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Lastly, the support vector machine based on adjustive boundary is proposed, which is used to resolve the problem that the present support vector machines are sensitive to outliers too.

针对支持向量机对噪声数据过于敏感的问题，本论文提出了基于边界调节的支持向量机模型。

Neuroglobin, a recently identified monomer globin in nerve system, is highly affinitive with oxygen similar to myoglobin. After the coding region of rat NGB gene was sub cloned into the prokaryotic expression vector pGEX 4T 2, the recombinant vector was introduced into E. coli BL21 (DE3) cells for efficient expression.

氧是人类及许多生物赖以生存的重要条件，严重缺氧和低氧可危及人体生命。2 0 0 0年 9月，德国Burmester等人首次报道了人和小鼠脑内存在特异的功能上类似于肌红蛋白的携氧蛋白———脑红蛋白(neuroglobin ，NGB) [1 ] 。

Furthermore antisense plant expression vector of this gene was constructed using the pBI121 vector, laying foundation for studies on antisense regulation of ginseng β-amyrin synthase gene.

在此基础上，利用pBI121质粒载体，构建了人参β香树素合成酶基因的反义植物表达载体，为这一基因的反义调控研究打下基础。

ConclusionThe lentivirus RNAi vector of TREM-1 was constructed successfully. The lentivirus RNAi vector of TREM-1 can inhibit the expression of TREM-1 in the murine endotoxemia model caused by Bacteroides fragilis LPS.

成功构建了小鼠TREM-1基因RNAi慢病毒载体，所构建的慢病毒载体能够抑制脆弱类杆菌内毒素所诱导的TREM-1的表达。

An expression vector for one isoform of the mbC α1 subunit cDNA was constructed using the human cytomegalovirus promoter to direct expression and this expression vector was used in a novel transfection assay of primary cultures of bovine adrenal chromaffin cells.

为表达具有生物活性的钙离子通道，将mbC的一个异构体的蛋白编码区插入到由人的涎腺病毒启动子控制表达的质粒中。这个表达质粒

In order to simulate the transmissions of vector-borne diseases and discuss the related health policies effects on vector-borne diseases, we combine the multi-agent-based system, social network, mirror identity concept, and compartmental model to develop an epidemic simulation model.

此篇论文结合多代理人系统、人际接触社会网络、社会分身点概念、仓室模型等建模方法，发展一套适合用来模拟病媒性传染疾病的传播动态与探讨相关的公共卫生政策的成效的流行病电脑模拟模型。

By using the methods of real representation of complex matrices, companion vector and company vector, we study and solve the problems of Jordan canonical forms, triangular and generalized diagonalization for matrices under consimilarity.

我们通过复矩阵的实表示、友向量和伴向量方法，研究并解决了合相似意义下矩阵的若当标准形、合相似意义下矩阵的三角化和矩阵的广义对角化的问题。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

Methods The IL17 cDNA and Thy1.1 fulllength cDNA were subcloned into MIT(MSCVIRESThy1.1) retrovirus vector, and the siRNAIL17, U6 promoter and Thy1.1 fulllength cDNA were also inserted into retrovirus vector of pMNDBANSHEE.The recombined vectors were transfected 293 packaging cells by DNA calcium phosphate coprecipitation. Virus supernatant which infected preactivated spleen cells from NOD/BDC mice was collected. After incubation, the IL17 expression in diabetogenic T cells was detected.

利用基因工程和细胞克隆技术分别将IL17的cDNA插入MSCVIRESThy1.1逆转录病毒载体，将Thy1.1、U6增强子(U6 promoter)基因和IL17的siRNA cDNA插入逆转录病毒载体pMNDBANSHEE，采用磷酸钙沉淀法将重组载体转染293包装细胞；收集病毒上清，转染预先活化的NOD/BDC小鼠脾细胞，测定致糖尿病性T细胞的IL17和siRNAIL17的表达。

While in the transductive support vector machines all the test examples are treated equally, in the fuzzy transductive support vector machines, test examples are treated discriminatively according to their fuzzy labels. Thus, the adjustment of the decision hyperplane is refined, hence a more reliable decision function.

在直推支持向量机中，所有的测试样本被同等对待，而在模糊直推支持向量机中，根据其模糊类别标记的不同，测试样本得到区别对待，从而细化了分类决策面的调整过程，进而可以得到更为可靠的文本分类规则。

Chimborazo and Cotopaxi, took me by the hand.

越过琴博腊索山和科托帕克西山。

This car is in a good condition.

这辆车的状况很好。