性实验、小鼠骨髓嗜多染红细胞微核试验和Ames试验。

MATERIALS AND METHODS: The root tips of Vicia faba were treated with different concentrations of sodium sulfite and sodium nitrite (in the concentrations of 25;50;100;200;400 mg/L) with distilled water as control for four hours.The root tips of Vicia faba were stained with carbol fuchsin and examined. The micronucleus rate and the rate of chromosome aberration were determined within root tip cells of Vicia faba.

材料与方法:用不同浓度的亚硫酸钠和亚硝酸钠(25、50、100、 200、400 mg/L)分别处理蚕豆根尖4 h,并以蒸馏水作为对照,用石炭酸品红染液染色,分别观察、测定蚕豆根尖细胞的微核率和染色体畸变率。

Use the hair small to furl gently, ten minutes small after ironing, can appear rise and fall not big curvature, if add a few to carry,catch, more show amorous feelings 10 thousand kinds.

把头发用小卷轻轻卷起,十几分钟的微烫之后,就会出现起伏不大的曲度,如果加几缕挑染,就更显风情万种。

The test results of micronucleus and chromosome aberration in mice were all negative.

小鼠骨髓多染红细胞微核试验和小鼠睾丸精母细胞染色体畸变试验均为阴性。

Statistical analysis was done for the rate of bone marrow cell micronucleus induced by different dose of formaldehyde.

对不同浓度气态甲醛诱导昆明种小鼠骨髓嗜多染红细胞微核率进行了统计分析。

Methods Micronucleus test in mouse bone marrow cells and chromosomal aberration test in human peripheral blood lymphocytes.

方法小鼠骨髓嗜染红细胞微核试验和人外周血淋巴细胞染色体畸变试验。

Our research work investigated the possibility of applying the vaccine to gene therapy of CEA positive tumors and set the foundation for further study of its clinical application.

为解决上述问题、提高基因治疗的安全性,本研究采用细胞移植免疫用隔离工具-微胶囊包裹转染部分CEA基因的人成纤维样骨髓基质细胞HFCL,制备出CEA微囊化转基因细胞疫苗,并进行了疫苗在小鼠体内的表达及其免疫学性质的研究,探讨该疫苗应用于CEA阳性肿瘤治疗的可能性,并为进一步的临床应用研究奠定了基础。

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