切下

"When that lawn mower cuts off your toes, don't come running to me."

"当割草机切下你的脚趾头的时候，你别跑来找我。"

Today it is the 11th anniversary for the loss of my little finger.

今天是手指被切下的11周年纪念日，是寻找之路的真正起步。

"Aye," said Guy,"proof enough. We will have Locksley back for the Abbey, and we will have, too, the hand of Master Robin chopped from him, or I think, with a word from Abbot Hugo, I may get leave to tear out his eyes. The Saxon hound has flouted us long enough, eh, Herbert?"

啊，」盖说：「有足够的证据我将使拉西里的土地归还寺院，而且我们将切下罗宾主人的手，或者，我想，依据雨果僧侣的话，我们将可好好地挖出他的双眼，这只撒克逊的狗愚弄我们已经够久了，是吧，赫伯特？

The Cre gene was cloned from lysogen BM25.8 by PCR. The Cre gene was then inserted in between the CaMV35S-35S double promtor and Nos terminator of PBI525 plasmid after sequencing. The expression box was cut down and inserted into plasmid pBINPLUS to obtain the final fertility-restoring expression vector pBINPLUSCre.

PCR方法从溶原菌BM25.8基因组DNA扩增出Cre基因，克隆测序验证无误后插入PBI525 CaMV35S-35S双启动子和Nos终止子之间，再将表达盒切下插入pBINPLUS质粒相应位点，得到恢复基因植物表达载体pBINPLUSCre。

After he had rested, he cut fillets from the dolphin and kept also the two flying fish he found in IT s maw.

休息一会之后，他切下几片海豚肉并且把在海豚胃中发现的两条飞鱼留了下来。

So he cut off the nose cone, hollowed it out, added in other parts to make it look like a jet and glued it onto his model.

然后，他就切下了那个鼻锥，把它挖空，然后和其它部件组合在一起，从而使它看上去像一个喷气引擎，然后再将这个喷气引擎模型粘到汽车模型上。

Facet joints oriented parallel to the sagittal plane provide substantial resistance to axial rotation but minimal resistance to shearing forces, whereas joints oriented more in a coronal plane tend to protect against flexion and shearing forces but provide minimal protection against rotation fig.

小关节朝向趋于矢状位可以限制脊柱的轴向旋转但是减少了对剪应力的限制。而趋于水平的小关节可以更好的保护屈曲和剪切下的应力，但对旋转的抵抗力降低（图3）。

The breed of gilt-edged wind pear basically is to use cuttage method; It has stronger cent to give birth to ability, after be being spent every year, chairman of phyllome radical department gives new shoot, in those days autumn can cut next enter growing in pearlite and vermiculite compound matrix, wait for rooted hind go up again basin conserve.

金边风梨的繁殖主要是采用扦插法；它有较强的分生能力，每年花后，叶丛基部会长出新芽，当年秋季就可切下栽入珍珠岩和蛭石混合的基质中，待生根后再上盆养护。

PAP-C23, amplified from mature pokeweed antiviral protein gene by PCR, was cloned into the expression vector PET101, and then the recombinant plasmid was transformed into Escherichia coli BL21(DE3). The recombinant mutant PAP was expressed high-levelly induced with IPTG.

用KCL溶液染SDS-PAGE胶，切下表达的目的蛋白，冰浴研磨后用磷酸缓冲液溶解，再加入等体积的福氏不完全佐剂，乳化完全后，免疫家兔，制备抗PAP蛋白的多克隆抗体，间接ELISA法测定所制备的抗血清的效价为1：1000。

Methods HIV-1 env gp41 gene of BH10 strain (nt6 977-7 497) was constructed into expressing vector pBV221 and expressed in E. Coli HB101. The expressed proteins were purified on 15% SDS-PAGE, the specific protein gel was cut down, transferred onto nitrocellulose membrane and stained with ponceau for 10 minutes.

表达产物通过15%SDS-聚丙烯酰胺凝胶电泳初步分离纯化，根据RF值，切下含特异蛋白的胶带，以Western blot法转移在硝酸纤维素膜上；免疫血清法检测合格者制备的抗原检测条带，经国家标准参比血清检测。

Do not gum up the work by telling mother what we are going to do .

别告诉母亲我们要干的事，那会把事情搞糟的。

In recent years, application of the partial prestressed concrete beam s is developed.

近年来，随着部分预应力砼梁应用的推广，发现我国规范所采用的名义拉应力法的计算结果不稳定。