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The greatest efficiency in Agrobacterium-mediated transformation of lettuce cotyledons(2～3 days-old) was obtained by 10-time dilution of Agrobacterium cells, 15-min inoculation time,1-day pre-culture,4-day co-culture and without AS using the MS(Murashige and Skoog\'s,1962) medium supplemented with 0.2 mg/L 6-benzylaminopurine,0.1 mg/Lα-naphthaleneacetic acid,100 mg/L Kan and 500 mg/L Carb,.

在农杆菌介导的皱叶生菜子叶(2～3 day苗龄)转化试验中，采用含BA 0.2mg/L，NAA 0.1 mg/L，Kan 100 mg/L，Carb 500 mg/L的MS培养基，最优的转化条件为农杆菌稀释倍数为10倍，预培养时间为1 day，共培养时间为4 day，浸染时间为15 min，不添加乙酰丁香酮。

Based on a computing model of floor heating residences, indoor thermal environment and heat distribution under the effect of heat charge/discharge, operation control and solar radiation has been concluded. Concept and formula of equivalent heat supply has been put forward which indicate that actual heat supply will be equal to the sum of design heat supply and equivalent heat supply. Recommend coefficient has been given taken Dalian as an example. Computed according to the new method, design heat supply can be reduced to 37% in cloudy day and 43.3% in sunny day, energy saving effect is very obvious. In Tromb wall solar house, effect of concrete wall on indoor temperature swing and attenuation of temperature wave is very obvious. While the outdoor air-temperature swing exceeds 10℃, indoor air-temperature swing belows 3℃. In the experiment, maximal difference in temperature between inner surface and outer surface of the 300mm wall exceeded 10℃. Heat storage and collector efficiency varies adversely according to solar radiation. Factors such as thickness, material, absorptance of the wall and permeation of the glass cover all have big influence on heat storage and collector efficiency. There exsisted an optimal thickness of the wall, which can make the best of heat storage and collector efficiency. Most of the heat stored in conventional Trombe wall during the daytime has been lost to the outside at night without heat preservation. Heat preservation on the outside surface of the wall has effectively improved heat release performance of the wall at night, heat supply to the room has increased too.

通过建立的地板采暖系统动态热性能分析模型，得出了在蓄放热特性、运行方式和太阳辐射等因素耦合作用下的室内热环境及热量分配比例，提出了等效供热量的概念及计算公式，指出实际所需供热量应等于设计供热量加上等效供热量，并以大连为例，给出了等效供热量的修正系数，以新方法计算，阴天可减少设计供热量37％，晴天最大可以减少设计供热量43.3％，节能效果显著；集热蓄热墙式太阳房中，混凝土蓄热墙的室温均一化效果和对温度波的削减作用非常明显，当室外温度波动最大幅度超过10℃时，室温波幅不超过3℃，实验中300mm厚墙体内外表面温差超过10℃；墙体蓄热效率与集热效率随太阳辐射照度的变化呈相反的变化趋势；墙体厚度、材料、表面吸收率以及盖板透过率等因素对集热和蓄热效率均有较大的影响，存在一个最佳的墙体厚度值，使得墙体集热和蓄热综合性能最优；传统的蓄热墙夜间没有外保温，辐射散热损失很大，研究表明墙体外保温方式明显改善了蓄热墙夜间的散热性能，增加了向室内的供热量。

Without method, oneself tried to change a map to make a tool, who knew to change had done not have weight of page of a day of content to fall completely, before snapshot of film of a lot of serialize turned N into the day, the map shows like that wait for calm, the content in the map is different nevertheless, the map that makes previously has time and address only, did not update frequency and first step, what use from the back is online generate a tool to have first step and newer frequency, because the map from the back has first step,knowing is inside the first grade with acquiescent page is reduced bring about fall authority or what are other reason, stationmaster is here people the place that ace gathers, experienced and knowledgeable, my map now is to show all the time treat calm condition, the station is Http://www.dy3166.cn, home page weight did not change, namely inside page weight was done not have, hope ace people help seek next accounts, declare, oneself are acclaimed by no means in this hair post, was to come up against this a Gordian knot really, hope ace people can help solve below, thanked.

没有办法，自己试着换了个地图生成工具，谁知道换了没过一天内容页权重全降了，好多连载影片快照都变成了N天前的，地图那一样显示待定，不过地图里的内容不一样，以前生成的地图只有时间和地址，没有更新频率和优先级，后面用的在线生成工具有优先级和更新频率，不知道是不是因为后面的地图有优先级把内页默认的优先级别降低导致的降权还是什么其他原因，这里是站长们高手云集的地方，见多识广，我的地图现在一直都是显示待定状态，站是http：//www.dy3166.cn，首页权重没有变，就是内页权重没有了，希望高手们帮忙找下原因，申明，本人在此发帖绝非炒作，是确确实实碰到了这个棘手的问题，希望高手们能够帮忙解答下，谢谢了。

Yesterday, the reporter contacted the netizen that realizes this desire, he appears the excitement of one face, he tells a reporter, all fools' day spending previously is in dull lie call the turn is spent, without new idea, spent all fools' day of one specific type however this year.

昨日，记者联系到了实现该愿望的网友，他显得一脸的兴奋，他告诉记者，以前过愚人节都是在无聊的谎言中度过，没有新意，今年却过了一个别样的愚人节。

Now I stand here,"airport without tears," my heart Whisper words "LOVE", in my heart "love and hope," co-exist, so I look forward to the day you can brave "expression of love", hope foolishly The sooner you can "find love", even if I ever left you, I will be "west" are waiting for you, hope you one day and eating "milk fried fritters"In the company of "freedom not changed" you go to the amusement.

现在，我站在这《不流泪的机场》，心里轻轻说着《L-O-V-E》，在我心中《爱与希望》并存，所以我期待着有一天能对你勇敢《表达爱》，希望傻傻的你能早点《发现爱》，即使我永远的离开了你，我也会在《西界》等着你，期望有一天能和你吃着《豆浆油条》，在陪着《自由不变》的你去游乐场。

This is real demonstration of love without biase mind or mind of the unknow because may watching the youtube video, you will find out that, everyone was so excited, jubilating, huging with free mind and i believe that day will be remarkable and unforgetable day more especially the woman that lose her daughter in a car accident.

这是真正的爱无演示Biase先生心中的想法或不明原因可能youtube观看录像、你会发觉，大家如此兴奋，jubilating，huging自由心相信那天会更加明显和难忘的一天，尤其是女子失去女儿车祸。

In that paragraph of day, he of the ground without use force sleeps to be not worn in the evening every day become aware, often one individual late hour loafs about in the ave lane of continous this world, those who feel unprecedented is spellbound with panic, feel oneself ideal is attacked so that smash by reality unexpectedly.

那段日子里，无用武之地的他天天晚上都睡不着觉，经常一个人半夜三更在绵阳的大街小巷游荡，感到前所未有的茫然和恐慌，觉得自己的理想竟被现实击得粉碎。

So she changed from thelarge house to the small one without any mark or difference;remained in her little room for the most part; pined silently;and died away day by day.

她从大房子搬到小房子，根本没有觉得有什么分别。大半的时间，她都闷在自己的小房间里默默地伤心，一天天地憔悴下去。

Somebody takes life for granted and muddles along day by day, without any sense of responsibility, they can never become successful merchants at all.

有的人对生活的解释就是混，这样的人，是不能做商人的。他没有责任感，他不懂得&负责&。

One day, when Sun Yang was passing a place, an old horse pulling acart loaded with salt suddenly neighed to him without stopping. He came near, and saw that it was a horse that really could cover a thousand Li a day, and that the only problem with it was that it was a little too old.

有一次，孙阳路过一个地方，忽见一匹拖着盐车的老马冲他叫个不停，走近一看，原来是匹千里马，只是年龄稍大了点。

Connectors are lines that can be anchored to particular places, called glue points , on the graphic object.

连接符，可固定在图形对象的特定的地方的线条。

Another way of putting it, too many agents have a say in each other's work, and bureaucratic rigor mortis sets in.

另一种表达是，太多的作用物在彼此的工作里都有话语权，官僚的僵尸开始抬头。