查询词典 yeast-bitten

YAC is now prevailingly used as the first cloning vector for human genome analysis, but one problem that is not yet solved is the insert DNA fragment is not easily isolated from yeast chromosomes and vectors. So, we developed a new technique for the specific radioactive labelling of YAC insert by Alu-PCR technique.

因目前YAC中的插入片段还无法分离出来，因此我们对筛选到的这些YAC，用Alu-PCR技术将人基因组插入片段进行了特异扩增，并通过PCR条件的适当改变，获得较理想的标记效果。

Molecular mechanism for phase change of cell cycle has been studied in budding yeast, human cultured cells and procreant cells.

通过酵母、人培养细胞和生殖细胞三个系统研究了细胞周期时相驱动的分子机制。

In mice and tobacco, this was overcome by over-expression of prolyl hydroxylase, analogous to what has been done in yeast and insect cell culture.

在小鼠和烟草中，这是通过过渡表达聚羟化酶来实现的，类似于酵母和昆虫细胞培养。

A psychrophilic yeast (Y18) with the optimum and maximum growth temperatures of 10° C and 18° C ,respectively ,was isolated from Antarctica .

嗜冷酵母Y18分离自南极菲尔德斯半岛土壤样品。

Yeast two hybrid and TAP will be utilized to isolate the Pygopus binding proteins so that we elucidate the mechanisms of Pygopus in Wnt- dependent and independent pathways.

我们将利用酵母双杂交和TAP技术分离pygopus的结合蛋白，以彻底阐明其作用机理。

In this study we describe a novel strategy for assaying replicative lifespan in yeast, and apply it in a screening of mutants that are resistant to pro-oxidants.

在这项研究中，我们描述了一种新的战略寿命测定酵母中复制，并应用于筛选突变体的抗前氧化剂。

Objective To investigate the effects of yeast on reproductivity and growth of Drosophila melanogaster.

果蝇生殖周期短，实验操作简便，因而广泛用于老年学和遗传学的研究中。

To express chicken osteoprotegerin in Pichia pastoris and determine its inhibitive effects on mature osteoclastic bone resorptive function in vitro. The chOPG cDNA encoding for 382 amino acid without signal peptice was subcloned into Pichia pastoris expression vector pPICZα-A. The constructed plasmid was transformed into yeast X-33 by electroporation. The recombinant transformants were selected by Zeocin. Induced by the addition of methanol every 24 hours, the product analyzed by SDS-PAGE was sized about 43kDa and 53kDa at a yield of 200mg per litter of culture. The result of Western blotting indicated that the recombinant protein had specific antigenicity mainly owing to heterogeneous glycosylation.

为了在毕赤酵母中分泌表达鸡骨保护素（osteoprotegerin，OPG），采用DNA重组技术将chOPG成熟肽cDNA片段插入毕赤酵母表达载体pPICZα-A中，以电穿孔法转化酵母X-33，用Zeocin 平板筛选重组子，经甲醇诱导表达后， SDS-PAGE 和免疫印迹分析表达产物，由于糖基化不同，表达产物有两种，其相对分子量分别为43kDa和53kDa，表达量约为200mg/L，经Western印迹验证，有较好的抗原性。

We futher confirm their interaction in vivo through yeast retransformation assay, co-IP technique and colocalization method.

为了深入研究THAP11的作用抑制，我们利用酵母双杂交技术研究了THAP11的相互作用蛋白。

Maize syrup, a kind of brown sticky liquid is concentrated from the retted mixture. Its main contents are soluble protein, peptide and amino-acid etc., which is very nourishing. It can be used for producing antibiotic, gourment powder, yeast, inositol and etc.. So the maize syrup is a very important valuable by-product .

玉米浆是由浸渍水浓缩制得的，外观为棕褐色粘稠液体，主要成分大部分为溶解蛋白以及肽类、氨基酸等，有很高的营养价值，可用于生产抗生素、味精、酵母等产品的细菌营养料，同时还可制造菲汀、肌醇、植酸等，因此，玉米浆是重要的有价值副产品。

No, it's unnecessary. I don't think I have a fever.

不，没有必要，我想我没有发烧。

We live in a culture of instant gratification.

我们生活在即时满足的文化中。