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Further, with a semi-quantitative RT-PCR technique, the temporal expression of UuMAPKKK-like in U. unicinctus coelomic fluid cells was measured after stimulated by sulfide. The mRNA transcript of UuMAPKKK-like was low in the control and short time stressed (2 h, 6 h) groups, up-regulated gradually after 12h stimulation, and then reached its maximum level at 48h.

进一步采用RT-PCR技术对其在硫化物刺激前后的表达进行了检测，结果显示该基因在对照和应激后2h、6h的个体中表达较弱；刺激12h后表达量增高，并随应激时间增加，呈明显上调趋势。

Lower transcript of SSSⅡ-2 was constitutively expressed.

在5'端差异很大，可能由此赋予了不同的功能。

CML is cytogenetically marked by the philadelphia chromosome, which originates from a reciprocal translocation between chromosome 9 and 22 and is molecularly marked a chimeric bcr-abl gene, resulting from juxta-positive of the abl proto-oncogene on chromosome 9 with the bcr gene, which is normally located on chromosome 22. The chimeric bcr-abl gene expression an 8. 5kb hybrid mRNA transcript giving rise to a 210-KD fusion protein (P210〓) with increased tyrosine kinase activity. P210〓 plays a key role in the pathogenesis of CML. The continuous cell line K562 was established from the pleural effusion of a 53-year-old female with CML in terminal blast crisis, and was a human erythroleukemia line, contained Ph chromosome.

绝大多数慢粒患者白血病细胞中具有Ph染色体，是由9号染色体长臂3区4带和22号染色体长臂1区1带相互易位形成，即t（9；22），使位于9q〓的c-abl原癌基因在第二外显子的5'端断裂并易位到22 q〓的M-bcr基因第2或第3外显子的3'端，形成异常的bcr-abl嵌合基因，该基因转导出异常的mRNA，编码并翻译出P210蛋白，该蛋白具有很强的酪氨酸激酶活性，使粒细胞发生恶性增殖。K562细胞属于慢粒急变、红白血病细胞株，具有Ph染色体。

In most cases, these transcript pairs represent neighboring, divergently transcribed protein-coding genes that overlap for a region of their 3 ends.

在大多数情况下，这些谈话对周边代表、分歧转录蛋白质编码基因，它们的重叠区域为3结束。

But after 14 days of Cd exposure , the endogeic earthworm, Metaphire posthuma had lower transcript than two epigeic earthworms, Polypheretima elongata and Pontoscolex corethrurus.

镉暴露下14天后表层型蚯蚓Metaphire posthuma mt-2基因表现量低於两种深层型蚯蚓Polypheretima elongata与Pontoscolex corethrurus。

PGAD424 that has the activation domain of yeast'transcript activation factor GAL4 is a kind of shuttle vector that could replicate in both E. coli and yeast. One-sixth recombinant plasmids of this library could express fusion protein of GAL4-AD and proteins from Heliothis armigera.

通过在合成cDNA第一链时参入α〓PdCTP，显示出合成的cDNA为6.0Kb以下的连续区带，测得文库的滴度为1.5×10〓，可以代表亲本细胞的全部编码蛋白质的基因序列，即该文库具有代表性。

RT-PCR analysis showed that GhMADS1 gene expressed in petals, stamens, ovules and fibers, but not in roots, stems, leaves, bracts and sepals. The strongest expression of GhMADS1 gene was detected in petals. But in floral buds of a cotton homeotic mutant (CHV1), whose floral organs are all converted to bract leaf-like organs, the transcript of GhMADS1 gene was not detected.

RT-PCR分析显示，该基因在陆地棉的花瓣、雄蕊、胚珠和纤维中表达，特别是在花瓣中表达量最高，而在根、茎、叶等营养器官和棉花同源异型突变体CHV1（所有花器官均变为苞叶状叶性器官）的变异花蕾中不表达。

RT-PCR analysis showed the GhMASD1 gene expressed in petals, stamens, ovules and fibers, but not in roots,stems, leaves, bracts and sepals. The strongest expression of GhMADS1 gene was detected in petals. But in floral buds of a cotton homeotic mutant (CHV1), whose floral orgens are all converted to bract leaf-like organs, the transcript of GhMADS1 gene was not detected.

RT-PCR分析显示，该基因在陆地棉的花瓣、雄蕊、胚珠和纤维中表达，特别是在花瓣中表达量最高，而在根、茎、叶等营养器官和棉花同源异型突变体CHV1（所有花器官均变为苞叶状叶性器官）的变异花蕾中不表达。

While Washington marveled at the verbosity at the president's health-care summit Feb. 25, it was Mr. Knoller who put a number on it: at 60,000 words, the 155-page White House transcript of the summit was the longest ever recorded -- by Mr. Knoller, anyway. He says that as of Tuesday, Mr.

当华盛顿民众纷纷诧异于奥巴马于今年2月25日召集的医改峰会有多拖沓冗长时，克内勒及时地做出了统计：此次峰会六万字、155页的会议纪要是历次会议纪要中最长的一次──当然是在克内勒所统计的纪要范围之中。

Forensic text identification is a research that based on the analysis of suspicious text data which including suicide note, threatening letter, anonymous letter, the police transcript, suspicious e-mail, court record and the record that reproduced from phonetical material.

法律文本鉴别是一种基于对可疑文本语料，包括自杀遗书、恐吓信、匿名勒索信、警察笔录、可疑电子邮件，法庭审理记录及由语音材料转载的文本材料等语料的分析，寻找出文本作者，为法庭提供较为科学合理的研究报告的语言研究行为。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。