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The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling . Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied.

在研究过程中,采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术,系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化,T和B淋巴细胞、T淋巴细胞亚群的动态变化规律,淋巴细胞表面某些粘附分子的变化,淋巴细胞和神经元的坏死与凋亡,雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律,脑组织中围官性细胞浸润的细胞类型。

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling. Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied. The key research results were.(1)The average percentage of CD19+cell in blood, bursa and spleen, after 3, 5 days of inoculation, was significantly Phybridization revealed that the number of IL-8 and MIP-1βproducing cells was increased in the infected brain.

在研究过程中,采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术,系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化,T和B淋巴细胞、T淋巴细胞亚群的动态变化规律,淋巴细胞表面某些粘附分子的变化,淋巴细胞和神经元的坏死与凋亡,雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律,脑组织中围官性细胞浸润的细胞类型。

In this paper, I establish a set of updating the basic land-use map technology and flow after I go deep into studying the methods of updating the basic land-use map based on 3S technology. It is very important point that I bring forward some methods of change information automatic detected founding on land-use map unit, cross correlation analysis with multispectral image and object-oriented classification aiming at difference data and difficult identifying change information problems.

本文在深入研究了基于3S技术更新土地利用图件的方法基础上,从实用化的角度出发,建立了一套利用3S技术更新土地利用基础图件的技术流程与方法,并针对不同的数据情况和变化信息自动提取等问题提出了基于土地利用图斑单元的知识库变化信息自动发现方法、多波段遥感影像交叉相关分析变化识别方法及面向对象的遥感影像分类变化检测方法。

Except crossbred buffalo, the percentage of amino acids of milk from buffalo of different breeds and generations are close.

各品代水牛奶的密度平均值变化范围为1.028~1.032;酸度平均值变化范围是15.36-19.82 T;pH值的平均值变化范围从6.46—6.88;黏度平均值的变化范围由2.44-3.11Pa·S。

The shape of chloroplast didn't change after seedling was treated by GA〓, but the number of lamllasomes increased, and starch grain enhanced. These changes were accord to that of the rate of photosynthesis. Mitochondria varied greatly, the structure of mitochondria could see vaguely and carinace almost deliquesce after 4days. Enrolucrum emerged until the seventh day after treatment and endomembrance of mitochondria recovered gradually, cristae could be seen. On the other hand, the endomembrance of mitochondria in CK (normal non-bolting) was obvious and the quantity of cristae in the mitochondria was much more than the GA〓 treatment. This showed that respiration might decrease after GA〓 treatment while reduced the depletion of photosynthesis products and enhanced bolting.

GA〓处理后抽苔过程中叶绿体形状未发生变化,但类囊体增多,淀粉粒增多,这种变化与光合速率的变化吻合;线粒体变化较大,处理后4天线粒体结构较模糊,嵴不清晰,7天时外膜形成,内膜也逐渐恢复,能见到嵴,而对照线粒体内膜结构明显,嵴清晰数量也多,说明GA〓处理后,呼吸作用降低,减少光和产物的消耗;细胞膜无破损,较光滑完整,说明对细胞膜没有伤害。

The economic growth accounting of China's provinces between 1990 and 2004 finds some results like the following: no matter whether human capital was introduced or not, there were technological inefficiencies in most provinces'economic activity which were pure technological inefficiencies or scale technological inefficiencies; technological change, capital deepening and human capital accumulating all promoted labor productivity, but technological efficiency change played a negative role during these years as a whole; the descending order of provincial difference of labor productivities'changes brought out by each factor was technological change, capital deepening, technological efficiency change and human capital accumulating; each province's pure technological efficiency change or scale efficiency change not only had different size, but also different evolution route.

中国各省在1990~2004年间的经济增长核算结果表明:不论是否引入人力资本,大多数省份的经济活动都存在着技术无效率,这既包括纯技术无效率,也包括规模无效率;技术进步、资本深化和人力资本积累都促进了劳动生产率的提高,而技术效率的变化整体上来说在其中起阻碍作用;各因子引致的劳动生产率变化的省际差异从大到小依次为技术进步、资本深化、技术效率的变化和人力资本积累;各省的纯技术效率或规模效率的变化不仅大小不同,而且它们的演变路径也不一样。

Now do we must discuss are not need to change,Should study,How but is you should quickly study,More accurate diastrophism,Only was invariable possibly is changes,Moreover or fast \ initiative diastrophism,Draws a inappropriate analogy,In the past everybody said ten years rub a sword, today we perhaps still needed for ten years to rub a sword,What but is different is we while rubs a sword also to want each hour to make to beat a dog the stick Otherwise, your sword does not have 磨出 to come, the neck on is wiped by yours enemy,Otherwise, the sword 磨出 has come also is the toy The competition is the war,I thought this selected already no need to doubt,The present age, the plagiarism and the surmounting speed nearly already was the speed of light, the people do not even have the time calmly to get down the personal status own achievement.

请高手按照演讲的体裁来翻译:如今的年代我们所要讨论的不是需不需要变化,应不应该学习,而是你应该如何更快地学习,更准确地变化,唯一不变的可能就是变化了,而且还是快速地\主动地变化。打个不恰当的比方,过去大家都说十年磨一剑,今天我们也许仍然需要十年磨一剑,但是所不同的是我们在磨一剑的同时还要每小时制造一根打狗棒。否则,你的剑还没有磨出来,脖子就被你的敌人抹了,而且我们在磨剑的间隙里,要不断地\想方设法地了解一下我们磨剑的方法是否先进,否则,剑磨出来了也是玩具。竞争就是战争,我想这点已经没有什么需要置疑的了。

Compared with the previous, and now the physical hard disk have been some changes in the structure of a direct impact on the realization of the hard drive changes in the way instruction, the most important thing is to seek changes in the way the hard disk formatting result in changes in instruction.

与以前相比,现在硬盘的物理结构发生了一些变化,直接影响到相应的硬盘指令实现方式的变化,其中最重要的就是硬盘寻道方式的变化导致的格式化指令的变化

On the basis of the kinetic equation of substrate reaction in the presence of urea or guanidine hydrochloride, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present studies indicate that:①In the presence of urea or guanidine hydrochloride, enzyme-substrate complexes lose their activity less rapidly than the free enzyme. Therefore, both substrates, NADPH and 7, 8-dihydrofolate, protect dihydrofolate reductase against inactivation.②The denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process, so it may be an"all or none"process.③The GdnHCl-induced unfolding of the dihydrofolate shows a biphasic transition, while the change in the enzyme activity is a single exponential process. The rate constant of inactivation is consistent with that of the fast conformational change. Therefore, the kinetic intermediate of protein unfolding should be a partially folded and inactive form.

我们根据在脲或盐酸胍存在下的底物反应动力学方程求得游离酶和酶底物二元、三元复合物的微观动力学常数,结果表明:①酶-底物二元、三元复合物的失活速度明显慢于游离酶,说明两个底物二氢叶酸和NADPH对酶的失活都具有一定程度的保护作用;②在脲作用下,酶的失活和构象变化均为单指数项过程,而且酶的活力丧失和三级结构变化是同时发生的,说明二氢叶酸还原酶的脲变性可能是一个"全或无"的两态过程;③在盐酸胍作用下,酶的构象变化为两相过程,而失活则是单指数项过程,酶分子构象变化的快相速度常数与失活速度常数基本一致,因此我们认为二氢叶酸还原酶的盐酸胍变性过程中存在一个没有活力、但仍具备一定空间结构的变性中间体。

Regarding the speed along with the displacement even change's uniform non-uniform linear motion, its acceleration should define like this: At the unit displacement the speed change (the physics significance is: The speed along with the displacement change "the speed"), like this has:, the acceleration unit should be , Expression speed change which causes in proportion to as a result ,of the speed action of force.if the final velocity is 0, acts according to , namely .

对于速度随位移均匀变化的匀变速直-运动,它的加速度应该这样定义:在-位位移上速度的变化(物理意义是:-速度随位移变化的"快慢"),这样-有:,加速度的单位应该是,表示由于正比于速度的力作用而引起-速度的变化,其中。如果末速度为0,则根据,即。

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I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像,后来回台湾,甚至去年在北京,居然也四处看见她的海报,才惊讶她的魅力之大。

Ah may dee:You are chinese living in a democratic country.

你是居住在民主国家的中国人吧。