奥索尔诺

Antigene peptide nucleic acid specifically inhibits MYCN expression in human neuroblastoma cells leading to cell growth inhibition and apoptosis Roberto Tonelli,Stefania Purgato,Consuelo Camerin,Raffaele Fronza,Fabrizio Bologna,Simone Alboresi,Monica Franzoni,Roberto Corradini,Stefano Sforza,Andrea Faccini,Jason M. Shohet,Rosangela Marchelli,and Andrea Pession Department of Pediatrics, University of Bologna,Sant'Orsola-Malpighi Hospital, Bologna, Italy; Department of Organic and Industrial Chemistry, University of Parma, Parma,Italy; and 3Center for Cell and Gene Therapy, Texas Children's Cancer Center, Baylor College of Medicine, Houston, Texas Abstract We developed an antigene peptide nucleic acid for selective inhibition of MYCN transcription in neuroblastoma cells, targeted against a unique sequence in the antisense DNA strand of exon 2 of MYCN and linked at its NH2 terminus to a nuclear localization signal peptide.

抗原肽核酸抑制MYCN具体表达的神经母细胞瘤细胞，导致细胞生长的抑制和凋亡罗伯托利，斯特凡Purgato ，苏埃洛卡默琳，拉菲尔Fronza ，梅奥尼博洛尼亚，西蒙娜Alboresi ，莫妮卡弗兰索尼，罗伯托Corradini ，斯特凡诺斯福尔扎，安德烈Faccini ，杰森先生Shohet ， Rosangela Marchelli ，和安德烈Pession儿科，博洛尼亚大学，圣' Orsola -马尔皮基医院，博洛尼亚，意大利；有机部和工业大学化学帕尔马，帕尔马，意大利和3Center的细胞和基因治疗，德州儿童癌症中心， Baylor医学院，得克萨斯州休斯敦摘要我们开发了一种抗原肽核酸的选择性抑制神经母细胞瘤MYCN转录细胞，针对一个独特的序列反义DNA链的第2外显子，并与在MYCN 其氨基端为核定位信号肽。

Patrick s purgatory , the salmon leap , maynooth college refectory , curley s hole , the three birthplaces of the first duke of wellington , the rock of cashel , the bog of allen , the henry street warehouse , fingal s cave - all these moving scenes are still there for us today rendered more beautiful still by the waters of sorrow which have passed over them and by the rich incrustations of time

二湖谷，基拉尼那些可爱的湖泊，克朗麦克诺伊斯487的废墟，康大寺院，衣纳格峡谷和十二山丘，爱尔兰之眼488 ，塔拉特的绿色丘陵，克罗阿帕特里克山489 ，阿瑟吉尼斯父子股份有限公司的酿酒厂，拉夫尼格湖畔，奥沃卡峡谷490 ，伊索德塔，玛帕斯方尖塔491 ，圣帕特里克邓恩爵士医院492 ，克利尔岬角，阿赫尔罗峡谷493 ，林奇城堡，苏格兰屋，拉夫林斯顿的拉思唐联合贫民习艺所494 ，图拉莫尔监狱，卡斯尔克尼尔瀑布， 495市镇树林约翰之子教堂496 ，莫纳斯特尔勃衣斯的十字架，朱里饭店，圣帕特里克的炼狱， 497鲑鱼飞跃，梅努斯学院饭厅，柯利洞穴， 498第一任威灵顿公爵的三个诞生地，卡舍尔岩石， 499艾伦沼泽，亨利街批发庄，芬戈尔洞500所有这一切动人的501情景今天依然为我们而存在。

The battle once begun, its very various changes,--the resistance of Hougomont; the tenacity of La Haie-Sainte; the killing of Bauduin; the disabling of Foy; the unexpected wall against which Soye's brigade was shattered; Guilleminot's fatal heedlessness when he had neither petard nor powder sacks; the miring of the batteries; the fifteen unescorted pieces overwhelmed in a hollow way by Uxbridge; the small effect of the bombs falling in the English lines, and there embedding themselves in the rain-soaked soil, and only succeeding in producing volcanoes of mud, so that the canister was turned into a splash; the uselessness of Pire's demonstration on Braine-l'Alleud; all that cavalry, fifteen squadrons, almost exterminated; the right wing of the English badly alarmed, the left wing badly cut into; Ney's strange mistake in massing, instead of echelonning the four divisions of the first corps; men delivered over to grape-shot, arranged in ranks twenty-seven deep and with a frontage of two hundred; the frightful holes made in these masses by the cannon-balls; attacking columns disorganized; the side-battery suddenly unmasked on their flank; Bourgeois, Donzelot, and Durutte compromised; Quiot repulsed; Lieutenant Vieux, that Hercules graduated at the Polytechnic School, wounded at the moment when he was beating in with an axe the door of La Haie-Sainte under the downright fire of the English barricade which barred the angle of the road from Genappe to Brussels; Marcognet's division caught between the infantry and the cavalry, shot down at the very muzzle of the guns amid the grain by Best and Pack, put to the sword by Ponsonby; his battery of seven pieces spiked; the Prince of Saxe-Weimar holding and guarding, in spite of the Comte d'Erlon, both Frischemont and Smohain; the flag of the 105th taken, the flag of the 45th captured; that black Prussian hussar stopped by runners of the flying column of three hundred light cavalry on the scout between Wavre and Plancenoit; the alarming things that had been said by prisoners; Grouchy's delay; fifteen hundred men killed in the orchard of Hougomont in less than an hour; eighteen hundred men overthrown in a still shorter time about La Haie-Sainte,--all these stormy incidents passing like the clouds of battle before Napoleon, had hardly troubled his gaze and had not overshadowed that face of imperial certainty.

交锋以后，战争的非常复杂惊险的变化，乌古蒙的阻力，圣拉埃的顽抗，博丹的阵亡，富瓦战斗能力的丧失，使索亚旅部受到创伤的那道意外的墙，无弹无药的吉埃米诺的那种见死不退的顽强，炮队的陷入泥淖，被阿克斯布里吉击溃在一条凹路里的那十五尊无人护卫的炮，炸弹落入英军防线效果不大，土被雨水浸透了，炸弹陷入，只能喷出一些泥土，以致开花弹全变成了烂泥泡，比雷在布兰拉勒出击无功，十五营骑兵几乎全部覆没，英军右翼应战的镇静，左翼防守的周密，内伊不把第一军的四师人散开，反把他们聚拢的那种奇怪的误会，每排二百人，前后连接二十七排，许多那样的队形齐头并进去和开花弹对抗，炮弹对那些密集队伍的骇人的射击，失去连络的先锋队，从侧面进攻的炮队突然受到拦腰的袭击，布尔热瓦、东泽洛和迪吕特被围困，吉奥被击退，来自综合工科学校的大力士维安中尉，冒着英军防守热纳普到布鲁塞尔那条路转角处的炮火，在抡起板斧去砍圣拉埃大门时受了伤，马科涅师被困在步兵和骑兵的夹击中，在麦田里受到了贝司特和派克的劈面射击和庞森比的砍斫，他炮队的七尊炮的火眼全被钉塞，戴尔隆伯爵夺不下萨克森－魏玛亲王防守的弗里谢蒙和斯莫安，第一○五联队的军旗被夺，第四十五联队的军旗被夺，那个普鲁士黑轻骑军士被三百名在瓦弗和普朗尚努瓦一带策应的狙击队所获，那俘虏所说的种种悚听的危言，格鲁希的迟迟不来，一下便倒在圣拉埃周围的那一千八百人，比在乌古蒙果园中不到一个钟头便被杀尽的那一千五百人死得更快，凡此种种迅雷疾风似的意外，有如阵阵战云，在拿破仑的眼前掠过，几乎不曾扰乱他的视线，他那副极度自信的龙颜，绝不因这些变幻而稍露忧色。

In addition to Rostropovich, with whom Mr. Numajiri first performed during the New Japan Philharmonic's Shostakovich Festival, he has collaborated with other such artists as Anne-Sophie Mutter, Frank Peter Zimmermann, Zoltan Kocsis, Bruno-Leonardo Gelber, Jean-Philippe Collard, Michel Béroff, Cyprien Katsaris, Tzimon Barto, Karl Leister and Rune Kollo.

此外，他还与罗斯特罗波维奇在新日本爱乐乐团的肖斯塔科维奇音乐节同台演出，与他合作的艺术家还包括安娜-索菲·穆特、弗朗克·彼得·齐莫尔曼、佐尔坦·科西斯、布鲁诺-莱奥纳多·盖尔伯、让-菲利普科拉尔、米歇尔·贝罗夫、塞普林·加查理斯、齐蒙·巴尔托、卡尔·莱斯特尔以及鲁内·科洛。

The French Provence is a peaceful and warmhearted place, its colors of natural environment is variation and full-bodied, it has conceived numerous great artistes: from Van Gogh, Picasso, to Pagnol and Renoir. All of which explained the importance of ethereally environment to accomplish the unprecedented works.

法国的普罗旺斯安宁而温馨，自然环境色彩变化丰富又浓郁，孕育了无数伟大的艺术家：从梵高Van Gogh、毕加索Picasso，以至帕尼奥尔Pagnol及雷诺阿Renoir，都说明了幽雅环境对打造惊世作品的重要性。

Sent to Florence at the age of sixteen by Cardinal Silvio Passerini, he joined the circle of Andrea del Sarto and his pupils Rosso Fiorentino and Jacopo Pontormo where his humanist education was encouraged.

发送到佛罗伦萨在16岁以下的枢机西尔维奥帕塞里尼，他加入了循环安德烈删除Sarto和他的学生罗索伦蒂诺和雅格布蓬托尔莫他的人文教育是鼓励。

I think he's learned how to approach hitters....

Johhny Damon说，『他已是最好的了，再没有人能够比他做的更好，我想他的球技已经完全成熟了，而且他也已经学会了如何去对付和压迫各种打击者。。。。。。

In effect, an 18 foot King Cobra could actually look down onto the average human being.

际上，一个18尺眼镜蛇王其实可以俯视到的平均人。