增殖

The third passage chondrocytes were divided into blank group, different desity PAP groups, different desity glucosaminsalfate groups which were passaged to 4th generation and contrast to the 2nd passage group. The chondrocytes of different groups were detected with the method of histochemistry for S-A-β-gal,and with alcian blue test for the content and constructure of GAG of ECM, immuocytochemistry for type Ⅱcollagen and PCNA, MTT assay for proliferation, RT-PCR for type Ⅱcollagen and Aggrecan, flow cytometry for cell life cycle and proliferation index,by which to observe PAP's function regarding to the appearance and functional status in the process of chondrocyte's cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

The 3rdpassage chondrocytes were divided into blank group, different concentration PAP groups,different concentration glucosaminsalfate groups and were sequently passaged to 4thgeneration. The 2nd passage chondrocytes was contrasted as young cells group. Thechondrocytes of different groups were detected with the methods of histochemistry forS-A-β-gal, and with alcian blue test for the content and constructure of GAG of ECM,immuocytochemistry for typeⅡcollagen and PCNA, MTT assay for proliferation, RT-PCRfor typeⅡcollagen and Aggrecan, flow cytometry for cell life cycle and proliferationindex,by which to observe PAP"s function regarding to the appearance and functional status inthe process of chondrocyte"s cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

Result: Rhesus BMSC can be got in mass by density gradient centrifugation.; Rhesus BMSC can adhere to PLGA fiber quickly after plant and its growth , division ,proliferration is good; Some cells arrange like rosary on PLGA fiber surface, some cells still form the catenate or flaky cell bridge joined among PLGA fiber.

结果：采用密度梯度离心的方法可得到大量增殖能力强的猕猴骨髓基质干细胞；接种后的骨髓基质干细胞很快粘附于PLGA纤维上，生长、分裂、增殖良好，在PLGA纤维表面形成串珠样排列，部分细胞还形成连接在PLGA纤维间的链状或片状细胞桥。

The theory is cell kinetics and mechanism of drugs.

主要是根据细胞动力学原理和药物的作用机理分析，如果把药物集中应用，一方面只是一过性的杀灭增殖期的白血病细胞，对静止期细胞影响不大，另一方面对正常细胞造成较大损伤，而如果药物作用于几个细胞增殖周期，对白血病细胞进行几个循环的打击，会清除更多白血病细胞，而对正常细胞损伤较小。

With cellobiose and cellooligosaccharide as carbon source, the proliferation multiple of B. odolescentis was 2.14 and 2.84, respectively.

以纤维二糖、纤维低聚糖为C源增殖青春双歧杆菌，菌体质量浓度增殖倍数分别为2.14、2.84。

Tumor associated protein N35 was located on the centriole of the chromosomal domain at the S-G2 phage.Conclusions The tumor associated protein N35 might be a tumor cell growth regulator, expressed only it's the cancer cells and related with the proliferation of cancer cells. It is one of tumor markers.

肿瘤相关蛋白N35可能是一种只存在于肿瘤细胞并与其增殖活动密切相关的重要的肿瘤细胞生长调节蛋白，其功能可能与肿瘤细胞无限制增殖活动有关，是肿瘤标志物之一。

The PKC family is subdivided into three groups; the classical PKC members are Ca〓 and diacylglycerol dependent, the novel PKCn are Ca〓-independent but diacylglycerol dependent and the atypical PKCs are not activated by Ca〓and diacylglycerol in vitro.

丝裂素活化蛋白激酶是一族在脊椎类动物体内广泛存在的蛋白质丝氨酸/苏氨酸激酶，是目前发现的最主要的一个生长信号调节蛋白，是细胞内多种促细胞增殖信息传递的共同通路或称会聚点，是细胞表型变化和增殖的蛋白激酶。

To investigate the expression of COX-2 in condyloma acuminatum and its relationship with the proliferation in tissues.

目的探讨环氧化酶-2(COX-2)在尖锐湿疣组织中的表达及与细胞增殖和血管增殖的关系。

The result show in transplanted vessel EF-1 and PCNA are mainly expressed in smooth muscle cells and Clemons rate proliferation of SMC is the core of endothelium proliferation.In the third part of our experiment we use fluid nitrogen to fire transplanted rein, crofter tissue homogeneous to test the expression of CE and EF-1 gene we use RT-Craned immunohistologiccal method to test mKNA and protein level respectively and analyze the expression of these tm genes in transplanted vein.

本实验的第二部分，我们将术后大鼠分为10组，每组8只，分别于术后6hours、24hours、72hours、1W、10days、2W、3W、4W、6W、8W切取移植静脉，将切取的不同时期的移植静脉分别置于10％中性福尔马林溶液中固定和液氮冷冻固定，将福尔马林溶液固定的标本制成4μm厚石腊切片，，常规进行病理观察大体改变，静脉标本HE染色后于光镜下观内膜增殖情况，并应用PCNA单克隆抗体，观察SMC增殖情况。

Administering adequate but not excessive solution of crystalloid to preserve blood pressure during the recipient surgery.

在反映粘膜上皮细胞增殖程度的DNA含量相对倍体值和细胞增殖核心抗原方面，实验组也明显优于对照组。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。